Preventive effect of D-carvone during DMBA induced mouse skin tumorigenesis by modulating xenobiotic metabolism and induction of apoptotic events

Gopalakrishnan, Thamizharasi; Sindhu, Ganapathy; Veeravarmal, Veeran; Nalini, Namasivayam

doi:10.1016/j.biopha.2018.12.071

Cited by 44 publications

(23 citation statements)

References 45 publications

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“…Therefore, antioxidants which are natural anti-inflammatory agents with least or no side effects are being studied to treat osteoarthritic conditions [ 17 ]. D-carvone was proven with multiple therapeutic potentials including anti-inflammatory effects [ 11 , 12 ]. The results from this research showed D-carvone was able to protect against the osteoarthritis induced by CFA in rats.…”

Section: Discussionmentioning

confidence: 99%

“…CFA-induced osteoarthritic model rats also exhibited liver damage as evidenced by the serum biochemical results with increased AST, ALT, and ALP levels which can also be attributed to the oxidative stress condition. The therapeutic dose of D-carvone oral administration in modulation of skin tumorigenesis and colon cancer in rat models was at 20 mg/kg and 10 mg/kg respectively [ 11 , 12 ]. A recent study on ameliorative effect of oral administration of D-carvone on lung inflammation in mice model was proven effective at the dose of 50 mg/kg [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

“…Given the involvement of oxidative stress and inflammatory response in the pathogenesis of osteoarthritis, importance was specified to plant-derived antioxidant compounds as the potential solution. D-carvone is an isomer of the monoterpene compound carvone, found in several aromatic plants, which has been reported to possess therapeutic effects such as immunomodulatory, anti-inflammatory, anti-nociceptive, anti-tumorigenic, anti-carcinogenic, anti-hyperlipidemic, anti-hypertensive, and chemopreventive on animal models and cell cultures [ 11 - 14 ]. To our knowledge, there are no reports on the anti-arthritic activity of D-carvone on animal or human model of osteoarthritis till date.…”

Section: Introductionmentioning

confidence: 99%

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Anti-arthritic activity of D-carvone against complete Freund’s adjuvant-induced arthritis in rats through modulation of inflammatory cytokines

Chen

Song

et al. 2020

Korean J Physiol Pharmacol

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Chronic joint pain due to loss of cartilage function, degradation of subchondral bone, and related conditions are common plights of an arthritis patient. Antioxidant compounds could solve the problems in arthritic condition. The objective of this study was to evaluate the anti-arthritic activity of D-carvone against complete Freund’s adjuvant (CFA)-induced arthritis in rats. D-carvone was orally administered for 25 days at the doses of 30 and 60 mg/kg against CFA-induced arthritic rats. Changes in body weight, paw swelling, organ index, hematological parameters, oxidative stress markers, inflammatory cytokines, and histopathology were recorded. Oral treatment of D-carvone significantly improved the body weight, reduced the paw swelling, edema formation, and organ index in arthritic rats. The levels of white blood cells were reduced, red blood cells and hemoglobin levels were improved in D-carvone treated arthritic rats. Lipid peroxidation levels were lowered whereas enzymatic and non-enzymatic antioxidants were significantly elevated by D-carvone administration against arthritic rats. D-carvone significantly modulated inflammatory cytokine levels and improved the ankle joint pathology against CFA-induced arthritic inflammation. In conclusion, D-carvone proved significant anti-arthritic activity against CFA-induced arthritis in rats.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anti-arthritic activity of D-carvone against complete Freund’s adjuvant-induced arthritis in rats through modulation of inflammatory cytokines

Chen

Song

et al. 2020

Korean J Physiol Pharmacol

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“…Mouse skin tumorigenesis model is the best recognized in vivo models for the study of the chronological and stepwise development of tumors from initiation to progression stage [13]. To date, this model is still being a model of choice to study the antitumor promoting effect of either synthetic or plant-based antineoplastic agents [14–16]. This due to the capability of his model mimics the features of multi-stage carcinogenesis in humans as humans are typically exposed to various doses of both carcinogens and promoting agents [17, 24].…”

Section: Discussionmentioning

confidence: 99%

Modulation of cancer signalling pathway(s) in two -stage mouse skin tumorigenesis by annonacin

Roduan

Hamid

Mohtarrudin

2019

BMC Complement Altern Med

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Background: Annonacin, an annonaceous acetogenin isolated from Annona muricata has been reported to be strongly cytotoxic against various cell lines, in vitro. Nevertheless, its effect against in vivo tumor promoting activity has not been reported yet. Therefore, this study was aimed to investigate antitumor-promoting activity of annonacin via in vivo two-stage mouse skin tumorigenesis model and its molecular pathways involved. Methods: Mice were initiated with single dose of 7,12-dimethylbenz[α]anthracene (DMBA) (390 nmol/100 μL) followed by, in subsequent week, repeated promotion (twice weekly; 22 weeks) with 12-O-tetradecanoylphorbol-13-acetate (TPA) (1.7 nmol/100 μL). Annonacin (85 nM) and curcumin (10 mg/kg; reference) were, respectively, applied topically to DMBA/ TPA-induced mice 30 min before each TPA application for 22 weeks. Upon termination, histopathological examination of skin, liver and kidney as well as genes and proteins expression analysis were conducted to elucidate the potential mechanism of annonacin. Results: With comparison to the carcinogen control, Annonacin significantly increased the tumor latency period and reduced the tumor incidence, tumor burden and tumor volume, respectively. In addition, it also suppressed tumorigenesis manifested by significant reduction of hyperkeratosis, dermal papillae and number of keratin pearls on skin tissues. Annonacin also appeared to be non-toxic to liver and kidney. Significant modulation of both AKT, ERK, mTOR, p38, PTEN and Src genes and proteins were also observed in annonacin-targeted signaling pathway(s) against tumorigenesis. Conclusions: Collectively, results of this study indicate that annonacin is a potential therapeutic compound targeting tumor promoting stage in skin tumorigenesis by modulating multiple gene and protein in cancer signaling pathways without apparent toxicity.

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“…7 ,12-dimethylbenz[a]anthracene (DMBA) is a carcinogenic chemical substance that used experimentally to induce a different variety of tumors in an attempt to study the pathogenesis of tumor and to assess the chemotherapeutic agents [1][2][3]. Experimentally, several studies showed the effectiveness of many substances in reducing or attenuating the effects of DMBA-induced tumorogenesis by improving the antioxidant status, augmenting the apoptosis, inducing antiproliferative, and activation of cytotoxic T cell-dependent tumor regression [4][5][6][7]. There are several biomarkers that contribute or related to the oral cancer.…”

Section: Introductionmentioning

confidence: 99%

Concomitant using of topical carrageenan-kappa and oral vitamin D against 7, 12-dimethylbenz[a]anthracene induced-oral cancer in rats: A synergism or an antagonism effects

Ali

Al-Juboori

Al-Nimer

2020

BDS

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Objective: κ-carrageenan is a food stabilizer agent which has an antiproliferative effect, while vitamin D is a prohormone acts on the nuclear receptor and has a cytotoxic against cancer. This study aimed to show the synergistic effect of using topical κ-carrageenan and oral administration of the vitamin D on the 7, 12-dimethylbenz[a] anthracene (DMBA)-induced oral cancer. Material and Methods: fifty four male albino rats were randomly divided into seven groups: Acetone-treated served as control (Group I), vitamin D (5000UI)-treated (Group II), κ-carrageenan (1%)- treated (Group III), DMBA (0.5%)-treated (Group IV), Acetone, κ-carrageenan and DMBA were administered topically on both cheeks and palate, five times weekly for 12 weeks, while the vitamin D was administered orally twice weekly for 12 weeks. Groups V, VI, and VII were animals treated with vitamin D, κ-carrageenan, and both vitamin D and κ-carrageenan for 8 weeks after induction of oral cancer. At the end of the study, blood samples were obtained by cardiac puncture for determination of TNF-α and EGFR. Results: In the groups III and IV, serum EGFR showed significant low levels compared with Group I. In the Group VII, serum EGFR showed a significantly (p=0.014) low level compared with Group IV (614.3±69.7 pg/ml versus 882.4±45.6 pg/ml, respectively). Higher percentages of high levels of TNF-α were observed in the Groups VI and VII, while a lower percentage of EGFR was observed in the Group VI. Conclusion: both κ-carrageenan and vitamin D have antiproliferative effect against DMBA-inducing oral cancer by increasing the levels of TNF-α and suppressing the signaling pathway of EGFR. Concomitant using κ-carrageenan and vitamin D reduces the antiproliferative effect of each other. KeywordsOral cancer; 7, 12-dimethylbenz[a] anthracene; Vitamin D; κ-carrageenan; Epidermal growth factor receptor; Tumor necrosis factor-α.

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Preventive effect of D-carvone during DMBA induced mouse skin tumorigenesis by modulating xenobiotic metabolism and induction of apoptotic events

Cited by 44 publications

References 45 publications

Anti-arthritic activity of D-carvone against complete Freund’s adjuvant-induced arthritis in rats through modulation of inflammatory cytokines

Anti-arthritic activity of D-carvone against complete Freund’s adjuvant-induced arthritis in rats through modulation of inflammatory cytokines

Modulation of cancer signalling pathway(s) in two -stage mouse skin tumorigenesis by annonacin

Concomitant using of topical carrageenan-kappa and oral vitamin D against 7, 12-dimethylbenz[a]anthracene induced-oral cancer in rats: A synergism or an antagonism effects

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