Primary fibroblast cultures and karyotype analysis for the olive ridley sea turtle (Lepidochelys olivacea)

The current state and future directions of marine turtle toxicology research

“…Skin fibroblast cell lines are the most common, and have been established for four different species -C. mydas (one individual, Lu et al, 1999), E. imbricata (multiple individuals, Fukuda et al, 2012), C. caretta (multiple individuals, Webb et al, 2014) and L. olivacea (two individuals, Fukuda et al, 2014). Skin epithelioid cells have also been established for C. mydas (one individual, Koment and Haines, 1982).…”

“…Testis by ; brain, periorbital tissue, heart, fibropapilloma, lung, liver, spleen, testis, urinary bladder by Tan et al (2010); liver, periorbital tissue, lung, kidney, heart, brain, testis by Wang et al (2013a) Moore et al (1997) C. mydas Embryo fibroblast, heart, liver, kidney, brain, eye, lung Embryo fibroblast by Tan C. caretta Skin fibroblast Used in same study Koment and Haines (1982) C. mydas Skin epithelioid Mansell et al (1989) C. mydas Fibropapilloma fibroblast Fukuda et al (2014) L. olivacea Skin fibroblast Table 6 Breakdown of the toxicity studies investigating biomarkers of exposure in marine turtles. review, it was evident that marine turtle research is heavily focused towards two species: C. mydas and C. caretta.…”

Expression of human mutant cyclin dependent kinase 4, Cyclin D and telomerase extends the life span but does not immortalize fibroblasts derived from loggerhead sea turtle (Caretta caretta)

“…Nakamura reported that chromosome number of loggerhead was 2 n = 58 17 . On the other hand, the chromosome number of the green sea turtle ( Chelonia mydas ) 23 , olive ridley sea turtle ( Lepidochelys olivacea ) 24 , 25 and the hawksbill sea turtle ( Eretmochelys imbricata ) 16 were consistently reported to be 2 n = 56. Hybridisation between multiple strains of sea turtle, including the loggerhead, has previously been demonstrated 4 , 26 – 29 .…”

“…The karyotype analysis of established primary and recombinant cell lines was conducted following a previously described method 25 , 39 . For the determination of chromosome number and banding pattern, we used two primary cell lines: A634 and A640.…”

Establishment of primary cell lines from tissues of the red-eared slider

“…For reptilia, сommercial cell lines are in general limited to one chelonian cell line TH-1 from the terrestrial turtle Terrapene carolina (https://www.lgcstandardsatcc.org/products/all/CCL-50.aspx). However, the literature indicates that primary somatic cell lines have been established and reported for several Testudines species, such as the Galapagos giant tortoise Geochelone nigra (Goldstein, 1974), green turtle Chelonia mydas (Mansell, Jacobson, and Gaskin, 1989;Lu et al, 1999), snapping turtle Chelydra serpentina, painted turtle Chrysemys picta, mud turtle Kinosternon flavescens (Christiansen et al, 2001), hawksbill sea turtle Eretmochelys imbricata (Fukuda et al, 2012), olive ridley sea turtle Lepidochelys olivacea (Fukuda et al 2014), loggerhead sea turtle Caretta caretta (Webb et al, 2014;Fukuda et al, 2018), and Chinese soft-shelled turtle Pelodiscus sinensis (Guo et al, 2016). Establishment of an ovarian stem-like cell line from the P. sinensis was recently described (Xu et al, 2018).…”