Prevalence study and phylogenetic analysis of group C porcine rotavirus in the Czech Republic revealed a high level of VP6 gene heterogeneity within porcine cluster I1

Abstract

Group C rotavirus (RVC) has been described to be a causative agent of gastroenteritis in humans and animals. In the current study, the presence of porcine RVC was confirmed in 25.6 % of 293 porcine faecal samples collected from seven Czech farms. A significantly larger (p < 0.05) number of RVC-positive samples was detected in groups of finisher pigs and post-weaning piglets (4-12 weeks of age). Phylogenetic analysis of nine RVC-positive Czech strains and their comparison with available sequence data for the gene encoding RVC group antigen VP6 revealed two separate lineages within porcine cluster I1.

VP6 gene diversity in 11 Brazilian strains of porcine group C rotavirus

“…1), according to the recently proposed RVC VP6 gene classification [18]. Similar results were described by Moutelíková et al [17] and Suzuki et al [18], who showed that porcine RVC strains from within a country grouped into distinct porcine clusters. Despite using a partial fragment (230 bp) for Virus Genes Fig.…”

“…The porcine RVC strains have been demonstrated to have higher diversity upon VP6 gene analysis [17,18] compared with human [34,35] and bovine [36] strains. Additionally, previous studies have described high nucleotide similarity between strains from different host species [15,20].…”

“…Complete molecular analyses of the VP6 gene of porcine RVC have only been carried out in the United States [16,31], South Korea [15,32], the Czech Republic [17], and Japan [18]. In the present study, amplicons of 1,353 bp of the complete RVC VP6 gene were amplified by RT-PCR from all 15 faecal samples that were positive for the partial VP6 RVC fragment.…”

“…This virus has been associated with diarrhoea mainly in weaning and postweaning pigs, either alone or in mixed infection with other enteric pathogens [12][13][14]. Studies using a reverse transcription polymerase chain reaction (RT-PCR) technique demonstrated high detection rates of RVC infection in piglets from several countries, ranging from 19.5 to 46 % [12,13,[15][16][17][18]. In Brazil, a previous study had shown that RVC is an important pathogen associated with diarrhoea outbreaks in suckling piglets, with high rates of morbidity (70-80 %) and lethality (20-25 %) [19].…”

Analysis of the full genome of human group C rotaviruses reveals lineage diversification and reassortment

“…All human RVCs share high conservation within the VP7 and VP4 genes and are classified as G4P [2]. The limited number of genomic sequence data on RVCs hinders the establishment of a proper classification system for complete genomic designation of RVC strains (Mawatari et al, 2014;Moutelíkov a et al, 2014), similar to the classification system developed for RVAs (Matthijnssens et al, 2008). Implementing a classification system based on the 11 genome segments would be important to better understand the epidemiology of RVC infections (Baek et al, 2013;Marton et al, 2015;Yamamoto et al, 2011;Zhirakovskaia et al, 2016).…”