Prevention of acute ruminal lactic acidosis in sheep by probiotic or monensin supplementation: clinical aspects

Abstract

The aim of this study was to evaluate the effects of two additives (probiotic and monensin) over clinical parameters of sheep submitted to acute ruminal lactic acidosis (ARLA). Eighteen sheeps were divided into three groups of six animals each as follows: probiotic group, supplemented with 4×10 9 CFU/animal/ day of Saccharomyces cerevisiae; monensin group, supplemented with 33 mg of monensin sodium per kg of diet; and control group, without any supplementation. After 30 days of diet (75% of Coast-cross hay and 25% concentrate with 14% of crude protein) and additive intake, ARLA was induced in the all animals by intraruminal administration of 15g of sucrose per kilogram (kg) of body weight. Complete physical examinations to assess the vital signs of the animals were conducted at the following times: baseline (T0), six (T6h), 12 (T12h), 18 (T18h), 24 (T24h), 36 (T36h), and 48 (T48h) hours after ARLA induction. At those times, blood samples were obtained to determine the plasma volume deficit (PVD) and ruminal content for pH measurement. All animals experienced clinical signs of ARLA with ruminal pH lower than 4.9 at T24h. The heart rate and PVD were lower (P < 0.05) in the probiotic group at T36h. Compared to the control and monensin groups, the probiotic group experienced milder ARLA characterized by lower degrees of dehydration and fewer clinical symptoms.Objetivou-se avaliar os efeitos de dois aditivos (probiótico e monensina sódica) sobre a parâmetros clínicos de ovinos submetidos a acidose láctica ruminal aguda (ALRA). Dezoito ovinos foram divididos em três grupos com seis animais cada assim constituídos: Probiótico, suplementado com 4x10 9 ufc/ animal/dia de Saccharomyces cerevisiae; Monensina, suplementado com 33 mg/kg de monensina sódica; Controle, sem aditivo. Após 30 dias de oferecimento da dieta (75% feno de Coast-cross e 25% concentrado com 14% de proteína bruta) e aditivos, os animais foram submetidos à indução de ALRA por meio da administração intraruminal de 15 gramas de sacarose por kg de peso vivo. Foi realizado exame físico completo nos animais com aferição dos parâmetros vitais nos seguintes momentos: basal (T0), seis (T6h), doze (T12h), 18 (T18h), 24 (T24h), 36 (T36h) e 48 (T48h) horas após a indução da ALRA. Em todos os momentos de avaliação foram obtidas amostras de sangue para determinação do déficit de volume plasmático (DVP) e de conteúdo ruminal para aferição do pH. Todos os animais apresentaram quadro de ALRA com pH ruminal menor que 4,9 no T24h, sem diferença entre os grupos (p = 0.092). A frequência cardíaca e o DVP foram inferiores (P<0,05) no grupo Probiótico no T36h. Os animais do grupo Probiótico apresentaram quadro de ALRA mais branda observada por menor grau de desidratação e menor ocorrência de sintomas clínicos em relação aos animais dos grupos controle e monensina. Palavras-chave: Aditivo. Ionóforo. Saccharomyces cerevisiae. Sintomas.

Cardiac biomarkers in goats with experimental ruminal lactic acidosis and supplemented with monensin sodium

“…The clinical observations in the goats of the two groups in this study was characteristic of RLA and the intensity of the clinical manifestations coincided with the decrease in ruminal pH, corroborating with other authors who evaluated the disease in this species under experimental conditions (CAO et al, 1987;MIRANDA NETO et al, 2005) and cases of natural occurrence (JOSHI et al, 2017). The majority of the animals in the two groups presented clinical reestablishment 48h after induction, differing from other authors who observed in sheep experimentally submitted to ruminal acidosis, early recovery in animals receiving monensin sodium in the diet, when compared to the control group, allowing the authors to infer that supplementation with ionophore contributed to faster recovery of the ruminal environment, reducing the severity of the clinical presentation (AFONSO et al, 2005;REIS et al, 2018a).…”

Ruminal Acidosis Part I: Clinical manifestations, epidemiology and impact of the disease

“…Behaviour alterations are man-ifested by anorexia, weakness, depression, lethargy, staggering, recumbency, flank watching, and lameness (Meyer and Bryant, 2017;Pupin et al, 2017). Clinical and laboratory testing reveal pyrexia in early stage and hypothermia in later stages, tachycardia, dehydration, congestion of mucous membranes and vessels of sclera, pneumonia, metabolic acidosis, hypocalcaemia, severe toxemia, acidified urine, and ruminal atony (Snyder and Credille, 2017;Reis et al, 2018).…”