Preventive role of estradiol on kidney injury induced by renal Ischemia-Reperfusion in male and female rats

Irannejad, Akram; Nematbakhsh, Mehdi; Eshraghi-Jazi, Fatemeh; Talebi, Ardeshir

doi:10.4103/2008-7802.153537

Cited by 26 publications

(19 citation statements)

References 39 publications

(45 reference statements)

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“…In addition, protective effects of testosterone were approved in renal ischemia-reperfusion model (47). Similarly, a recent study detected that estrogen 500 mg/kg decreased renal damage in ischemia-reperfusion model and its administration improved proteinuria and renal damage (48). A robust finding of this study was the increase of renal SOD activity in female rats after the administration of the vitamins, while this effect was not observed in male rats.…”

Section: The Effect Of Gentamicin On Kidney Mda Level and Kidney Sod supporting

confidence: 55%

Gender differences in response to vitamin E and C in gentamicin induced nephrotoxicity in Wistar rats

Safari¹,

Miri²,

Ghofran³

et al. 2017

J Nephropathol

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Background: Nephrotoxicity is the most recognized side effect of gentamicin. Vitamin E and vitamin C demonstrate their effective role in the prevention of nephrotoxicity. Likewise, previous studies have suggested that women have low risk of end-stage renal disease at premenopausal period. Objectives: This study aims to investigate the possibility of any gender difference in response to antioxidant effects vitamins E and C in gentamicin-induced nephrotoxicity. Materials and Methods: Wistar rats were randomly assigned to 6 groups each including both male and female rats. The first and second groups received saline (control group) and almond oil, the third group received gentamicin. The fourth group received a regular dose of gentamicin + vitamin E. Similarly, the fifth group received a regular dose of gentamicin + vitamin C. The sixth group received a dose of gentamicin + vitamin C and E simultaneously constantly. This protocol continued for 9 days. Results: Gentamicin increased significantly urea, creatinine (Cr) and malondialdehyde (MDA), but it decreased superoxidase dismutase (SOD) level (P < 0.05). Treatment with antioxidant vitamins improved urea, creatinine, MDA, and SOD serum level significantly in both genders (P <0.05). Likewise, kidney MDA level enhanced significantly (P <0.05) and treatment with antioxidant vitamins reduced MDA level too (P <0.05). Gentamicin decreased kidney SOD activity in male and female rats (P <0.05). However, treatment with antioxidant vitamins did not improve its level in male rats, while in female rats, vitamins E and C compensated for kidney SOD activity. Conclusions: Antioxidant vitamins modified gentamicin-induced nephrotoxicity in both genders, with some difference in response to vitamins E and C between the genders, that was higher in female rats.

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Section: The Effect Of Gentamicin On Kidney Mda Level and Kidney Sod supporting

confidence: 55%

Gender differences in response to vitamin E and C in gentamicin induced nephrotoxicity in Wistar rats

Safari¹,

Miri²,

Ghofran³

et al. 2017

J Nephropathol

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“…Takaoka et al [ 34 ] and Tanaka et al [ 35 ] demonstrated with the renal pedicle occlusion model that male rats injected with estrogen 15 min prior to reperfusion demonstrated improved post-ischemic renal outcomes. On the other hand, a recent study which employed intramuscular injection of estrogen 3 days prior to renal ischemia-reperfusion surgery found that estrogen’s protective effect was limited to ovariectomized female rats and did not protect male rats [ 36 ]. The discrepancy of these findings is probably due to the heterogeneity of the experimental model and the length of time of administration.…”

Section: Discussionmentioning

confidence: 99%

“…The discrepancy of these findings is probably due to the heterogeneity of the experimental model and the length of time of administration. Estrogen dose varied according to the study (25 μg/kg × 7 days [ 10 ], 40–500 μg/kg × 14 days [ 11 ], 100 μg/kg × single dose [ 35 ] and 500 μg × 3 days [ 36 ]); however, no prior study has evaluated the effect of estrogen administration after reperfusion, either whole-body ischemia/reperfusion or renal vascular occlusion. In the present study, only higher dose of estrogen (400 μg/kg) injected during the early reperfusion phase improved the renal outcome in male mice.…”

Section: Discussionmentioning

confidence: 99%

Estrogen administered after cardiac arrest and cardiopulmonary resuscitation ameliorates acute kidney injury in a sex- and age-specific manner

et al. 2015

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Introduction: There is a sex difference in the risk of ischemic acute kidney injury (AKI), and estrogen mediates the protective effect of female sex. We previously demonstrated that preprocedural chronic restoration of physiologic estrogen to ovariectomized female mice ameliorated AKI after cardiac arrest and cardiopulmonary resuscitation (CA/CPR). In the present study, we hypothesized that male mice and aged female mice would benefit from estrogen administration after CA/CPR. We tested the effect of estrogen in a clinically relevant manner by administrating it after CA/CPR. Methods: CA/CPR was performed in young (10-15 weeks), middle-aged (43-48 weeks), and aged (78-87 weeks) C57BL/6 male and female mice. Mice received intravenous 17β-estradiol or vehicle 15 min after resuscitation. Serum chemistries and unbiased stereological assessment of renal injury were completed 24 h after CA. Regional renal cortical blood flow was measured by a laser Doppler, and renal levels of estrogen receptor alpha (ERα) and G protein-coupled estrogen receptor (GPER) were evaluated with immunoblotting. Results: Post-arrest estrogen administration reduced injury in young males without significant changes in renal blood flow (percentage reduction compared with vehicle: serum urea nitrogen, 30 %; serum creatinine (sCr), 41 %; volume of necrotic tubules (VNT), 31 %; P < 0.05). In contrast, estrogen did not affect any outcomes in young females. In aged mice, estrogen significantly reduced sCr (80 %) and VNT (73 %) in males and VNT (51 %) in females. Serum estrogen levels in aged female mice after CA/CPR were the same as levels in male mice. With age, renal ERα was upregulated in females. Conclusions: Estrogen administration after resuscitation from CA ameliorates renal injury in young males and aged mice in both sexes. Because injury was small, young females were not affected. The protective effect of exogenous estrogen may be detectable with loss of endogenous estrogen in aged females and could be mediated by differences in renal ERs. Post-arrest estrogen administration is renoprotective in a sex-and age-dependent manner.

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“…These observations were in agreement with others. [ 15 16 17 18 ] There are some reports suggesting that ET-1 is involved in the development of postischemic ARF in clinical transplantation. [ 19 20 ]…”

Section: Discussionmentioning

confidence: 99%

“…[ 51 ] Previous studies have demonstrated that testosterone has an important role in increasing the susceptibility to ischemic renal injury. [ 52 53 ] By contrast, other experimental results suggest that estrogen has a protective effect in ischemic renal injury in female[ 18 ] via suppression of ET-1 production and activation of the phopsphatidylinositol-3 kinase/protein kinase B signaling pathway. [ 54 55 ] Our result showed no difference in nitrite level between the male and female groups, and kidney MDA level reduced only in male, but other study showed that kidney MDA level decreased in male and female ischemic animals,[ 17 ] and IR decreased serum and kidney level of nitrite.…”

Section: Discussionmentioning

confidence: 99%

Preventive role of endothelin antagonist on kidney ischemia: Reperfusion injury in male and female rats

et al. 2015

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Background:Renal ischemia/reperfusion injury (RIRI) is the most common cause of acute kidney injury. We tested the protective role of endothelin-1 receptor blocker; bosentan (BOS) in animal model of RIRI in two different genders.Methods:Male and female Wistar rats were assigned as sham operated (sham), control group (ischemia), and case group (ischemia + BOS) treated with BOS (50 mg/kg) 2 h before bilateral kidney ischemia induced by clamping renal vessels for 45 min followed by 24 h of renal reperfusion.Results:The RIRI significantly increased the serum levels of blood urea nitrogen and creatinine in both genders (P < 0.05). These values were significantly decreased by BOS in both genders. In male rats, the serum levels of malondialdehyde in the ischemia + BOS group were decreased significantly when compared with ischemia group (P < 0.05).Conclusions:BOS can be used in both genders to attenuate kidney ischemia injury possibly due to its effect in the renal vascular system.

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Preventive role of estradiol on kidney injury induced by renal Ischemia-Reperfusion in male and female rats

Cited by 26 publications

References 39 publications

Gender differences in response to vitamin E and C in gentamicin induced nephrotoxicity in Wistar rats

Gender differences in response to vitamin E and C in gentamicin induced nephrotoxicity in Wistar rats

Estrogen administered after cardiac arrest and cardiopulmonary resuscitation ameliorates acute kidney injury in a sex- and age-specific manner

Preventive role of endothelin antagonist on kidney ischemia: Reperfusion injury in male and female rats

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