Prevalencia de cepas del grupo de Bacillus cereus productoras de biopelicula en helados comercializados en México

Adame-Gómez, Roberto; Alarcón, Natividad Castro; Vences-Velázquez, Amalia; Rodríguez-Bataz, Elvia; Santiago-Dionisio, Maria Cristina; Ramírez-Peralta, Arturo

doi:10.5281/zenodo.3520530

Cited by 1 publication

(3 citation statements)

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“…In Mexico, B. cereus is currently not included in the sanitary legislation of any product. However, the circulation of this microorganism in different food matrices has been reported [ 49 , 50 , 51 ]. Regarding dairy products, the highest frequency has been reported in QF [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

“…Regarding the toxigenic profile, most of the strains presented at least one gene for one of the diarrheagenic toxins of B. cereus ; the most common gene was the nonhemolytic toxin (Nhe); in this sense, the high frequency of genes of this toxin has been reported in strains isolated from the farm environment [ 27 , 30 ] and different dairy products [ 22 , 23 , 53 , 54 , 55 ], including those reported in the region. It is important to emphasize that the presence of the BL toxin gene has not been reported in the studies conducted in the region, including in the dairy industry [ 25 , 49 , 50 , 51 ]. However, in this study, the presence was found in strains isolated from farm air, which is consistent with what has been reported in dairy products in studies of other regions [ 22 , 23 , 53 , 54 , 55 ], and in farm environments [ 27 , 30 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that B. cereus can form biofilms, which prevents its eradication from different food environments because the spores attached to the biofilm are protected from various disinfectants [ 58 ]. Therefore, it was important to determine the production of biofilms, and it was found that most of the strains produce biofilms on the glass; this property has been reported in strains isolated from ice cream [ 51 ]. Several operons have been described in biofilm formations that have multiple functions: the eps2 operon has been related to bacterial adhesion to surfaces, cell-cell interaction, cell aggregation, and biofilms, while the eps1 operon has been related to bacterial motility [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

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Bacillus cereus in the Artisanal Cheese Production Chain in Southwestern Mexico

Cruz-Facundo,

Toribio-Jiménez,

Castro-Alarcón

et al. 2023

Microorganisms

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Background: Bacillus cereus is associated with milk, dairy product, and dairy farm contamination. The aim of this study was to characterize strains of B. cereus in the small-scale artisanal cheese production chain in southwestern Mexico. Methods: 130 samples were collected. B. cereus isolation was performed on Mannitol Egg Yolk Polymyxin (MYP) agar. Genotyping, enterotoxigenic profile, and determination of genes involved in the formation of B. cereus biofilm were performed by PCR. An antimicrobial susceptibility test was made by broth microdilution assay. The phylogenetic analysis was performed by amplification and sequencing of 16s rRNA. Results: B. cereus sensu lato was isolated and molecularly identified in 16 samples and B. cereus sensu stricto (B. cereus) was the most frequently isolated and identified species (81.25%). Of all the isolated B. cereus sensu lato strains, 93.75% presented at least one gene for some diarrheagenic toxins, 87.5% formed biofilms, and 18.75% were amylolytic. All B. cereus sensu lato strains were resistant to beta-lactams and folate inhibitors. A close phylogenetic relationship between isolates was found between the cheese isolates and the air isolates. Conclusions: Strains of B. cereus sensu lato were found in small-scale artisanal cheeses on a farm in southwestern Mexico.

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