Prevalência elevada do Alloiococcus otitidis na otite média com efusão através da PCR simultânea

Abstract

Palavras-chave: otite média, otite média secretora/microbiologia, orelha média/microbiologia, alloiococcus otitidis, PCR, criança.

Tympanostomy tube sequelae in children with otitis media with effusion: a three-year follow-up study

“…The surgical act was performed under general anesthesia. Middle ear effusion was aspirated with an Alden-Senturia collector ( Alden-Senturia collector ® , Storz Instruments, St. Louis, USA), because it had been included in a study about the presence of bacteria in middle ear effusion of children with OME 8 . Short-term ventilation tubes, made of silicone, measuring 1.2 x 2.6 mm, type Donaldson ( Medicone ® , Pomp Produtos Hospitalares, Cachoeirinha, Brazil) were placed on the anterior-inferior quadrant of the TM.…”

Bacterial Pathogens Related to Chronic Suppurative Otitis Media in Individuals with Cleft Palate: Bacteriological Culture and Polymerase Chain Reaction

“…The PCR technique has been often applied for diagnosis of otitis media with effusion and presents some advantages such as practicability, speediness, simplicity, unlimited time for analysis, high specificity, and high sensitivity; besides, the microbial samples do not need to be viable. Several studies demonstrate that PCR is a very effective method for the diagnosis of CSOM (Post et al, 1995; Matar et al, 1998; Post et al, 1998; Rayner et al, 1998; Gok et al, 2001; Park et al, 2004; Pereira et al, 2004).…”

“…It has been cultured in 5% of middle ear effusions on BHI Agar plates supplemented with rabbit blood. However, it has been detected in up to 52% when the PCR technique is used (Leskinen et al, 2002; Pereira et al, 2004). The exact role of A. otitidis in the pathogenesis of CSOM is not known in individuals with and without clefts; yet, its presence in pure cultures may suggest that it is a potential pathogen of the middle ear.…”

“…Recent studies using the PCR technique have identified genomic sequences of bacteria and viruses in negative cultures from middle ear effusions (Post et al, 1995; Matar et al, 1998; Post et al, 1998; Rayner et al, 1998; Gok et al, 2001; Pereira et al, 2004).…”

Reviewing the Pathogenic Potential of the Otitis-Associated Bacteria Alloiococcus otitidis and Turicella otitidis

“…In 1997, A. otitidis was incorporated as part of a multiplex PCR method with the three major otopathogens, NTHi, S. pneumonia, and M. catarrhalis (Hendolin et al, 1997). The use of PCR has substantially improved the detection rate of these four organisms in MEF specimens compared to microbiological culture, as demonstrated by studies where both methods were used (Hendolin et al, 1997(Hendolin et al, , 1999Leskinen et al, 2002;Pereira et al, 2004;Harimaya et al, 2006a;Kaur et al, 2010;Aydin et al, 2012;Khoramrooz et al, 2012;Sheikh et al, 2015;Sillanpää et al, 2016;Slinger et al, 2016). In children with OME or COME, A. otitidis has been reported by PCR in 18.5-60.5% of MEF specimens (13 studies), and in children with AOM or rAOM, 7-50% (5 studies).…”