Preventive role of genistein in an experimental non‐alcoholic steatohepatitis model

Yalnız, Mehmet; Bahçecıoğlu, İbrahim Halil; Kuzu, Nalan; Poyrazoğlu, Orhan Kürşat; Bulmuş, Özgür; Çelebi, Selman; Üstündağ, Bilal; Özercan, İbrahim; Şahin, Kazım

doi:10.1111/j.1440-1746.2006.04681.x

Cited by 59 publications

(35 citation statements)

References 36 publications

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“…They also suggested that isoflavone supplementation in broilers may be needed to protect tissues against attack by lipid oxidation products. In addition, in response to genistein supplementation, liver and serum MDA levels decreased in quails (Onderci et al, 2004) and in rats (Yalniz et al, 2007).…”

Section: Discussionmentioning

confidence: 97%

Genistein supplementation to the quail: Effects on egg production and egg yolk genistein, daidzein, and lipid peroxidation levels

Akdemir

Şahin

2009

Poultry Science

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Genistein, a soy phytoestrogen, is a powerful antioxidant. In the present study, we investigated the effects of dietary genistein supplementation on Japanese quail (Coturnix coturnix japonica) laying performance and egg yolk contents of malondialdehyde (MDA), vitamin A, and vitamin E. Malondialdehyde is an indicator of lipid peroxidation, whereas vitamins A and E have antioxidant properties. Birds (n=150; 5 wk old) were randomly assigned to 1 of 3 groups consisting of 50 birds (5 replicates of 10) and were fed a basal diet or the basal diet supplemented with either 400 or 800 mg of genistein/kg of diet. The experimental period lasted 90 d with a 17L:7D photo schedule. As antioxidant indices, yolk MDA and vitamin (A and E) concentrations were measured by HPLC. Dietary genistein supplementation (800 mg/kg) increased feed intake, egg production, egg weight, Haugh unit, shell thickness, and shell weight and improved feed efficiency at a greater extent than the other levels (0 and 400 mg/kg). Egg yolk genistein concentration was increased (P<0.0001), whereas egg yolk MDA concentration was decreased (P<0.0001) at the highest level of genistein supplementation. However, genistein supplementation did not affect egg yolk daidzein, vitamin A, and vitamin E levels. There was an inverse relationship between egg yolk genistein and MDA concentration (y=0.02xe(genistein), R2=0.74, P<0.0001). Results of the present study indicate that supplementing with dietary genistein (800 mg/kg) improved performance, egg quality, and egg yolk genistein content and decreased egg yolk MDA concentration in quail.

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Section: Discussionmentioning

confidence: 97%

Genistein supplementation to the quail: Effects on egg production and egg yolk genistein, daidzein, and lipid peroxidation levels

Akdemir

Şahin

2009

Poultry Science

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“…Wei et al (50) reported that genistein strongly inhibited formation of reactive oxygen species and thus cell-derived hydrogen peroxide formation in vivo. Yalniz et al (51) reported that dietary genistein supplementation decreased plasma and liver MDA concentrations in rats. Similar to our results, about 20% decrease in plasma 8-isoprostane levels was detected when textured soy protein containing 56 mg soy isoflavones was consumed daily for 2 wk by healthy volunteers (52).…”

Section: Discussionmentioning

confidence: 98%

Inhibitory Effects of Combination of Lycopene and Genistein on 7,12- Dimethyl Benz(a)anthracene-Induced Breast Cancer in Rats

Şahin¹,

Tuzcu²,

Şahin³

et al. 2011

Nutrition and Cancer

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Breast cancer is one of the most common cancers in women. Carotenoids and soy isoflavones have been postulated to have breast cancer preventive effects. We investigated the potential preventive effects of lycopene and genistein, alone and in combination, on breast cancer development in female Wistar rats treated with 7,12-dimethylbenz[a]anthracene (DMBA), a carcinogen known to induce breast tumors. Mammary carcinogenesis was initiated by a single, oral gavage of DMBA (80 mg/kg body weight) at 55 days of animal age. Fifty female Wistar rats were divided into 5 experimental groups having 10 animals per group: Group 1 (normal control), Group 2 (DMBA control), Group 3 (DMBA + lycopene), Group 4 (DMBA + genistein), and Group 5 (DMBA + lycopene and genistein). Rats were fed either lycopene (20 mg /kg bw) or genistein (2 mg /kg bw) by oral gavage (3 times per week) starting 2 wk prior to DMBA injection. Treatment was continued for 20 wk. Rats treated with DMBA developed mammary tumors with 100% tumor incidence during the 20-wk study. Inhibition of mammary cancer incidence by lycopene (70%), genistein (60%) and their combination (40%) was observed. Tumor weight decreased by 48%, 61%, and 67%, and mean tumor volume decreased by 18%, 35%, and 65% with lycopene, genistein, and lycopene + genistein, respectively (P < 0.01 for the combination). The proportions of adenocarcinoma masses decreased with lycopene and genistein combination (P < 0.05). Administration of lycopene and genistein combination suppressed breast cancer development and was associated with a decrease in MDA, 8-isoprostane, and 8-OhdG levels and with an increase in serum lycopene and genistein levels. Animals administered DMBA developed breast cancer, which was associated with increased expression of Bcl-2 and decreased expression of Bax, caspase 3, and caspase 9 in mammary tissues. Administration of genistein and lycopene in combination was more effective in inhibiting DMBA-induced breast tumors and modulating the expression of apoptosis associated proteins than the administration of each agent alone. Our results suggest that lycopene and genistein are potent antioxidants and, when given in combination, offer maximum protection against DMBA-induced mammary carcinogenesis.

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“…Small, mostly animal, studies suggest it has antioxidant properties and can improve insulin sensitivity and reduce hepatic fat content (101). Genistein, a soy isoflavone, has been shown to improve insulin sensitivity in insulin resistant animal models and improved oxidative stress in animals with NASH (102)(103)(104). Studies in human beings have not been published and, thus, nutritional supplementation with genistein cannot be recommended.…”

Section: Other Nutrients That May Have Hepatoprotective Effectsmentioning

confidence: 98%

The Role of Diet and Nutrient Composition in Nonalcoholic Fatty Liver Disease

McCarthy

Rinella²

2012

Journal of the Academy of Nutrition and Dietetics

140

123

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Preventive role of genistein in an experimental non‐alcoholic steatohepatitis model

Abstract: Genistein, a strong antioxidant agent, significantly decreased the plasma TNF-alpha level and remarkably prevented the emergence of NASH by improving the biochemical and histopathological abnormalities via attenuating oxidative stress.

Cited by 59 publications

References 36 publications

Genistein supplementation to the quail: Effects on egg production and egg yolk genistein, daidzein, and lipid peroxidation levels

Genistein supplementation to the quail: Effects on egg production and egg yolk genistein, daidzein, and lipid peroxidation levels

Inhibitory Effects of Combination of Lycopene and Genistein on 7,12- Dimethyl Benz(a)anthracene-Induced Breast Cancer in Rats

The Role of Diet and Nutrient Composition in Nonalcoholic Fatty Liver Disease

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