Prevention of vocal fold scarring by local application of basic fibroblast growth factor in a rat vocal fold injury model

Suzuki, Ryo; Kawai, Yoshitaka; Tsuji, Takuya; Hiwatashi, Nao; Kishimoto, Yo; Tateya, Ichiro; Nakamura, Tatsuo; Hirano, Shigeru

doi:10.1002/lary.26138

Cited by 32 publications

(40 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ideally, such biomaterial additives would induce local myofibroblasts to take on a “normal” VFF phenotype and promote pro‐inflammatory MΦ to alter their polarization toward an anti‐inflammatory/pro‐tissue healing phenotype. A growing body of literature indicates that basic fibroblast growth factor (bFGF) has direct anti‐fibrotic effects on VFF and indirect anti‐inflammatory effects . Specifically, VFF treated with bFGF have consistently shown reduced expression of α smooth muscle actin (a marker generally associated with a myofibroblast phenotype) and increased expression of MMP‐1 and hyaluronic acid synthase 2 (markers generally associated with an anti‐fibrotic phenotype) .…”

Section: Introductionmentioning

confidence: 99%

In vitroevaluation of a basic fibroblast growth factor‐containing hydrogel toward vocal fold lamina propria scar treatment

et al. 2017

View full text Add to dashboard Cite

Scarring of the vocal fold lamina propria can lead to debilitating voice disorders that can significantly impair quality of life. The reduced pliability of the scar tissue-which diminishes proper vocal fold vibratory efficiency-results in part from abnormal extracellular matrix (ECM) deposition by vocal fold fibroblasts (VFF) that have taken on a fibrotic phenotype. To address this issue, bioactive materials containing cytokines and/or growth factors may provide a platform to transition fibrotic VFF within the scarred tissue toward an anti-fibrotic phenotype, thereby improving the quality of ECM within the scar tissue. However, for such an approach to be most effective, the acute host response resulting from biomaterial insertion/injection likely also needs to be considered. The goal of the present work was to evaluate the anti-fibrotic and anti-inflammatory capacity of an injectable hydrogel containing tethered basic fibroblast growth factor (bFGF) in the dual context of scar and biomaterial-induced acute inflammation. An in vitro co-culture system was utilized containing both activated, fibrotic VFF and activated, pro-inflammatory macrophages (MΦ) within a 3D poly(ethylene glycol) diacrylate (PEGDA) hydrogel containing tethered bFGF. Following 72 h of culture, alterations in VFF and macrophage phenotype were evaluated relative to mono-culture and co-culture controls. In our co-culture system, bFGF reduced the production of fibrotic markers collagen type I, α smooth muscle actin, and biglycan by activated VFF and promoted wound-healing/anti-inflammatory marker expression in activated MΦ. Cumulatively, these data indicate that bFGF-containing hydrogels warrant further investigation for the treatment of vocal fold lamina propria scar. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1258-1267, 2018.

show abstract

Section: Introductionmentioning

confidence: 99%

In vitroevaluation of a basic fibroblast growth factor‐containing hydrogel toward vocal fold lamina propria scar treatment

et al. 2017

View full text Add to dashboard Cite

show abstract

“…They did not find any significant differences when comparing the treated group with the group receiving saline [15]. In another study, basic fibroblast growth factor was shown to reduce vocal cord scar formation [16]. Hirano et al found increased viscoelastic properties and less scar tissue formation after Hepatocyte Growth Factor injection [8].…”

Section: Discussionmentioning

confidence: 97%

Effects of phenytoin injection on vocal cord healing after mechanical trauma: An experimental study

et al. 2019

View full text Add to dashboard Cite

Background/aim: Phenytoin is an anticonvulsant drug which causes fibroblast proliferation, collagen synthesis, and an increase in epidermal growth factor. Therefore, the aim of the present study is to evaluate the effect of phenytoin injection on the wound healing process in rats with vocal cord injury by histopathological methods. Materials and methods: The vocal cords of 10 albino Wistar rats were damaged bilaterally; the left vocal cord was kept as the control group. Phenytoin was injected in the right vocal cord. Ten rats were sacrificed. The thickness of the lamina propria and density of the fibroblast and collagen were evaluated histopathologically. Results: Thickness of the lamina propria was 18.0 ± 7.1 µm in the control group, 65.5 ± 10.7 µm in the phenytoin group. The density of fibroblast and collagen were statistically lower in the control group compared the phenytoin group (P < 0.05). Conclusion: Phenytoin injection in rats after vocal cord injury significantly increased the thickness of the lamina propria and density of fibroblast and regular and mature collagen in the lamina propria. The findings in our study provide a feasible scientific view for adding phenytoin treatment to vocal cord surgeries in otolaryngology practice, but further studies are needed in order to evaluate the use of phenytoin in preventing the formation of scar tissue and possible effects on vocal cord vibration in humans after vocal cord injury.

show abstract

“…The use of bFGF to improve vocal fold scarring has been thoroughly investigated 333435363738. The first injection into an atrophic human vocal fold was described in 2008 34.…”

Section: Regenerative Medicine Advances In Oto-hnsmentioning

confidence: 99%

Advances in regenerative medicine for otolaryngology/head and neck surgery

McPhail

Janus

Lott

2020

BMJ

View full text Add to dashboard Cite

Head and neck structures govern the vital functions of breathing and swallowing. Additionally, these structures facilitate our sense of self through vocal communication, hearing, facial animation, and physical appearance. Loss of these functions can lead to loss of life or greatly affect quality of life. Regenerative medicine is a rapidly developing field that aims to repair or replace damaged cells, tissues, and organs. Although the field is largely in its nascence, regenerative medicine holds promise for improving on conventional treatments for head and neck disorders or providing therapies where no current standard exists. This review presents milestones in the research of regenerative medicine in head and neck surgery.

show abstract

Prevention of vocal fold scarring by local application of basic fibroblast growth factor in a rat vocal fold injury model

Abstract: N/A. Laryngoscope, 2016 127:E67-E74, 2017.

Cited by 32 publications

References 34 publications

In vitroevaluation of a basic fibroblast growth factor‐containing hydrogel toward vocal fold lamina propria scar treatment

In vitroevaluation of a basic fibroblast growth factor‐containing hydrogel toward vocal fold lamina propria scar treatment

Effects of phenytoin injection on vocal cord healing after mechanical trauma: An experimental study

Advances in regenerative medicine for otolaryngology/head and neck surgery

Contact Info

Product

Resources

About