Prevalence, quantification and isolation of pathogenic shiga toxin Escherichia coli O157:H7 along the production and supply chain of pork around Hubei Province of China

Khan, Sher Bahadar; Zou, Geng; Xiao, Ran; Cheng, Yu-Ting; Rehman, Zia Ur; Ali, Sher; Memon, Ashraf; Fahad, Shah; Ahmad, Irshad; Zhou, Rui

doi:10.1016/j.micpath.2017.12.019

Cited by 10 publications

(8 citation statements)

References 28 publications

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“…2). Although there was no STEC O157 being isolated from pigs in Central China in the present study, a recent study has revealed that the overall prevalence of E. coli O157:H7 in pig farms around Hubei, a province located in Central China, is approximately 12.8% (16/125) [13]. These findings suggest that the prevalence of E. coli O157 in this region is still a problem.…”

Section: Discussioncontrasting

confidence: 58%

“…While limited data on STEC in humans in China are available, both STEC O157 and non-O157 STEC including some predominant serogroups associated with human disease, such as O26, O45, O103, O111, and O121, have been detected and isolated from domestic and wild animals as well as raw meats in different regions [11–14]. A recent study has revealed that the overall prevalence of STEC O157:H7 was 41.3% along the production and supply chain of pork around Hubei Province in Central China, and the prevalence found in slaughter houses, wet- and super-markets were 86.25% (69/80), 53.3% (32/60), and 28.3% (17/60), respectively [13]. These data suggest a big threat to the food safety and even human health in this region.…”

Section: Introductionmentioning

confidence: 99%

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O-serogroups, virulence genes, antimicrobial susceptibility, and MLST genotypes of Shiga toxin-producing Escherichia coli from swine and cattle in Central China

Peng

Liang

et al. 2019

BMC Vet Res

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BackgroundShiga toxin-producing Escherichia coli (STEC) is a leading cause of worldwide food-borne and waterborne infections. Despite an increase in the number of STEC outbreaks, there is a lack of data on prevalence of STEC at the farm level, distribution of serogroups, and virulence factors.ResultsIn the present study, a total of 91 (6.16%) STEC strains were isolated from 1477 samples including pig intestines, pig feces, cattle feces, milk, and water from dairy farms. The isolation rates of STEC strains from pig intestines, pig feces, and cattle feces were 7.41% (32/432), 4.38% (21/480), and 9.57% (38/397), respectively. No STEC was isolated from the fresh milk and water samples. By O-serotyping methods, a total of 30 types of O-antigens were determined, and the main types were O100, O97, O91, O149, O26, O92, O102, O157, and O34. Detection of selected virulence genes (stx1, stx2, eae, ehxA, saa) revealed that over 94.51% (86/91) of the isolates carried more than two types of virulence associated genes, and approximately 71.43% (65/91) of the isolates carried both stx1 and stx2, simultaneously. Antimicrobial susceptibility tests showed that most of the STEC isolates were susceptible to ofloxacin and norfloxacin, but showed resistance to tetracycline, kanamycin, trimethoprim-sulfamethoxazole, streptomycin, amoxicillin, and ampicillin. MLST determined 13 categories of sequence types (STs), and ST297 (31.87%; 29/91) was the most dominant clone. This clone displayed a close relationship to virulent strains STEC ST678 (O104: H4). The prevalence of ST297 clones should receive more attentions.ConclusionsOur preliminary data revealed that a heterogeneous group of STEC is present, but the non-O157 serogroups and some ST clones such as ST297 should receive more attentions.

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Section: Discussioncontrasting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

O-serogroups, virulence genes, antimicrobial susceptibility, and MLST genotypes of Shiga toxin-producing Escherichia coli from swine and cattle in Central China

Peng

Liang

et al. 2019

BMC Vet Res

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“…Louise et al () reported that Stx1 and Stx2 proteins cause different degrees and types of tissue damage, with Stx2 being more toxic than Stx1 for human renal endothelial cells. Khan et al () have recently demonstrated a higher potential for cell death in mice when E. coli O157:H7 harbours both genes, stx1 and stx2 , as observed in the isolate obtained in this study. Another important determinant of virulence in STEC is the gene encoding the outer membrane adhesin, intimin ( eae ), which promotes the binding of the pathogen to the intestinal mucosa of the host (Ito et al ; Sallam et al ).…”

Section: Discussionsupporting

confidence: 76%

First report ofEscherichia coliO157:H7 in ready‐to‐eat sushi

et al. 2019

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Aims: The aim of this study was to evaluate the microbiological quality of commercially prepared ready-to-eat (RTE) sushi by enumerating aerobic mesophilic bacteria (AMB) and thermotolerant coliforms (TC) and detecting Escherichia coli and Salmonella ssp. An isolate was identified as E. coli O157:H7 which was evaluated for its virulence and antimicrobial resistance profiling as well as its ability to form biofilms on stainless steel. Methods and Results: There were four sampling events in seven establishments, totalling 28 pools of sushi samples. Mean AMB counts ranged between 5Á2 and 7Á7 log CFU per gram. The enumeration of TC varied between 2Á1 and 2Á7 log MPN per gram. Salmonella ssp. were not detected, and one sample was positive for E. coli and was identified as E. coli O157:H7. To the best of our knowledge, this is the first report of E. coli O157:H7 in sushi samples in the world literature. This isolate presented virulence genes stx1, stx2, eae and hlyA. It was also susceptible to 14 antimicrobials tested and had the ability to form biofilms on stainless steel. Conclusions: There is a need to improve the good hygiene practices adopted in establishments selling sushi in the city of Pelotas, Brazil. In addition, the isolated E. coli O157:H7 carries a range of important virulence genes being a potential risk to consumer health, as sushi is a RTE food. This isolate also presents biofilm formation ability, therefore, may trigger a constant source of contamination in the production line of this food. Significance and Impact of the Study: The increase in the consumption of sushi worldwide attracts attention regarding the microbiological point of view, since it is a ready-to-eat food. To our knowledge, this was the first time that E. coli O157:H7 was identified in sushi samples.

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“…Various routes, including physical, chemical and biological procedures, have been developed to synthesize silver nanoparticles. However, an increased demand in eco-friendly processes and using biocompatible reagents make a great of interest the biological way of synthesis of nanoparticle 3 . During the green synthesis, biological compounds from bacteria, microalga and yeasts are used, instead of chemical formulas, which makes the reaction more environmentally friendly.…”

Section: Introductionmentioning

confidence: 99%

Noncytotoxic silver nanoparticles as a new antimicrobial strategy

Skóra

Krajewska

Nowak

et al. 2021

Sci Rep

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Drug-resistance of bacteria is an ongoing problem in hospital treatment. The main mechanism of bacterial virulency in human infections is based on their adhesion ability and biofilm formation. Many approaches have been invented to overcome this problem, i.e. treatment with antibacterial biomolecules, which have some limitations e.g. enzymatic degradation and short shelf stability. Silver nanoparticles (AgNPs) may be alternative to these strategies due to their unique and high antibacterial properties. Herein, we report on yeast Saccharomyces cerevisiae extracellular-based synthesis of AgNPs. Transmission electron microscopy (TEM) revealed the morphology and structure of the metallic nanoparticles, which showed a uniform distribution and good colloid stability, measured by hydrodynamic light scattering (DLS). The energy dispersive X-ray spectroscopy (EDS) of NPs confirms the presence of silver and showed that sulfur-rich compounds act as a capping agent being adsorbed on the surface of AgNPs. Antimicrobial tests showed that AgNPs inhibit the bacteria growth, while have no impact on fungi growth. Moreover, tested NPs was characterized by high inhibitory potential of bacteria biofilm formation but also eradication of established biofilms. The cytotoxic effect of the NPs on four mammalian normal and cancer cell lines was tested through the metabolic activity, cell viability and wound-healing assays. Last, but not least, ability to deep penetration of the silver colloid to the root canal was imaged by scanning electron microscopy (SEM) to show its potential as the material for root-end filling.

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Prevalence, quantification and isolation of pathogenic shiga toxin Escherichia coli O157:H7 along the production and supply chain of pork around Hubei Province of China

Cited by 10 publications

References 28 publications

O-serogroups, virulence genes, antimicrobial susceptibility, and MLST genotypes of Shiga toxin-producing Escherichia coli from swine and cattle in Central China

O-serogroups, virulence genes, antimicrobial susceptibility, and MLST genotypes of Shiga toxin-producing Escherichia coli from swine and cattle in Central China

First report ofEscherichia coliO157:H7 in ready‐to‐eat sushi

Noncytotoxic silver nanoparticles as a new antimicrobial strategy

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