Primary, secondary and tertiary myotubes in developing skeletal muscle: A new approach to the analysis of human myogenesis

Myosin expression in semitendinosus muscle during fetal development of cattle: immunocytochemical and electrophoretic analyses

“…In all the buffers used in this procedure, bovine serum albumin was replaced by 3% (w/v) skimmed milk. (Draeger et al, 1987), in cats (Hoh et al, 1988), in rats (LaFramboise et al, 1991 in mice (Lyons et al, 1990) and in chickens (Stockdale and Miller, 1987). All the above studies confirmed that this expression is not necessarily dependent on innervation, which is not fully developed at this stage of growth.…”

“…The most detailed results have been obtained in birds (Bandman et al, 1982;Crow and Stockdale, 1986;Miller and Stockdale, 1986; for reviews, see Stockdale and Miller, 1987, and Stockdale, 1990), in small mammals, such as mice (Rubinstein and Kelly, 1981;Whalen et al, 1981;Lyons et al 1983;Vivarelli et al, 1988) and cats (Hoh and Hughes, 1989). Studies have also been made on the human fetus (Pons et al, 1986;Draeger et al, 1987 39,53,64,84,98,122,143,180,224 Pons et al (1986). We used 4 monoclonal antibodies (MAb) raised against different types of human myosin heavy chains: a p type (slow) myosin from the heart ventricle of an adult (antibody A), a fast myosin immunopurified from the quadriceps muscle of an adult (antibody B), a myosin from a 22-wk-old fetus (antibody C) and a myosin from adult atrium (antibody D).…”

“…However, it is now widely recognized that the last stage of myogenesis is dependent on environmental influence (see reviews of Stockdale, 1990;Hughes and Blau, 1992 . In a previous study on cattle (Robelin et (Draeger et al, 1987) (Pons et al, 1986;Draeger et al, 1987) (Pons et al, 1986;Draeger et al, 1987 (Holmes and Ashmore, 1972;Johnston et al, 1975;Robelin, unpublished data …”

Muscle fibre ontogenesis in farm animal species

“…The existence of a third generation of fibres has been described in the sheep [100], pig [46,53], human [21] and bovine [23]. These cells exhibit a small diameter [26,52], and usually express developmental MHC isoforms [46].…”

“…These cells exhibit a small diameter [26,52], and usually express developmental MHC isoforms [46]. When first formed, they are closely associated with secondary myotubes like newly formed secondary myotubes with primary myotubes [21]. They are observed at about 40% of the gestation period in bovines, sheep and humans [21,23,100] (Tab.…”