Preventive Effects of Three Polysaccharides on the Oxidative Stress Induced by Acrylamide in a Saccharomyces cerevisiae Model

Abstract

Saccharomyces cerevisiae was used as a model to explore the preventive effect of two marine polysaccharides separately derived from Sepia esculenta ink (SIP) and Laminaria japonica (FL) as well as one terrestrial polysaccharides from Eleocharis tuberosa peel (WCPP) on toxic injury induced by acrylamide (AA). The growth of yeast was evaluated by kinetics indexes including doubling time, lag phase and maximum proliferation density. Meanwhile, intracellular redox state was determined by contents of MDA and GSH, and SOD activity. The results showed that AA inhibited yeast growth and destroyed the antioxidant defense system. Supplement with polysaccharides, the oxidative damage of cells was alleviated. According to the growth recovery of yeast, FL and WCPP had similar degree of capacity against AA associated cytotoxicity, while SIP was 1.5~2 folds as strong as FL and WCPP. SIP and FL significantly reduced production of MDA by AA administration. Moreover, SIP, FL and WCPP increased SOD activity and repressed GSH depletion caused by AA.

Eleocharis dulcis corm: phytochemicals, health benefits, processing and food products

“…It is therefore essential to replenish antioxidants for the purpose of guarding against illnesses and aging. The antioxidant potentials of extracts, phenolics and polysaccharides from E. dulcis corm pulp and peel have been confirmed by different in vitro models 13,70,82,83 . For instance, ethanol extract of E. dulcis corm pulp shows stronger scavenging capacities against superoxide anions, 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) and hydroxyl radicals than vitamin C (VC) and butylated hydroxytoluene (BHT), two well‐known commercial antioxidants.…”

“…For other extracts, such as water extract, non-starch polysaccharides could also contribute to antioxidation. 19,83 On the other hand, Ren et al found that aureusidin (compound 53), a flavonoid that had been isolated from E. dulcis corm peel, elicits antiinflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages via the reactive oxygen species (ROS)dependent mitogen-activated protein kinase (MAPK) pathway, and unveiled that heme oxygenase-1 (HO-1), a key antioxidase that can protect cells from oxidative stress-induced damage, is involved in the anti-inflammatory process of aureusidin. 69,86,90 However, the above-mentioned activities of tested samples measured by in vitro assays would not accurately reflect the real status in vivo.…”

“…For other extracts, such as water extract, non‐starch polysaccharides could also contribute to antioxidation 19,83 . On the other hand, Ren et al .…”

“…The antioxidant potentials of extracts, phenolics and polysaccharides from E. dulcis corm pulp and peel have been confirmed by different in vitro models. 13,70,82,83 For instance, ethanol extract of E. dulcis corm pulp shows stronger scavenging capacities against superoxide anions, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals than vitamin C (VC) and butylated hydroxytoluene (BHT), two well-known commercial antioxidants. The higher antioxidant activities may be attributed to three main phenolicsi.e., gallocatechin gallate (compound 1), epicatechin gallate (compound 2) and catechin gallate (compound 3)total contents of these phenolics account for over 60% of all the phenolics present in E. dulcis corm pulp.…”

Complex Coronary Instent Chronic Total Occlusion Lesions: Oxidative Stress, Inflammation, and Coronary Stent Lengths

“…MDA production is formed after the breakdown of lipid hydroperoxides and is a biomarker of free radical injury in pathologies associated with free radical and oxidative stress. The levels of MDA as a marker of the oxidative stress response are considered as a sign of cell injury and promote the oxidant/antioxidant imbalance in cells [31,32]. hs-CRP as an inflammatory marker promotes acute and chronic inflammatory responses and is related to an adverse prognosis.…”