Primary structural analysis of the Tla region gene products, QA-2 and TL

Expression of hybrid class I genes of the major histocompatibility complex in mouse L cells.

“…The strategy for testing the expression capacity of the Qa genes is based on the pilot study of exon shuffling between the Q6 and H-2Ld genes (13). Although the intact Q6 gene is not expressed on the surface of mouse L cells after transfection, a hybrid gene in which the 5' portion of Q6 (exons [1][2][3] encoding the leader peptide and two external regions, a, and a2) was fused to the 3' portion (exons [4][5][6][7][8] expression potential of the QS, Q7, and Q8 genes, two sets of hybrid genes were constructed (Fig. 2).…”

“…The two types of class I proteins are structurally similar. Both have heavy chains of 38,000-45,000 daltons and are associated noncovalently with a 12,000-dalton light chain, (2-microglobulin (j32m), encoded outside the MHC (1,5,6). Inbred strains ofmice have distinct constellations of MHC alleles denoted by their H-2 haplotypes.…”

Effect of the major histocompatibility complex on early mouse development

“…Only one of the Qa-2 family has been biochemically characterized, Qa-2. Although Qa-2 is slightly smaller than the H-2K/D-L molecules, it is as similar to H-2K/D-L as K and D are to each other (Soloski et al, 1982). It is possible that all the Qa-2 family of antigens, with the exception of Qa-6, are antigenic specifici ties on one molecule.…”

“…^As compared to H-2K/D-L expression on lymphoid cells (Soloski et al, 1982(Soloski et al, ). et al, 1985.…”