Prevention and partial reversal of diabetes-induced changes in enteric nerves of the rat ileum by combined treatment with α-lipoic acid and evening primrose oil

Shotton, Hannah; Broadbent, Steven; Lincoln, J.

doi:10.1016/j.autneu.2004.02.004

Cited by 35 publications

(26 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An incremental increase in vasoactive intestinal polypeptide and nitric oxide synthase (NOS) content in the myenteric plexus is observed after 8 weeks experimentally induced DM (Shotton et al 2007). Neuronal subpopulations also react differentially to various diabetic neuropathy treatments (Shotton et al 2003(Shotton et al , 2004. New treatment strategies have been shown to be effective against neurovascular dysfunction induced by diabetes.…”

Section: Introductionmentioning

confidence: 98%

Vitamin E supplementation in rats with experimental diabetes mellitus: analysis of myosin-V and nNOS immunoreactive myenteric neurons from terminal ileum

et al. 2008

View full text Add to dashboard Cite

The effect of vitamin E (1 g/kg body weight) supplementation on myosin-V and neuronal nitric oxide synthase (nNOS) immunoreactive myenteric neurons from the ileum of diabetic rats was investigated in the present study. Forty animals were divided into the following groups: normoglycemics (N), normoglycemics treated with vitamin E (NE), diabetics (D), and diabetics treated with vitamin E (DE). Quantitative and morphometric analyses were performed. The area of the tertiary plexus was also determined. Diabetes produced a 24% reduction in the number of myosin-V neurons in group D compared with group N, an effect that was accompanied by an increase in the tertiary plexus area (P < 0.05). Neuronal density was 27% higher in group NE than group N (P < 0.05). Nitrergic neuronal density was not altered as a consequence of either diabetes or vitamin E treatment. Myosin-V and nNOS immunoreactive neuronal cell body area increased significantly in group NE. The area of myosin-V and nNOS myenteric neurons also increased in group D. Vitamin E treatment (group DE) increased only the size of nitrergic neurons. The present results suggest that vitamin E elicited a neuroprotective and neurotrophic effect on the natural aging process, but with regard to diabetes, vitamin E supplementation exerted a neurotrophic effect only on nitrergic neurons.

show abstract

Section: Introductionmentioning

confidence: 98%

Vitamin E supplementation in rats with experimental diabetes mellitus: analysis of myosin-V and nNOS immunoreactive myenteric neurons from terminal ileum

et al. 2008

View full text Add to dashboard Cite

show abstract

“…11) Therapeutic approaches using ALA with deˆnite eŠects in both the prevention and treatment of diabetes-induced oxidative stress have been reported in rat kidney 12) and enteric nerves of the rat ileum. 13) To our knowledge, however, there is no information available of eŠects of ALA on impaired vascular reactivity of diabetic rats. The purpose of this study was to investigate the eŠects of ALA treatment on vascular reactivity in streptozotocin (STZ)-induced diabetic rats.…”

Section: Introductionmentioning

confidence: 99%

Alpha Lipoic Acid Treatment Improved Endothelium-dependent Relaxation in Diabetic Rat Aorta

et al. 2011

View full text Add to dashboard Cite

The aim of this study was to ascertain the eŠects of a-lipoic acid (ALA) treatment on relaxant responses of acetylcholine (ACh) and isoprenaline (ISO) in aortic rings precontracted with serotonin (5-HT, 10 -6 M) obtained from streptozotocin (STZ)-induced diabetic rats. Diabetes was induced in the rats by 50 mg/kg streptozotocin (STZ) via an intraperitoneal injection. Rat body and aorta weights were measured. The isometric tension to ACh (10 -9  3×10 -6 M) and ISO (10 -9  10 -4 M) of 5-HT-precontracted diabetic and non-diabetic rat (control), diabetic-ALA-treated, and ALA-treated aortas, in organ baths were recorded. Six weeks after STZ treatment blood glucose was elevated compared to control rats. In aortic rings from diabetic rats ACh and ISO-induced relaxations were impaired whereas endotheliumindependent relaxation to sodium nitroprusside (SNP) was unaŠected. ALA (100 mg/kg/day) treatment for 5 weeks enhanced ACh and ISO-induced relaxation in diabetic aortas. This recovering eŠect was via NO because prevented by incubating the vessels with N G -nitro-L-arginine methyl ester (L-NAME, a NOS inhibitor). It may be assumed that ALA treatment in vivo, can protect against impaired vascular responsiveness in STZ-induced diabetic rats.

show abstract

“…A possible explanation for the increase in both the density and proportion of NADHd-positive neurons observed in groups D and T is offered by authors who attribute the increase in the NADH/NAD+ ratio to the high plasma glucose levels [37,38] and its vascular [13] and metabolic consequences, which influence the activity of enzymes using those cofactors [39]. In this way, acute diabetes, that, at the same time, leads to the loss of a fraction of the total population of neurons (HuC/HuD neurons), would heighten the activity of the respiratory chain and its enzymes, resulting in a greater availability of electrons to be transferred to nitro blue tetrazolium, which turns into formazan granules when acting as an artificial electron acceptor.…”

Section: Discussionmentioning

confidence: 98%

“…The neuronal subpopulations are affected differently by diabetes according to the intestinal segment [8][9][10], as well as the distinct methods of diabetes treatment and duration of the illness [11][12][13][14][15][16]. Previous studies have shown a reduction in the number of enteric neurons [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Effects of Insulin Treatment on HuC/HuD, NADH Diaphorase, and nNOS-Positive Myoenteric Neurons of the Duodenum of Adult Rats with Acute Diabetes

et al. 2008

View full text Add to dashboard Cite

We carried out this investigation with the purpose of verifying whether insulin treatment prevents changes in the density of myoenteric neurons of the duodenum of Wistar rats with streptozotocin short-term diabetes. The animals from the diabetic group (D) lost more weight than the controls (group C), while the insulin treatment (group T) prevented weight loss in three animals and increased visceral fat in all of the animals of this group. Insulin treatment did not prevent the early loss of HuC/HuD myoenteric neurons. The density of nNOS-positive neurons did not change significantly in groups D and T. The density of NADHd-positive neurons in these groups was greater than in group C, indicating that short-term diabetes increases the activity of respiratory chain enzymes.

show abstract

Prevention and partial reversal of diabetes-induced changes in enteric nerves of the rat ileum by combined treatment with α-lipoic acid and evening primrose oil

Cited by 35 publications

References 28 publications

Vitamin E supplementation in rats with experimental diabetes mellitus: analysis of myosin-V and nNOS immunoreactive myenteric neurons from terminal ileum

Vitamin E supplementation in rats with experimental diabetes mellitus: analysis of myosin-V and nNOS immunoreactive myenteric neurons from terminal ileum

Alpha Lipoic Acid Treatment Improved Endothelium-dependent Relaxation in Diabetic Rat Aorta

Effects of Insulin Treatment on HuC/HuD, NADH Diaphorase, and nNOS-Positive Myoenteric Neurons of the Duodenum of Adult Rats with Acute Diabetes

Contact Info

Product

Resources

About