Prevention of peritoneal adhesions by intraperitoneal administration of vitamin E and human amniotic membrane

Yetkın, Gürkan; Uludağ, Mehmet; Çıtgez, Bülent; Karakoc, Sinan; Polat, Nedim; Kabukçuoğlu, Fevziye

doi:10.1016/j.ijsu.2009.09.007

Cited by 37 publications

(45 citation statements)

References 32 publications

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“…Keleidari et al 19 evaluated the effect of vitamin C on postoperative peritoneal adhesions and reported better wound healing and decreased adhesion formation in the experimental group than in the control group. The efficacy of vitamin E in the prevention of post-operative peritoneal adhesion has been reported 1,20,21 . Şahbaz et al 2 demonstrated that pycnogenol, which is an antioxidant, was effective for preventing peritoneal adhesion on postoperative day 10 owing to its anti-inflammatory and antioxidant effects.…”

Section: ■ Discussionmentioning

confidence: 99%

The effect of pycnogenol on lymphatic nodes and adhesion during in a peritoneal adhesion model in rats

Göret¹,

Göret

Kiraz

et al. 2018

Acta Cir. Bras.

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Purpose: To investigate the effects of pycnogenol on peritoneal adhesions and additionally to investigate the immunohistochemical effects of free oxygen radicals and reactive lymph nodes detected in the adhesive tissue that was sampled surrounding the cecum on intraabdominal adhesions. Methods: Twenty-seven Wistar Albino rats were divided into three groups. In group 1 (sham), laparotomy was performed and stitched up. In group 2 (control), after laparotomy was performed, punctate hemorrhage was induced by cecal abrasion in the cecum and each rat was intraperitoneally administered 2 cc of saline. In group 3 (experimental), after laparotomy was performed, punctate hemorrhage was induced by cecal abrasion in the cecum and each rat was intraperitoneally administered a sterile Pycnogenol derivative. The rats in all groups were re-laparotomized on postoperative day 7; samples were obtained from the peritoneal tissue surrounding the cecum, and the rats were sacrificed. Results: In group 3, there was a statistically significant difference in terms of inflammation, lymph node size, and free oxygen radicals; these parameters tended to increase. In terms of fibrosis evaluated using H&E and MT, there was no significant difference between groups 2 and 3. Conclusions: No positive outcomes indicating that pycnogenol reduces intra-abdominal adhesions were obtained. However, it caused severe inflammation in the tissue. Moreover, a significant increase in lymph node size was detected secondary to inflammation. Additionally, in immunohistochemical analyses conducted to detect oxidative stress, pycnogenol increased the production of free oxygen radicals in the tissue.

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Section: ■ Discussionmentioning

confidence: 99%

The effect of pycnogenol on lymphatic nodes and adhesion during in a peritoneal adhesion model in rats

Göret¹,

Göret

Kiraz

et al. 2018

Acta Cir. Bras.

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“…Vitamin E presents interesting biological properties and activities for preventing intraperitoneal adhesions. In vitro studies have reported that it has antioxidant, anti-inflammatory, anticoagulant and antifibroblastic effects, and playing a role in diminishing the production of collagen [3][4][5] . In recent studies, it has been suggested that intraperitoneal application of MB can be used as an effective agent in the prevention of postoperative adhesions 6 .…”

Section: Introductionmentioning

confidence: 99%

The comparison of methylene blue and vitamin E in prevention of abdominal postoperative adhesion formation in rat uterine horn models: Biochemical and histopathologic evaluation

et al. 2011

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Comparação do azul de metileno e vitamina E na prevenção de aderência abdominal pós-operatória em corno uterino de ratos. Avalição bioquímica e histopatológica ABSTRACT Purpose:To compare the effects of vitamin E and 1% methylen blue solutions on prevention of experimentally induced adhesions in rats. Methods: Thirty seven female Spraque Dawley rats were randomized into four groups. First group was kept as sham operated group. An adhesion model was constituted on the left uterine horn of the other groups. The lesion areas of rats from the second, the third and the fourth groups were coated with 2 ml 0.9 % saline solution (C group), 10 mg vitamin E (VE group) and 1% methylen blue solutions (MB group), respectively. Results: Histopathologically, adhesion scores, mononuclear cell infiltration, oedema and fibrosis were more prominent in the MB group compared with C and VE groups. There were no significant differences between the groups in tissue glutathione peroxidase (GPx), catalase (CAT) activities and glutation (GSH) level, these parameters were slightly increased in group with VE supplementation though. The administration of VE and MB significantly decreased NO (P<0.01) levels when compared to the C group. The level of malondialdehyde (MDA) in the VE group was significantly lower (P<0.05) than those of the Sh and C groups. Conclusion: Intraperitoneal methylen blue solutions treatments were more effective according to vitamin E in preventing the formation of intra-abdominal adhesion in a rat uterine horn model. Key words: Vitamin E. Methylene Blue. Tissue Adhesions. Uterus. Rats. RESUMO Objetivo:Comparar os efeitos da vitamina E e 1% da solução de azul de metileno na prevenção de aderências induzidas em ratos. Métodos: Trinta e sete ratos fêmeas Spraque Dawley foram distribuídos em quatro grupos. O primeiro grupo foi mantido como grupo sham. O modelo de aderência foi realizado no corno uterino esquerdo nos outros grupos. As áreas da lesão dos ratos do segundo, terceiro e quarto grupos foram revestidas com 2 ml de solução salina 0,9% (Grupo C), 10 mg de vitamina E (Grupo VE) e solução de azul de metileno 1% (Grupo MB), respectivamente. Resultados: Histopatologicamente, o escore das aderências, infiltração celular mononuclear, edema e fibrose foram mais proeminentes no grupo MB em comparação aos grupos C e VE. Não houve diferença significante entre os grupos na peroxidase da glutatione do tecido (GPx), atividade da catalase (CAT) e o nível de glutation (GSH). Estes parâmetros foram ligeiramente aumentados no grupo com suplemento da VE. A administração da VE e do MB diminuiu significantemente os níveis quando quando comparada ao Grupo C. O nível de malondialdeído no grupo VE foi significantemente mais baixo do que nos grupos sham e C. Conclusão: A administração intraperitoneal da solução de azul de metileno foi mais eficaz de acordo com a vitamina E na prevenção de aderências intra-abdominais no corno uterino de ratos. Descritores: Vitamina E. Azul de Metileno. Aderências Teciduais. Útero. Ratos. Yildiz H et al 52

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“…The proposed mechanisms with these agents include reducing inflammation, increasing fibrinolysis capacity, preventing fibroplast proliferation, or separating the deperitonealized areas. Also, different antioxidant agents such as Vitamin C, Vitamin E, N-acetyl cysteine, and melatonin have been used to prevent adhesions [20,21] . Since oxytocin is known to have antiinflammatory and anti-oxidant effects, we tried to evaluate its impact on the prevention of adhesions.…”

Section: Discussionmentioning

confidence: 99%

Uterotonik Bir Ajandan Fazlası: Oksitosin Postoperatif Adezyonları Önlüyor

Işık¹,

Şahbaz²,

Aynıoğlu³

et al. 2016

Kafkas Univ Vet Fak Derg

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Prevention of postoperative adhesions (PPA) has become an important issue. The aim is to investigate the effect of Oxytocin (OT) on PPAs. A total of thirty female Wistar-albino rats were randomly divided into three groups (10 rats/group). The cecal peritone of Group I rats (controls) were scraped, to trigger adhesion formation, and no treatment were given. After cecal scrubbing, 1 mL saline solution was applied to each rat in Group II (i.p. saline treated group) and 80 IU/kg of OT (Pituisan®, Ege Vet, Turkey) to Group III (i.p. OT treated group) intraperitoneally. All animals were sacrificed 10 days after surgery and adhesions graded in terms of severity and histopathologic characteristics. The median scores for the extent, severity, and degree of adhesions in Group I and Group II were statistically significant and considerably higher than those scores for Group III (P<0.001). The inflammation, neovascularization, and fibrosis scores for Group III were statistically significant and considerably lower than those scores for Groups I and II (P<0.001, P<0.001 and P=0.002 respectively). OT, significantly prevented adhesion formation improving wound healing possibly by suppressing adhesion formation with anti-inflammatory and antioxidant properties. OT may be useful in the prevention of PPA in humans.

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Prevention of peritoneal adhesions by intraperitoneal administration of vitamin E and human amniotic membrane

Abstract: Intraperitoneal vitamin E and amniotic membrane treatment were both effective in the prevention of peritoneal adhesions. The combination of these agents did not produce a synergistic effect. Easy applicability of the intraperitoneal administration of vitamin E was its major advantage.

Cited by 37 publications

References 32 publications

The effect of pycnogenol on lymphatic nodes and adhesion during in a peritoneal adhesion model in rats

The effect of pycnogenol on lymphatic nodes and adhesion during in a peritoneal adhesion model in rats

The comparison of methylene blue and vitamin E in prevention of abdominal postoperative adhesion formation in rat uterine horn models: Biochemical and histopathologic evaluation

Uterotonik Bir Ajandan Fazlası: Oksitosin Postoperatif Adezyonları Önlüyor

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