Prevention of Selenite-Induced Cataractogenesis by N-Acetylcysteine in Rats

Aydın, Bahri; Yağcı, Ramazan; Yılmaz, Fatma Meriç; Erdurmuş, Mesut; Karadağ, Remzi; Keskın, Uğurcan; Durmuş, Mustafa; Yiğitoğlu, Ramazan

doi:10.1080/02713680802676885

Cited by 33 publications

(24 citation statements)

References 20 publications

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“…GSH protects the lens from oxidative damage and also maintains the functions and transparency of the lens by protecting the protein sulfhydryl groups from oxidation. In addition, GSH has been found to decrease concomitantly with seleniteinduced cataractogenesis [19][20][21][22][23][24][25]36].…”

Section: Discussionmentioning

confidence: 99%

“…The reliability and extensive characterization of selenite cataract makes it a useful rodent model for rapid screening of potential anticataract agents [16]. Molecules that have been shown by experimental studies to prevent selenite-induced cataractogenesis include melatonin [15], resveratrol [17], acetyl-L-carnitine [18,19], ellagic acid [20], soya bean [21], L-cysteine [22], N-acetylcysteine [23], an extract of Pleurotus ostreatus [24], and onion juice [25]. However, there is a need to evaluate additional compounds for their anticataractogenic effects.…”

Section: Introductionmentioning

confidence: 99%

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Prevention of Selenite-Induced Cataractogenesis by an Ethanolic Extract of Cineraria maritima: An Experimental Evaluation of the Traditional Eye Medication

Anitha

Thangaraj

Thomas

et al. 2010

Biol Trace Elem Res

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In the present study, the antioxidant potential of an ethanolic extract of Cineraria maritima and its efficacy in preventing selenite-induced cataractogenesis were assessed in vitro and in vivo. In the in vitro phase of the study, lenses dissected out from the eyes of Wistar rats were incubated for 24 h at 37 °C in Dulbecco's modified Eagle medium (DMEM) alone (group I), in DMEM containing 100 μM of selenite only (group II), or in DMEM containing 100 μM of selenite and 300 μg/ml C. maritima extract added at the same time (group III). Gross morphological examination of the lenses revealed dense opacification in group II, minimal opacification in group III, and no opacification in group I lenses. The mean activities of the antioxidant enzymes catalase, glutathione peroxidase, and superoxide dismutase were significantly lower in group II than in group I or group III lenses, while malondialdehyde concentration was significantly higher in group II lenses than in group I and group III lenses. In the in vivo phase of the study, dense opacification of lenses was noted in all rat pups (100%) that had received a single subcutaneous injection of sodium selenite alone (19 μM/kg body weight) on postpartum day 10, whereas cataract formation occurred in only 33.3% of rat pups that had received selenite as well as an intraperitoneal injection of the extract of C. maritima (350 mg/kg body weight) for five consecutive days. These observations suggest that the ethanolic extract of C. maritima may prevent experimental selenite-induced cataractogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Prevention of Selenite-Induced Cataractogenesis by an Ethanolic Extract of Cineraria maritima: An Experimental Evaluation of the Traditional Eye Medication

Anitha

Thangaraj

Thomas

et al. 2010

Biol Trace Elem Res

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show abstract

“…Several studies have suggested that intake of antioxidant-rich foods may slow the progression of cataract. Therefore, to date, a number of antioxidant chemicals and drugs such as L-carnitine, acetyl-L-carnitine, lycopene, melatonin, resveratrol, α-lipoic acid, Ginkgo biloba, α-tocopherol, N-acetylcysteine, pyruvate and verapamil have been used to prevent experimental cataract in the lens (11,12,14,15,(18)(19)(20)(21)(22)(23)(24)(25). They were found to be effective in preventing cataract, but none have been implemented in clinical practice.…”

Section: Discussionmentioning

confidence: 99%

The Protective Effect of Curcumin on Ionizing Radiation-induced Cataractogenesis in Rats

Özgen¹,

Dökmeci²,

Akpolat³

et al. 2012

Balkan Med J

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“…Several compounds with antioxidant properties have been reported to prevent selenite-induced cataracts, such as resveratrol [12], saffron [13], ellagic acid [14], garlic [15], melatonin [16], drevogenin D [17], caffeine [18], ebselen [19], lycopene in vivo [20], N-acetyl-L-carnitine in vitro and in vivo [21–23], and Ocimum sanctum in vivo and ex vivo [24]. In fact, N-acetylcysteine (NAC), a glutathione (GSH) prodrug, has previously been tried in selenite-induced cataracts in vivo [25] and was shown to prevent oxidative damage to the lens, slowing down cataractogenesis.…”

Section: Introductionmentioning

confidence: 99%

Prevention and reversal of selenite-induced cataracts by N-acetylcysteine amide in Wistar rats

Maddirala¹,

Tobwala²,

Karacal³

et al. 2017

BMC Ophthalmol

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BackgroundThe present study sought to evaluate the efficacy of N-acetylcysteine amide (NACA) eye drops in reversing the cataract formation induced by sodium selenite in male Wistar rat pups.MethodsForty male Wistar rat pups were randomly divided into a control group, an N-acetylcysteine amide-only group, a sodium selenite-induced cataract group, and a NACA-treated sodium selenite-induced cataract group. Sodium selenite was injected intraperitoneally on postpartum day 10, whereas N-acetylcysteine amide was injected intraperitoneally on postpartum days 9, 11, and 13 in the respective groups. Cataracts were evaluated at the end of week 2 (postpartum day 14) when the rat pups opened their eyes. N-acetylcysteine amide eye drops were administered beginning on week 3 until the end of week 4 (postpartum days 15 to 30), and the rats were sacrificed at the end of week 4. Lenses were isolated and examined for oxidative stress parameters such as glutathione, lipid peroxidation, and calcium levels along with the glutathione reductase and thioltransferase enzyme activities. Casein zymography and Western blot of m-calpain were performed using the water soluble fraction of lens proteins.ResultsMorphological examination of the lenses in the NACA-treated group indicated that NACA was able to reverse the cataract grade. In addition, glutathione level, thioltransferase activity, m-calpain activity, and m-calpain level (as assessed by Western blot) were all significantly higher in the NACA-treated group than in the sodium selenite-induced cataract group. Furthermore, sodium selenite- injected rat pups had significantly higher levels of malondialdehyde, glutathione reductase enzyme activity, and calcium levels, which were reduced to control levels upon treatment with NACA.ConclusionsThe data suggest that NACA has the potential to significantly improve vision and decrease the burden of cataract-related loss of function. Prevention and reversal of cataract formation could have a global impact. Development of pharmacological agents like NACA may eventually prevent cataract formation in high-risk populations and may prevent progression of early-stage cataracts. This brings a paradigm shift from expensive surgical treatment of cataracts to relatively inexpensive prevention of vision loss.

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Prevention of Selenite-Induced Cataractogenesis by N-Acetylcysteine in Rats

Abstract: N-acetylcysteine appears to inhibit selenite-induced cataractogenesis in the rat model, and this seems to be caused by the prevention of oxidative damage.

Cited by 33 publications

References 20 publications

Prevention of Selenite-Induced Cataractogenesis by an Ethanolic Extract of Cineraria maritima: An Experimental Evaluation of the Traditional Eye Medication

Prevention of Selenite-Induced Cataractogenesis by an Ethanolic Extract of Cineraria maritima: An Experimental Evaluation of the Traditional Eye Medication

The Protective Effect of Curcumin on Ionizing Radiation-induced Cataractogenesis in Rats

Prevention and reversal of selenite-induced cataracts by N-acetylcysteine amide in Wistar rats

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