Prickly palm cactus husk as a raw material for production of ligninolytic enzymes by Aspergillus niger

Abstract

Prickly palm cactus husk was used as a solid-state fermentation support-substrate for production of the ligninolytic enzymes laccase, peroxide manganese, and lignin peroxidase by . Effects of water activity, temperature, and fermentation time on enzymatic production were evaluated using a central composite rotatable design. Response surface methodology revealed that maximum enzyme production was achieved at 73.38 h of fermentation, a water activity of 0.87 A, at 28.74°C for laccase, at 65.33 h, 0.89 A, and 28.96°C for lignin peroxidase, and at 70.44 h, 0.91 A, and 28.84°C for manganese peroxidase. Optimized enzyme production was 9,023.67 UI/L for laccase, 2,234.75 UI/L for lignin peroxidase, and 8,534.81 UI/L for manganese peroxidase. Thermostability and pH stability were observed for all enzymes. Enzymatic deactivation kinetic experiments indicated that enzymes remained active after freezing of crude extracts.

Effect of the solid state fermentation of cocoa shell on the secondary metabolites, antioxidant activity, and fatty acids

“…In our research group, several bioactive compounds have been identified in the cocoa shell (data not presented). In addition, the cultivation of fungi in waste food has been used of production of microbial enzymes [12,15,16]. Here we aimed to investigate the effect of solid state fermentation by P. roqueforti on the secondary metabolites, antioxidant activity, and fatty acids present in the cocoa meal.…”

Lignocellulolytic Potential of the Recently Described Species Aspergillus olivimuriae on Different Solid Wastes

“…Among the non-amended cultures, the best laccase and peroxidase production levels were found in RTB and SCG; in these growth substrates, the presence of WB led to significantly increased laccase and peroxidase activities. However, on the tested substrates, the production levels of these enzymes were markedly lower than those observed in solid-state cultures of other Aspergillus species, such as A. fumigatus SCBM6 and A 1004, grown on palm cactus husk, yielded laccase activity, which was around 173-fold higher than the best production achieved with A. olivimuriae [66].…”

Overview of Mycelial Fungi - Lignin Destructors

“…Aspergillus niger is also referred to as a producer of laccase, peroxide manganese and lignin peroxidase. In the study [17], the production of enzymes was optimized, the activity of which was 9023,67 UI/L for laccase, 2234,75 UI/L for lignin peroxidase, and 8534,81 UI/L for manganese peroxidase.…”

“…In addition to agricultural waste substrates containing lignin, which include, for example, wheat straw, corncobs [12], palm cactus husk [17], artificially synthesized "model" compounds that resemble parts of the proposed formula of native lignin can also be used for research.Thus, in the article [2], the biodegradation of the azo dye malachite green (MG) using the culture of the fungus Aspergillus flavus (F10), which secretes laccase and manganese peroxidases, was considered, as a result of which discoloration of the substrate is observed.It is worth noting the difference in temperature and pH optima for the accumulation of a specific enzyme from this type offungy: for laccase(Lac)it is 7,5 pH and 15 ∘ C. For LiP and MnP, the pH is 7,5-9,5 and 5,5-9,5 and 25 and 35 ∘ C, respectively [4]. The introduction of sodium nitrate into the medium can induce their synthesis [1,2].…”