Mouse strain and tissue distribution analyses indicate that the new antiserum A anti-A-Tlab recognizes the cell-surface product governed by the previously serologically undetectable Qa-1b allele. This cell-surface product has therefore been called Qa-1.2. Three levels of anti-Qa-1.2 cytotoxicity in the presence of complement have been observed: high, intermediate, and zero lysis. In general, high levels of lysis correlate with the presence of the Qa-1b allele, while zero levels of lysis correlate with the presence of the Qa-1a allele. The A.CA strain reacts with both anti-Qa-1.1 and anti-Qa-1.2 and may possess a third allele, Qa-Id. Several strains including B6-H-2k react in an intermediate fashion. Recombinant strain analyses indicate that this intermediate reaction may be due to modifying genes within the H-2D region.
We have used restriction enzyme digests, Southern blot analysis, and gene transfer experiments to identify a class I gene in the Q subregion of the murine major histocompatibility complex. By comparisons of class I genes from Q congeneic strains, five restriction fragment length polymorphisms were identified. Further studies of mutant (Qa-2-) and wild-type (Qa-
SUMMARY
The topographical relationship of Thy‐1, Ly‐1, Ly‐2, and T200 were examined on the murine thymocyte. The inhibition of the binding of radiolabelled monoclonal antibody after incubation with unlabelled heterologous antibody was used as a measure of the proximity of the target antigens on the cell surface. On unfixed cells, prior incubation with anti‐Thy‐1.2 impeded the attachment of labelled anti‐Ly‐1, anti‐Ly‐2, and anti‐T200. Anti‐T200, but not anti‐Ly‐1, or anti‐Ly‐2, was capable of impeding the attachment of labelled anti‐Thy‐1.2 on unfixed cells. In general, gentle fixation with paraformaldehyde did not alter these inter‐relationships. The possible functional contributions of such supramolecular relationships are discussed.
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