Preventing root caries development under oral biofilm challenge in an artificial mouth

Mei, May Lei; Chu, Chun Hung; Lo, Edward-Chin-Man; Samaranayake, Lakshman-Perera

doi:10.4317/medoral.18768

Cited by 14 publications

(7 citation statements)

References 33 publications

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“…In caries research, there is a large variety of in vitro biofilm models, using different bacterial growth technologies, such as cell/tissue culture plates [ 32 , 33 , 34 , 35 , 36 , 37 ], multi-plaque artificial mouth (MAM) [ 38 ], constant depth film fermenter (CDFF) [ 39 ], chemostats [ 40 ], center for disease control (CDC) biofilm reactor [ 41 ], flow chambers and flow cell [ 42 , 43 , 44 ] (e.g., ten Cate´s Model [ 44 ]), artificial mouth computer controlled (AMCC) [ 45 , 46 , 47 ] or the multi-station continuous-culture biofilm model (MSCBM) [ 48 ].…”

Section: Oral Biofilms and In Vitro Biofilm Modelsmentioning

confidence: 99%

Relevance of Biofilm Models in Periodontal Research: From Static to Dynamic Systems

et al. 2021

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Microbial biofilm modeling has improved in sophistication and scope, although only a limited number of standardized protocols are available. This review presents an example of a biofilm model, along with its evolution and application in studying periodontal and peri-implant diseases. In 2011, the ETEP (Etiology and Therapy of Periodontal and Peri-Implant Diseases) research group at the University Complutense of Madrid developed an in vitro biofilm static model using representative bacteria from the subgingival microbiota, demonstrating a pattern of bacterial colonization and maturation similar to in vivo subgingival biofilms. When the model and its methodology were standardized, the ETEP research group employed the validated in vitro biofilm model for testing in different applications. The evolution of this model is described in this manuscript, from the mere observation of biofilm growth and maturation on static models on hydroxyapatite or titanium discs, to the evaluation of the impact of dental implant surface composition and micro-structure using the dynamic biofilm model. This evolution was based on reproducing the ideal microenvironmental conditions for bacterial growth within a bioreactor and reaching the target surfaces using the fluid dynamics mimicking the salivary flow. The development of this relevant biofilm model has become a powerful tool to study the essential processes that regulate the formation and maturation of these important microbial communities, as well as their behavior when exposed to different antimicrobial compounds.

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Section: Oral Biofilms and In Vitro Biofilm Modelsmentioning

confidence: 99%

Relevance of Biofilm Models in Periodontal Research: From Static to Dynamic Systems

et al. 2021

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“…The combination of silver with fluoride led to the formation of silver diamine fluoride (SDF), characterized by antibacterial properties, for which the metal component is responsible, and remineralization properties due to the presence of fluoride [ 85 ]. It has been shown that SDF can be used as an element of dental caries prevention [ 86 ], as well as a substance inhibiting the development of disease by reducing the amount of cariogenic microorganisms in the oral cavity and that it has a positive influence in zones of tooth enamel demineralization [ 85 ]. During studies on the described compound, the sensitivity of S. mutans and Actinomyces naeslundii to its function was also demonstrated [ 87 ].…”

Section: Inhibition Of Biofilm Formation—treatment Perspectives In Dementioning

confidence: 99%

The virulence of Streptococcus mutans and the ability to form biofilms

Krzyściak

Jurczak

Kościelniak

et al. 2013

Eur J Clin Microbiol Infect Dis

464

343

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In some diseases, a very important role is played by the ability of bacteria to form multi-dimensional complex structure known as biofilm. The most common disease of the oral cavity, known as dental caries, is a top leader. Streptococcus mutans, one of the many etiological factors of dental caries, is a microorganism which is able to acquire new properties allowing for the expression of pathogenicity determinants determining its virulence in specific environmental conditions. Through the mechanism of adhesion to a solid surface, S. mutans is capable of colonizing the oral cavity and also of forming bacterial biofilm. Additional properties enabling S. mutans to colonize the oral cavity include the ability to survive in an acidic environment and specific interaction with other microorganisms colonizing this ecosystem. This review is an attempt to establish which characteristics associated with biofilm formation—virulence determinants of S. mutans—are responsible for the development of dental caries. In order to extend the knowledge of the nature of Streptococcus infections, an attempt to face the following problems will be made: Biofilm formation as a complex process of protein–bacterium interaction. To what extent do microorganisms of the cariogenic flora exemplified by S. mutans differ in virulence determinants “expression” from microorganisms of physiological flora? How does the environment of the oral cavity and its microorganisms affect the biofilm formation of dominant species? How do selected inhibitors affect the biofilm formation of cariogenic microorganisms?

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“…Mechanical plaque removal methods such as tooth brushing and dental flossing have been advocated as reducing the biofilm formation. However, these mechanical plaque removal methods require good manual dexterity and can be difficult to implement in certain circumstances, such as in older patients [ 2 , 14 ]. Exposed dentine on the root surface is more susceptible to caries than enamel.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Cariogenic Plaque Formation on Root Surface with Polydopamine-Induced-Polyethylene Glycol Coating

Mei

Chu

2016

Materials

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Root caries prevention has been a challenge for clinicians due to its special anatomical location, which favors the accumulation of dental plaque. Researchers are looking for anti-biofouling material to inhibit bacterial growth on exposed root surfaces. This study aimed to develop polydopamine-induced-polyethylene glycol (PEG) and to study its anti-biofouling effect against a multi-species cariogenic biofilm on the root dentine surface. Hydroxyapatite disks and human dentine blocks were divided into four groups for experiments. They received polydopamine-induced-PEG, PEG, polydopamine, or water application. Contact angle, quartz crystal microbalance, and Fourier transform infrared spectroscopy were used to study the wetting property, surface affinity, and an infrared spectrum; the results indicated that PEG was induced by polydopamine onto a hydroxyapatite disk. Salivary mucin absorption on hydroxyapatite disks with polydopamine-induced-PEG was confirmed using spectrophotometry. The growth of a multi-species cariogenic biofilm on dentine blocks with polydopamine-induced-PEG was assessed and monitored by colony-forming units, confocal laser scanning microscopy, and scanning electron microscopy. The results showed that dentine with polydopamine-induced-PEG had fewer bacteria than other groups. In conclusion, a novel polydopamine-induced-PEG coating was developed. Its anti-biofouling effect inhibited salivary mucin absorption and cariogenic biofilm formation on dentine surface and thus may be used for the prevention of root dentine caries.

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Preventing root caries development under oral biofilm challenge in an artificial mouth

Cited by 14 publications

References 33 publications

Relevance of Biofilm Models in Periodontal Research: From Static to Dynamic Systems

Relevance of Biofilm Models in Periodontal Research: From Static to Dynamic Systems

The virulence of Streptococcus mutans and the ability to form biofilms

Inhibition of Cariogenic Plaque Formation on Root Surface with Polydopamine-Induced-Polyethylene Glycol Coating

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