<i>In vitro</i>Cultivation of Cells from Ovotestis Tissue of Pigmented Biomphalaria glabrata

Iwanaga, Yuzuru

doi:10.2108/zsj.19.271

Cited by 4 publications

(5 citation statements)

References 10 publications

(9 reference statements)

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“…Snail cells have been cultured in different media and have been shown to be tolerant to wide ranges of osmolality [Bayne et al, 1975;Benex and Gantier, 1975;Benex and Jacobelli, 1980;Hansen, 1976;Iwanaga, 2002;Barbosa et al, 2006b]. Nevertheless, the Bge cell line was established under low osmolality conditions [Hansen, 1976].…”

Section: Viability Of Cells In Culturementioning

confidence: 99%

“…Cells resembling hemocytes were previously described in cultures from heart, saccular kidney and APO of B. glabrata, mainly based on the amorphous shape of those cells under optical microscopy [Bayne et al, 1975;Benex and Gantier, 1975;Benex and Jacobelli, 1980;Hansen, 1976;Iwanaga, 2002;Barbosa et al, 2006b]. However, we preferred not to classify the cells in culture according to any shape-based criteria.…”

Section: Cell Interaction With the Parasitementioning

confidence: 99%

“…Cells from primary APO-derived cultures from the Taim strain of B. tenagophila were able to eliminate S. mansoni sporocysts in vitro, whereas those from a susceptible strain reacted weakly against S. mansoni sporocysts and failed to recognize the parasite as non-self [Nacif-Pimenta et al, 2012]. Primary cell cultures from other Biomphalaria organs-such as kidney, heart, digestive gland and ovotestis-were previously obtained, but were examined by optical microscopy only, and have thus not been fully characterized, except for cell shape and substrate adherence [Bayne et al, 1975;Benex and Gantier, 1975;Benex and Jacobelli, 1980;Hansen, 1976;Iwanaga, 2002;Barbosa et al, 2006b]. A more comprehensive characterization of Biomphalaria sp.…”

Section: Introductionmentioning

confidence: 99%

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Ultrastructural characterization of cells in primary cultures from different adult tissues of Biomphalaria tenagophila TAIM, a strain that is absolutely resistant to Schistosoma mansoni infection

Silva-Neto

Silva

Alves

et al. 2014

Micron

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Section: Viability Of Cells In Culturementioning

confidence: 99%

Section: Cell Interaction With the Parasitementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ultrastructural characterization of cells in primary cultures from different adult tissues of Biomphalaria tenagophila TAIM, a strain that is absolutely resistant to Schistosoma mansoni infection

Silva-Neto

Silva

Alves

et al. 2014

Micron

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“…In addition, primary cultures of B. glabrata ovotestis (Iwanaga 2002;Barbosa et al 2006) and amebocyte-producing organ (APO) (Barbosa et al 2006) have been carried out. The ovotestis cultures grew two cell types (Iwanaga 2002) and those of the APO grew three cell types (Barbosa et al 2006).…”

Section: In Vitro Culture Of Snail Tissues and Cellsmentioning

confidence: 99%

“…The ovotestis cultures grew two cell types (Iwanaga 2002) and those of the APO grew three cell types (Barbosa et al 2006). …”

Section: In Vitro Culture Of Snail Tissues and Cellsmentioning

confidence: 99%

Laboratory Rearing of Biomphalaria glabrata Snails and Maintenance of Larval Schistosomes In Vivo and In Vitro

Eveland

Haseeb²

2010

Biomphalaria Snails and Larval Trematodes

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This chapter describes the methods used by the current authors and numerous other investigators to optimize conditions for successfully growing and maintaining both uninfected and infected Biomphalaria glabrata in the laboratory, with particular emphasis on the optimal physical, chemical, and biological factors that affect successful snail rearing and/or breeding, and the ways that they can be measured. Consideration is also given to incidental organisms that can alter aquarium ecology and affect both the snails and their intended trematode parasites. Various methods of infecting snails and maintaining snail tissues and cells in vitro are also described. The goal of this chapter is to aid researchers who have the need or desire to maintain the life cycle of this pulmonate and its trematode parasites in the laboratory.

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Biomphalaria Snails and Larval Trematodes

Toledo

Fried

2011

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), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights.Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com) v The history of research on the relationships between Biomphalaria spp. and larval trematodes, particularly Schistosoma mansoni, is long, active, and ongoing today. Snails of the genus Biomphalaria are of significant medical importance with many species living in freshwater habitats associated with human settlements; many of these snail species are obligate intermediate hosts of the human blood fluke S. mansoni, the causative agent of hepatosplenic schistosomiasis. This parasitic disease continues to disrupt the lives of about 200 million people in over 70 countries and prevents these individuals from otherwise reasonable expectations of healthy and productive lives. Furthermore, it is estimated that within the developing world, especially in sub-Saharan Africa, more than 700 million people are at risk of becoming infected, despite the efforts to control transmission in human and snail populations by mass chemotherapy and the use of molluscicides. Several factors, such as the absence of a schistosome vaccine, the recent appearance of resistance to antischistosome drugs, and human activities that expand snail habitats, have increased the need for a better understanding of schistosome-snail interactions. In recent years, the application of new technologies has contributed to the accumulation of considerable new information on this topic that may be of great use to biomedical scientists.In addition to the impact of Biomphalaria spp. on public health, these snails are also interesting models for the study of other topics such as population biology, including genetics and demography, proteomics, invertebrate immunobiology, mating systems, and biogeography, among others. Biomphalaria spp. snails have been extensively used as experimental biological models contributing significantly to new developments in many areas studied by biomedical scientists. Extensive coverage of these topics is included in this book, also considering trematode species other than schistosomes.The aim of the present book is to provide an overview of the recent advances in the Biomphalaria spp.-larval trematode interactions, especially in Biomphalariaschistosome systems. Emphasis is placed on gaps in our knowledge that must be filled to gain a better understanding of the relationships in these host-parasite systems. This may be critical for a deeper knowledge of the transmission of schistosomiasis and other snail-borne parasitic diseases. Pref...

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In vitroCultivation of Cells from Ovotestis Tissue of Pigmented Biomphalaria glabrata

Cited by 4 publications

References 10 publications

Ultrastructural characterization of cells in primary cultures from different adult tissues of Biomphalaria tenagophila TAIM, a strain that is absolutely resistant to Schistosoma mansoni infection

Ultrastructural characterization of cells in primary cultures from different adult tissues of Biomphalaria tenagophila TAIM, a strain that is absolutely resistant to Schistosoma mansoni infection

Laboratory Rearing of Biomphalaria glabrata Snails and Maintenance of Larval Schistosomes In Vivo and In Vitro

Biomphalaria Snails and Larval Trematodes

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