Prevention of Cellular Oxidative Damage by an Aqueous Extract of Anoectochilus formosanus

Abstract

Anoectochilus formosanus (AF) is a popular folk medicine in Taiwan whose pharmacological effects have been characterized. In this work we investigated the antioxidant properties of an aqueous extract prepared from AF. The AF extract was capable of scavenging H2O2 in a dose-dependent manner. We induced oxidative stress in HL-60 cells, either by the addition of hydrogen peroxide (H2O2) or by the xanthine/xanthine oxidase reaction. Apoptosis caused by oxidative damage was displayed by DNA fragmentation on gel electrophoresis, and the apoptotic fraction was quantified with flow cytometry. The cell damage induced by oxidative stress was prevented by the plant extract in a concentration-dependent manner. Furthermore, the proteolytic cleavage of poly(ADP-ribose) polymerase during the apoptotic process was also inhibited by AF extract. Our results provide the basis for determining an AF extract to be an antioxidant.

Anti‐inflammatory and anti‐insulin resistance activities of aqueous extract from Anoectochilus burmannicus

“…They have remarkable curative effects of clearing heat and cooling blood, removing dampness, detoxification, antioxidative stress (Shih, Wu, & Lin, 2002; Wang et al., 2005), and anti‐inflammation (Hsieh, Hsiao, & Lin, 2010). Previous studies reported that kinsenoside isolated from Anoectochilus roxburghii and Anoectochilus formosan us provide beneficial effects on hepatoprotective (Wang et al., 2005), antihyperlipolysis activities (Du et al., 2001).…”

“…They have remarkable curative effects of clearing heat and cooling blood, removing dampness, detoxification, antioxidative stress (Shih, Wu, & Lin, 2002; Wang et al., 2005), and anti‐inflammation (Hsieh, Hsiao, & Lin, 2010). Previous studies reported that kinsenoside isolated from Anoectochilus roxburghii and Anoectochilus formosan us provide beneficial effects on hepatoprotective (Wang et al., 2005), antihyperlipolysis activities (Du et al., 2001). It also reduces blood glucose (Zhang, Cai, Ruan, Pi, & Wu, 2007; Zhang, Liu, Liu, Li, & Yi, 2015), modulaties the allergic response, and exerts anti‐inflammation in both the LPS‐induced inflammation in macrophages and endotoxin‐shocked mice (Hsiao, Wu, Lin, & Lin, 2011).…”

“…Several Thai traditional plants such as ginger (Grzanna, Lindmark, & Frondoza, 2005), longan (Ho, Hwang, Shen, & Lin, 2007;Wang, Tang, Chiu, & Huang, 2012), and Gymnema inodorum (Shimizu et al, 2001) (Yang, Wu, Lu, & Lin, 2013). They have remarkable curative effects of clearing heat and cooling blood, removing dampness, detoxification, antioxidative stress (Shih, Wu, & Lin, 2002;Wang et al, 2005), and anti-inflammation (Hsieh, Hsiao, & Lin, 2010). Previous studies reported that kinsenoside isolated from Anoectochilus roxburghii and Anoectochilus formosanus provide beneficial effects on hepatoprotective (Wang et al, 2005), antihyperlipolysis activities (Du et al, 2001).…”

Further studies on the hepatoprotective effects of Anoectochilus formosanus

“…These reports include experimental acute hepatitis in rats induced by CCl 4 or acetaminophen (Lin et al, 1993;2000), chronic hepatitis in rats caused by CCl 4 or dimethylnitrosamine (Shih et al, 2004;, and CCl 4 or H 2 O 2 induced cytotoxicity in cultured hepatocytes (Du et al, 2003;Wu et al, 2007). Numerous studies have indicated that A. formosanus extracts are good free radical scavengers (Wang et al, 2002;Shih et al, 2003;Wang et al, 2005), suggesting that the ameliorative effects of A. formosanus on liver injury induced by hepatoxin are due, at least in part, to its free-radical scavenging ability. In the present study, RT-PCR was used to analyse the mRNA expressions of MAT1A, collagen (α1)(I) and TGF-β1.…”

Molecular Cloning of a New Immunomodulatory Protein from Anoectochilus formosanus which Induces B Cell IgM Secretion through a T-Independent Mechanism

“…Anoectochilus formosanus is an herbal plant widely used as dietary supplement and folk remedy in Asia. It provided numerous health benefits such as hepatoprotection [1] – [4] , anti-fatigue [5] , anti-oxidative [6] , [7] , anti-hyperglycemia [8] , anti-hyperliposis [4] , anti-osteoporosis [9] , [10] , anti-tumor [11] , [12] , and immune modulation. The aqueous extraction of A. formosanus was reported to stimulate lymphoid tissue and peritoneal macrophages [11] .…”