Prevalência de Mycoplasma bovis em rebanhos de vacas leiteiras

Manzi, Marcela de Pinho; Joaquim, Sâmea Fernandes; Guimarães, F. F.; Bruder‐Nascimento, Ariane; Pantoja, J.C.F.; Langoni, Hélio

doi:10.1590/1678-5150-pvb-5192

Cited by 5 publications

(5 citation statements)

References 17 publications

(15 reference statements)

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“…The frequency of M. bovis infection was compatible with previous research carried out in Brazil by PRETTO et al (2001), JUNQUEIRA (2017), MANZI et al (2018) andSALINA et al (2020) who detected an occurence ranging from 1 to 3%. In Australia, low frequencies for M. bovis infection were reported by MORTON et al (2014), who stated that; although, the microorganism is present in less than 1.0% of herds, it can cause severe disease in some of them.…”

Section: Resultssupporting

confidence: 91%

“…Thus, it is difficult to compare the occurrence of the most prevalent species of these agents in the country (SALINA et al, 2020). The first isolation of M. bovis from bovine milk occurred in a mastitis outbreak in Paraná (METTIFOGO et al, 1996) and, since then, few studies have been conducted, restricting the identification of the agent in herds in Paraná, São Paulo, Minas Gerais and Goiás, with occurence in animals ranging from 1 to 3% (PRETTO et al, 2001;FRANCHESCHINI et al, 2008;JUNQUEIRA, 2017;JOAQUIM et al, 2018;MANZI et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Risk factors associated with intramammary colonization with Mollicutes in dairy cattle from Southeast Brazil

et al. 2021

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Bacteria of Mollicutes Class are associated with intramammary infection and decrease in milk production. This study investigated the occurrence of Mollicutes and elucidated their risk factors in dairy herds from Southeast Brazil. For this, milk samples from 387 lactation cows from Minas Gerais, Rio de Janeiro and São Paulo States were subjected to the polymerase chain reaction (PCR) to detect Mollicutes. Species of Mycoplasma were investigated in Mollicutes positive samples by PCR, including Mycoplasma bovis, M. alkalescens, M. bovigenitalium, M. bovirhinis, M. arginini and A. laidlawii. An epidemiological questionnaire was applied to collect data on possible risk factors, which were assessed using Pearson’s Chi-square test followed by odds ratio (P≤0.05). Mollicutes were reported in 21% (4/19) of the herds and 4% (16/387) of the animals, while 1% (5/387) were positive for M. bovis and 3% (11/387) for M. arginini. All samples were negative to the other agents. Herds with more than 150 animals [OR=3.51 (95% CI 1.11-11.08)], manual milking [OR=9.97 (95% CI 2.80-35.49)] and not-milking animals with mastitis last [OR=6.54 (95% CI 1.92-22.29)] were risk factors. The presence of these conditions may favor intramammary infection by Mollicutes in dairy herds from Southeast Brazil. This is the first report of M. bovis in Rio de Janeiro and M. arginini in the studied states.

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Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Risk factors associated with intramammary colonization with Mollicutes in dairy cattle from Southeast Brazil

et al. 2021

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“…Junqueira et al (2017) reported the occurrence of M. bovis (3%) in samples of bovine clinical mastitis. Manzi et al (2018), in a study regarding the occurrence of M. bovis in milk expansion tanks, obtained 1.4% for the same species.…”

Section: Introductionmentioning

confidence: 91%

“…The literature provides data related only to the occurrence of M. bovis in dairy herds. In milk expansion tanks, Manzi et al (2018) obtained a 1.4% prevalence of M. bovis, in 67 tank samples analyzed. Junqueira et al (2017) analyzed milk samples from clinical mastitis and reported the occurrence of 3% of M. bovis detected by conventional PCR.…”

Section: Molecular Analysismentioning

confidence: 99%

Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis

et al. 2020

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The genus Mycoplasma includes more than 200 bacterial species that cause disease in animals. It is responsible for causing mastitis in bovines and may be related to other manifestations, such as arthritis and pneumonia in calves and heifers. The present study aimed to detect Mycoplasma bovis isolated from milk samples of bovine clinical mastitis, and to compare the isolation rates in two culture media: Hayflick and SP4. An initial screening was performed in order to detect the presence of the class Mollicutes in 1166 milk samples from clinical mastitis by the conventional Polymerase Chain Reaction (PCR) technique. According to the 1166 milk samples evaluated, 8.6% (100/1166) were positive to class Mollicutes. Regarding molecular analyses, 1.1% (13/1166) of conventional PCR for positive M. bovis was obtained and 0.9% (11/1166) in real-time PCR. The results of the microbiological culture of the 100 samples previously screened demonstrated that 6% (6/100) of colony growth have been developed when using the Hayflick medium, and 11% (11/100) when using the SP4 medium (including the positive on Hayflick medium). Concerning the 11 isolates obtained in the microbiological culture, conventional PCR confirmed M. bovis in nine of them, and two cultures were negative. In the phylogenetic analysis of the isolates, all of them were grouped in M. bovis and M. agalactiae clusters. The results confirmed the importance of the presence of M. bovis in the etiology of bovine clinical mastitis and reinforced the need for further studies to elucidate other Mycoplasma species that may be involved in bovine clinical mastitis in Brazil.

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“…The positivity of Mollicutes in Brazilian herds can vary and was demonstrated in previous studies with 3.0% and 8.6% of clinical mastitis samples [16,17] and 16.4% of bulk tanks positive for this class of microorganisms [33]. In other countries, positivity is also variable: in the USA, it varies from 1 to 8% [34]; in Greece, to 5.4% [35]; in Thailand, to 1.8% [36]; and in Egypt, to 7.53% [37].…”

Section: Discussionmentioning

confidence: 87%

The Efficiency of Centrifugation in the Detection of Mollicutes in Bovine Milk

Salina,

Lopes,

Barone

et al. 2023

Ruminants

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Mastitis is one of the main diseases that affects dairy cattle. It is characterized by a multifactorial disease with multiple etiologies including microorganisms such as bacteria, viruses, fungi, yeasts, and algae. Mastitis caused by Mycoplasma spp. results in a contagious nature of infection and has been studied much in Brazil. The objectives of this research were (1) to investigate in clinical mastitis milk samples, by conventional PCR, the presence of the following mycoplasma species: Mycoplasma (M.) bovis, M. bovirhinis, M. bovigenitalium, M. californicum, and M. alkalescens and (2) compare the PCR Mollicutes detection rates using previous concentration and homogenization steps of the milk samples. Of the 993 clinical mastitis milk samples analyzed, 13.7% (136/993) and 8.0% (80/993) were positive for the Mollicutes class after concentration and homogenization, respectively, and all of them were PCR-negative for the five species investigated. Of the 70 expansion milk tanks analyzed, all of them were PCR-negative for the Mollicutes class. The importance of the previous concentration of milk in the routine of molecular analysis was evidenced when compared to the results obtained only with homogenization.

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Prevalência de Mycoplasma bovis em rebanhos de vacas leiteiras

Cited by 5 publications

References 17 publications

Risk factors associated with intramammary colonization with Mollicutes in dairy cattle from Southeast Brazil

Risk factors associated with intramammary colonization with Mollicutes in dairy cattle from Southeast Brazil

Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis

The Efficiency of Centrifugation in the Detection of Mollicutes in Bovine Milk

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