Prevention of renal damage by alpha tocopherol in ischemia and reperfusion models of rats

Abstract

Ischemia-reperfusion injury in rat kidneys most probably comes from oxidative stress, but the possible preventive effect of alpha-tocopherol (AT) treatment on this injury has not yet been established. Forty male Wistar rats were randomly divided into four groups. The left renal arteries of all rats except the controls were clamped to induce renal ischemia. The left kidneys of the rats in the ischemia group were removed following 40 min ischemia. The rats in the ischemia-reperfusion and ischemia-reperfusion-AT groups were treated similarly, but in these groups the renal arteries were re-perfused for 1 h following ischemia. The rats in the ischemia-reperfusion-AT group also received 10 mg/kg AT 3 h prior to ischemia. The specimens were examined histopathologically and ultrastructurally, and the tissue calcium levels were measured. Light microscope and ultrastructural examination showed that the greatest damage occurred in the ischemia-reperfusion group. The highest level of tissue calcium was also found in this group. In the ischemia-reperfusion-AT-treated group, less tissue damage and a lower tissue calcium concentration was found compared to both the ischemia and ischemia-reperfusion groups. Our results indicate that AT can reduce tissue damage after ischemia-reperfusion injury.

The Protective Effects of Melatonin and Vitamin E against Renal Ischemia-Reperfusion Injury in Rats

“…Melatonin was administered 3 h and 15 min. prior to the renal ischemia in previous experimental ischemia-reperfusion models, [10,11,13] whereas in our study, melatonin was given 72 h, 48 h, 24 h, and 30 min before ischemia. Thus, our study is again different by the dosage of antioxidants, time of administration, and parameters studied.…”

“…However, the IR+Melatonin group findings were better than IR+vitamin E group. Avunduk et al [13] reported that in renal ischemia-reperfusion injury, vitamin E treatment prevented mitochondrial damage. Our IR+ vitamin E group findings were similar to this study.…”

“…[12,13] The protective effects of vitamin E on kidney tissue including renal ischemia reperfusion injury have yielded that it reduces lipid peroxidation and positive effects to antioxidant system status. [1,14,15] In light of the above findings, the present study examined the protective effects of exogenous melatonin and vitamin E supplementation on renal function, antioxidant status, lipid peroxidation production, and histopathological and electron microscopic changes in renal ischemia-reperfusion injury.…”

Chelatable Fe (II) is generated in the rat kidneys exposed to ischemia and reperfusion, and a divalent metal chelator, 2, 2'-dipyridyl, attenuates the acute ischemia/reperfusion-injury of the kidneys: a histochemical study by the perfusion-Perls and -Turnbull methods

“…The present results, thus, provided an important basis for the preventive effect of various antioxidants or free radical scavenging agents (Avunduk et al, 2003;Sehiril et al, 2003;Ohkita et al, 2005 and others) and ironchelators (e.g. desferrioxamine, Pincemail et al, 2000) on acute renal failure caused by ischemia or ischemia/ reperfusion.…”

Inhaled Hydrogen Gas Therapy for Prevention of Renal Ischemia/Reperfusion Injury in Rat Model

“…13 Numerous studies suggest that ROS can cause direct cellular damage through protein oxidation and degradation, lipid peroxidation and DNA damage and lipid peroxidation is believed to be an important cause of the destruction and damage to cell structure and function. 4,[14][15][16][17] Oxidative stress-induced cell injury is not only mediated by increased ROS production, but also by a decreased antioxidant defense system. 14 SOD is a class of enzymes that prevents damage from ROS during normal conditions by converting superoxide into hydrogen peroxide.…”