Prevalence, sequence types, antibiotic resistance and, gyrA mutations of Salmonella isolated from retail fresh chicken meat in Singapore

Zwe, Ye Htut; Tang, Vivien Chia Yen; Aung, Kyaw Thu; Gutiérrez, Ramona Alikiiteaga; Ng, Lee Ching; Yuk, Hyun‐Gyun

doi:10.1016/j.foodcont.2018.03.004

Cited by 53 publications

(47 citation statements)

References 45 publications

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“…In Singapore, the estimated occurrence of Salmonella, ranging from 2.7% to 41.3%, was reported in various types of poultry meat across the food chain. While Salmonella was rarely found in retail food (0.08%, 17/21,428), the majority of cooked or ready-to-eat food samples detected with Salmonella contained poultry meat or eggs [5,8]. The current knowledge therefore suggests poultry and eggs as relatively high-risk ingredients for Salmonella contamination.…”

Section: Introductionmentioning

confidence: 99%

Distribution of Salmonella Serovars in Humans, Foods, Farm Animals and Environment, Companion and Wildlife Animals in Singapore

Aung

Khor²,

Octavia

et al. 2020

IJERPH

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We analyzed the epidemiological distribution of Salmonella serovars in humans, foods, animals and the environment as a One-Health step towards identifying risk factors for human salmonellosis. Throughout the 2012–2016 period, Salmonella ser. Enteritidis was consistently the predominating serovar attributing to >20.0% of isolates in humans. Other most common serovars in humans include Salmonella ser. Stanley, Salmonella ser. Weltevreden, Salmonella ser. Typhimurium and Salmonella ser. 4,5,12:b:-(dT+). S. Enteritidis was also the most frequent serovar found among the isolates from chicken/chicken products (28.5%) and eggs/egg products (61.5%) during the same period. In contrast, S. Typhimurium (35.2%) and Salmonella ser. Derby (18.8%) were prevalent in pork/pork products. S. Weltevreden was more frequent in seafood (19.2%) than others (≤3.0%). Most isolates (>80.0%) from farms, companion and wildlife animals belonged to serovars other than S. Enteritidis or S. Typhimurium. Findings demonstrate the significance of a One-Health investigative approach to understand the epidemiology Salmonella for more effective and integrated surveillance systems.

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Section: Introductionmentioning

confidence: 99%

Distribution of Salmonella Serovars in Humans, Foods, Farm Animals and Environment, Companion and Wildlife Animals in Singapore

Aung

Khor²,

Octavia

et al. 2020

IJERPH

Self Cite

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“…Recently, various researches have been focused on the presence of Salmonella spp. in poultry meat (Van et al, 2007;Pointon et al, 2008;Yang et al, 2011;Zwe et al, 2018). This study has shown the high of Salmonella contamination (33.3%) in chicken meats.…”

Section: Results and Discussion General Microbiological Quality Charamentioning

confidence: 57%

“…The presence of Salmonella spp. in investigated chicken meat samples might stem from the unhygienic conditions (Çetin, 2006;Procura et al, 2017;Zwe et al, 2018). Relatively inferior hygiene practices, such as cutting chickens with knife and chopping board without proper cleaning, and exhibiting chicken carcasses in the chillers without physical separation or individual packaging could likely contribute to cross-contamination events leading to a significantly higher rate of Salmonella spp.…”

Section: Results and Discussion General Microbiological Quality Charamentioning

confidence: 99%

Determination of Microbiological Quality and Presence of Salmonella Spp. On Chicken Parts Sold at Retail Markets in Erzurum, Antibiotic Resistance of the Salmonella Spp. Isolates

Yüksel

Çeti̇n

Sert

2019

Gıda

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The present study was conducted to investigate the certain microbiological quality characteristics, and presence of Salmonella spp. by immunomagnetic separation (IMS) and conventional method, also to evaluate the antibiotic resistance of Salmonella spp. isolates obtained from 45 chicken meat samples (fifteen livers, chests, baguettes) collected from markets at the Erzurum. Totally, in the 15 samples were found positive for Salmonella spp. by IMS and conventional methods. Isolates were identified with 99.9% probability using API 20E biochemical analysis kit. The results of antibiotic test showed the susceptibility of the isolates to ciprofloxacin, chloramphenicol and gentamicin at 100% level, while these isolates were resistance to tetracycline, trimethoprim, sulfamethoxazole/trimethoprim, nalidixic acid, streptomycin, ampicillin and kanamycin, respectively. The obtained results showed that 84% (38/45) of the chicken meat samples did not show suitability with Turkish Food Codex in terms of total bacteria count and presence of Salmonella in 33% (15/45) of the samples.

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“…Saintpaul strains previously isolated from raw chicken from supermarkets and wet markets of Singapore (Zwe et al . 2018) and their relevant information is presented in Table 2. The W13‐D strain chosen to be the donor for the conjugation process was found to be a carrier of an IncI1 Sequence Type 71 plasmid containing an ESBL bla CTX‐M‐65 gene which confers resistance to third‐generation cephalosporins such as ceftiofur and ceftriaxone.…”

Section: Methodsmentioning

confidence: 99%

Prior exposure of agriculture cephalosporin ceftiofur impaired conjugation of bla _CTX‐M‐65 gene‐bearing plasmid in Salmonella Saintpaul

Zwe

Yuk

2020

J Appl Microbiol

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Aims Although a link between agricultural cephalosporin use and resistance in Salmonella has been demonstrated with the drug ceftiofur, the underlying mechanism of the correlation is unclear. This study investigated the impact of ceftiofur exposure in S. Saintpaul on ceftriaxone resistance, the gene expression and the conjugative transfer of the blaCTX‐M‐65 gene. Methods and Results Prior ceftiofur exposure caused a twofold increase in MIC from 1024 to 2048 µg ml−1 towards ceftriaxone and increased the enzymatic activity of BlaCTX‐M‐65 2·2 folds from 3·46 to 7·67 nmol nitrocefin hydrolysed min−1. A threefold upregulation in gene expression of the blaCTX‐M‐65 gene was also observed. Donors exposed to ceftiofur subsequently demonstrated a 2·5‐fold decrease in transfer efficiency. Conclusions Prior exposure of S. Saintpaul to ceftiofur led to increased phenotypic resistance towards ceftriaxone while its ability to spread the cephalosporin resistance through conjugation, conversely, was impaired. Significance and Impact of the Study Findings from this study shed light on one possible mechanism in which agricultural cephalosporin exposure in Salmonella may subsequently impact clinical treatment. The finding that cephalosporin exposure in donors may hinder the subsequent spread of resistance instead of aiding it up was counter‐intuitive.

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Prevalence, sequence types, antibiotic resistance and, gyrA mutations of Salmonella isolated from retail fresh chicken meat in Singapore

Cited by 53 publications

References 45 publications

Distribution of Salmonella Serovars in Humans, Foods, Farm Animals and Environment, Companion and Wildlife Animals in Singapore

Distribution of Salmonella Serovars in Humans, Foods, Farm Animals and Environment, Companion and Wildlife Animals in Singapore

Determination of Microbiological Quality and Presence of Salmonella Spp. On Chicken Parts Sold at Retail Markets in Erzurum, Antibiotic Resistance of the Salmonella Spp. Isolates

Prior exposure of agriculture cephalosporin ceftiofur impaired conjugation of bla _CTX‐M‐65 gene‐bearing plasmid in Salmonella Saintpaul

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Prevalence, sequence types, antibiotic resistance and, gyrA mutations of Salmonella isolated from retail fresh chicken meat in Singapore

Cited by 53 publications

References 45 publications

Distribution of Salmonella Serovars in Humans, Foods, Farm Animals and Environment, Companion and Wildlife Animals in Singapore

Distribution of Salmonella Serovars in Humans, Foods, Farm Animals and Environment, Companion and Wildlife Animals in Singapore

Determination of Microbiological Quality and Presence of Salmonella Spp. On Chicken Parts Sold at Retail Markets in Erzurum, Antibiotic Resistance of the Salmonella Spp. Isolates

Prior exposure of agriculture cephalosporin ceftiofur impaired conjugation of bla CTX‐M‐65 gene‐bearing plasmid in Salmonella Saintpaul

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Prior exposure of agriculture cephalosporin ceftiofur impaired conjugation of bla _CTX‐M‐65 gene‐bearing plasmid in Salmonella Saintpaul