Prevention of Atopic Dermatitis in Mice by Lactobacillus Reuteri Fn041 Through Induction of Regulatory T Cells and Modulation of the Gut Microbiota

Abstract

Scope: The development of atopic dermatitis (AD) in infants is closely related to the lagging development of intestinal microbiota, including that inoculated by breast milk bacteria, and immune development. Lactobacillus reuteri Fn041 is a secretory immunoglobulin A (sIgA) -coated bacterium derived from human milk. Methods and Results: We intervene with L. reuteri Fn041 in maternal and offspring BALB/C mice during late gestation and lactation and after weaning of the pups, respectively. AD is then induced with MC903. L. reuteri Fn041 significantly suppresses AD symptoms such as skin swelling, mast cell, and eosinophil infiltration. This effect is attributed to the regulation of the systemic Th1 and Th2 cytokine ratios and the promotion of CD4 + CD25 + Foxp3 + regulatory T cell proliferation in mesenteric lymph nodes. It is also associated with the regulation of intestinal microbiota, particularly promoting Lactobacillus and Akkermansia. Conclusion: Our study strengthens the understanding that breast milk-derived sIgA coated potential probiotics are involved in the development of infant intestinal microbiota, thus promoting immune development and preventing allergic diseases, and expanding the knowledge of breast milk sIgA and bacterial interactions on infant immune development.

Gut microbiota and atopic dermatitis in children: a scoping review

“…These bacteria are affected by the mode of delivery, living and feeding environment. Anaerobic microorganisms start to multiply when breastfeeding begins to stimulate the baby's intestines [ 49 ]. Bifidobacterium, Lactobacillus and Bacteroides belong to this type of bacteria, because the oligosaccharides in breast milk stimulate the baby's stomach, so the most bacteria produced are probiotics.…”

Limosilactobacillus reuteri Attenuates Atopic Dermatitis via Changes in Gut Bacteria and Indole Derivatives from Tryptophan Metabolism

“…L. reuteri strain has the potential to alleviate AD symptoms via modulating immune responses. L. reuteri Fn041, a strain secreting IgA, significantly improved skin swelling and inflammatory cell infiltration via restoring the systemic Th1 and Th2 cytokine ratios and Treg proliferation [ 37 ]. Likewise, L. reuteri DYNDL22M62 variably affected AD-like symptoms, which could be shown based on H&E staining and changes in cytokines.…”

Mixed nuts with high nutrient density improve insulin resistance in mice by gut microbiota remodeling

“…According to the previous experimental method, 45 aqueous solutions of SCFAs were extracted from fecal samples and tested by a gas chromatography-mass spectrometer (Agilent 8890 GC system). Standards were diluted, extracted, and derivatized to obtain standard curves and to quantify the analytes.…”