Primary cultures of embryonic cells from the snail Biomphalaria glabrata *

Basch, Paul F.; DiConza, Joseph J.

doi:10.4269/ajtmh.1973.22.805

Cited by 14 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Unfortunately these efforts yielded no cell lines. During this time, Eder L. Hansen (Hansen 1976) also used embryos to initiate cell cultures that exhibited characteristics similar to those described by Basch and Diconza (1973), but also noted new colonies of adherent, fibroblast-like cells that continued to proliferate upon subculture and multiple passages. These continuously propagating cells became known as the Bge cell line.…”

Section: Molluscan Cell Linesmentioning

confidence: 89%

“…Because of its medical importance, the freshwater snail Biomphalaria glabrata Say, 1818 has been intensely studied. Basch and DiConza (1973) used chemical dissociation of embryo fragments to create primary cell cultures using various complex media. Several cell types were observed including fibroblast-like and epithelioid-type cells early (3–7 days) in culture, later giving way to “polygonal cells” at 2–3 weeks, and finally “muscle” cells dominating cultures at 4–5 weeks in culture.…”

Section: Molluscan Primary Cell Culturesmentioning

confidence: 99%

“…Over a 3-year contract period, successful primary cultures were established from larval stages (whole egg masses or separated capsules containing trochophore-stage embryos) of B. glabrata. Cells that persisted in vitro for six weeks included fibroblast-like cells, contractile muscle cells, and epithelial-like cells (Basch and DiConza 1973). Primary cell cultures of explanted heart and gonad tissues from adult snails also were established, some lasting for >1 year (Bayne et al 1975).…”

Section: Molluscan Cell Linesmentioning

confidence: 99%

See 2 more Smart Citations

Molluscan cells in culture: primary cell cultures and cell lines

2013

View full text Add to dashboard Cite

In vitro cell culture systems from molluscs have significantly contributed to our basic understanding of complex physiological processes occurring within or between tissue-specific cells, yielding information unattainable using intact animal models. In vitro cultures of neuronal cells from gastropods show how simplified cell models can inform our understanding of complex networks in intact organisms. Primary cell cultures from marine and freshwater bivalve and gastropod species are used as biomonitors for environmental contaminants, as models for gene transfer technologies, and for studies of innate immunity and neoplastic disease. Despite efforts to isolate proliferative cell lines from molluscs, the snail Biomphalaria glabrata Say, 1818 embryonic (Bge) cell line is the only existing cell line originating from any molluscan species. Taking an organ systems approach, this review summarizes efforts to establish molluscan cell cultures and describes the varied applications of primary cell cultures in research. Because of the unique status of the Bge cell line, an account is presented of the establishment of this cell line, and of how these cells have contributed to our understanding of snail host-parasite interactions. Finally, we detail the difficulties commonly encountered in efforts to establish cell lines from molluscs and discuss how these difficulties might be overcome.

show abstract

Section: Molluscan Cell Linesmentioning

confidence: 89%

Section: Molluscan Primary Cell Culturesmentioning

confidence: 99%

Section: Molluscan Cell Linesmentioning

confidence: 99%

See 1 more Smart Citation

Molluscan cells in culture: primary cell cultures and cell lines

2013

View full text Add to dashboard Cite

show abstract

“…It seem that inoculation of cells number in primary culture is a debatable point. In cultivation of embryonic cells from B. glabrata reported by Basch and Diconza (1973) and , they observed at least three types cells, that is, amebocytic, epithelial like and fibroblastic like cells, and a cell line designated Bge ( Biomphalaria embryo) that has been maintained through many subcultures of colonies of epithelial and/or fibroblastic like cells. In our observations of the tissues culture in the present study, three types of cells, i.e., amebocytic, epithelial like and fibroblastic like cells were observed near margins of explanted tissue.…”

Section: Resultsmentioning

confidence: 99%

“…It seem that amount of amino acids components in medium is the important factor for long-term culture. Basch and Diconza (1973) maintained embryonic cells from B. glabrata with medium 199 and components from analyses of hemolymph from adult B. glabrata , but the cell could not maintained long-term. It was considerable that amount of total amino acid components of the hemolymph in medium was not enough during the culture period.…”

Section: Diluted (X2) Git Medium---------------------200ml 2 Aminomentioning

confidence: 99%

In vitroCultivation of Cells from Ovotestis Tissue of Pigmented Biomphalaria glabrata

Iwanaga¹

2002

Zoological Science

View full text Add to dashboard Cite

Biomphalaria Snails and Larval Trematodes

Toledo

Fried

2011

View full text Add to dashboard Cite

), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights.Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com) v The history of research on the relationships between Biomphalaria spp. and larval trematodes, particularly Schistosoma mansoni, is long, active, and ongoing today. Snails of the genus Biomphalaria are of significant medical importance with many species living in freshwater habitats associated with human settlements; many of these snail species are obligate intermediate hosts of the human blood fluke S. mansoni, the causative agent of hepatosplenic schistosomiasis. This parasitic disease continues to disrupt the lives of about 200 million people in over 70 countries and prevents these individuals from otherwise reasonable expectations of healthy and productive lives. Furthermore, it is estimated that within the developing world, especially in sub-Saharan Africa, more than 700 million people are at risk of becoming infected, despite the efforts to control transmission in human and snail populations by mass chemotherapy and the use of molluscicides. Several factors, such as the absence of a schistosome vaccine, the recent appearance of resistance to antischistosome drugs, and human activities that expand snail habitats, have increased the need for a better understanding of schistosome-snail interactions. In recent years, the application of new technologies has contributed to the accumulation of considerable new information on this topic that may be of great use to biomedical scientists.In addition to the impact of Biomphalaria spp. on public health, these snails are also interesting models for the study of other topics such as population biology, including genetics and demography, proteomics, invertebrate immunobiology, mating systems, and biogeography, among others. Biomphalaria spp. snails have been extensively used as experimental biological models contributing significantly to new developments in many areas studied by biomedical scientists. Extensive coverage of these topics is included in this book, also considering trematode species other than schistosomes.The aim of the present book is to provide an overview of the recent advances in the Biomphalaria spp.-larval trematode interactions, especially in Biomphalariaschistosome systems. Emphasis is placed on gaps in our knowledge that must be filled to gain a better understanding of the relationships in these host-parasite systems. This may be critical for a deeper knowledge of the transmission of schistosomiasis and other snail-borne parasitic diseases. Pref...

show abstract

Primary cultures of embryonic cells from the snail Biomphalaria glabrata *

Cited by 14 publications

References 0 publications

Molluscan cells in culture: primary cell cultures and cell lines

Molluscan cells in culture: primary cell cultures and cell lines

In vitroCultivation of Cells from Ovotestis Tissue of Pigmented Biomphalaria glabrata

Biomphalaria Snails and Larval Trematodes

Contact Info

Product

Resources

About