Prevalência da imunodeficiência severa combinada em cavalos da raça Árabe em plantéis de Minas Gerais e São Paulo

Abstract

Neste estudo foram genotipados 205 cavalos da raça Árabe, criados nos estados de Minas Gerais e São Paulo, via DNA por meio de PCR, para determinação da presença do gene mutante SCID. Os resultados mostraram 98,5% de animais normais (202/205) e 1,5% de portadores (3/205). Pela análise da genealogia dos portadores identificados pode-se, ainda, confirmar a participação de um garanhão anteriormente identificado como provável disseminador da doença.

Investigation of the SCID mutant allele in Turkish Arabian horses reared in some private farms of the Eskişehir region in Turkey

“…Several previous genetic studies have investigated the five nucleotide deletion in the DNA-PKcs gene using primers that yield a PCR product of 521 bp [25], 259 bp [15], 235 bp [16], 179 bp [10], 174 bp [22], 169 bp [8,26], 163 bp [1,9,19,27], 162 bp [11], and 135 bp [13,17] in different Arabian horse populations.…”

“…To detect wild and mutant SCID alleles, the amplified PCR products were electrophoresed on 10% [10], 8% [13,15], and 6% Polyacrylamide gel [8,27], 4% agarose gel [17], or capillary electrophoresis [9,11,16,19]. In addition to electrophoresis, sequencing analysis could be performed to identify the genotypes of a population in terms of the presence/absence of SCID mutant alleles, as in these studies [1,25].…”

“…The studies that investigated SCID in Arabian horses in the literature were summarized in Table. The SCID mutant allele was not observed in Arabian horse populations in Poland [18], Romania [16], Slovenia [17], Latvia [27], Iran [15], Syria [14], Ukraine [13], Egypt [1,28], or Turkey [19]. Conversely, the SCID mutant allele was identified in Arabian horse populations in the following prevalence: 8.4% in the USA [8], 2.8% in England [9], 1.5% in Brazil [10], 6.4% (in the year 2004) and 3.4% (in the year 2009) in South Africa [12], 5.6% in Morocco [11], 1.6% in Iran [26], and 0.4% in Poland [25].…”

“…Among Arabian horse populations tested using molecular methods, the SCID mutant gene was detected in the USA [8], the UK [9], Brazil [10], Morocco [11], Egypt [1], and South Africa [12], but could not be detected in Ukraine [13], Syria [14], Iran [15], Romania [16], Slovenia [17], Poland [18], or Turkey [19]. There was only one study on SCID in Turkish Arabian horses.…”

Investigation of severe combined immunodeficiency (SCID) disease of Arabian horses raised at the state stud farms in Turkey

“…After development of reliable and useful DNAbased tests, contemporary incidence values of SCID carrier and clinically affected foals have been increasingly reported from different countries (3,11,14,21,20,22,26). For example, it is reported that 17 of 204 (8.3%) foals are found SCID affected in Australia (11).…”