Prevalence of β‐lactamase‐producing strains among 149 anaerobic gram‐negative rods isolated from periodontal pockets

Fosse, T.; Madinier, I.; Hitzig, C; Charbit, Yves

doi:10.1034/j.1399-302x.1999.140604.x

Cited by 54 publications

(68 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Resistance to ␤-lactams is due to the presence of␤-lactamase (FN1375), which is similar to that of Clostridium spp. (10,32). There is a second ␤-lactamase similar to the archaeal lactam utilization protein, LamB, which is involved in utilization of pyrrolidinone.…”

Section: Resultsmentioning

confidence: 99%

Genome Sequence and Analysis of the Oral Bacterium Fusobacterium nucleatum Strain ATCC 25586

et al. 2002

View full text Add to dashboard Cite

We present a complete DNA sequence and metabolic analysis of the dominant oral bacterium Fusobacterium nucleatum. Although not considered a major dental pathogen on its own, this anaerobe facilitates the aggregation and establishment of several other species including the dental pathogens Porphyromonas gingivalis and Bacteroides forsythus. The F. nucleatum strain ATCC 25586 genome was assembled from shotgun sequences and analyzed using the ERGO bioinformatics suite (http://www.integratedgenomics.com). The genome contains 2.17 Mb encoding 2,067 open reading frames, organized on a single circular chromosome with 27% GC content. Despite its taxonomic position among the gram-negative bacteria, several features of its core metabolism are similar to that of gram-positive Clostridium spp., Enterococcus spp., and Lactococcus spp. The genome analysis has revealed several key aspects of the pathways of organic acid, amino acid, carbohydrate, and lipid metabolism. Nine very-high-molecular-weight outer membrane proteins are predicted from the sequence, none of which has been reported in the literature. More than 137 transporters for the uptake of a variety of substrates such as peptides, sugars, metal ions, and cofactors have been identified. Biosynthetic pathways exist for only three amino acids: glutamate, aspartate, and asparagine. The remaining amino acids are imported as such or as di-or oligopeptides that are subsequently degraded in the cytoplasm. A principal source of energy appears to be the fermentation of glutamate to butyrate. Additionally, desulfuration of cysteine and methionine yields ammonia, H 2 S, methyl mercaptan, and butyrate, which are capable of arresting fibroblast growth, thus preventing wound healing and aiding penetration of the gingival epithelium. The metabolic capabilities of F. nucleatum revealed by its genome are therefore consistent with its specialized niche in the mouth.

show abstract

Section: Resultsmentioning

confidence: 99%

Genome Sequence and Analysis of the Oral Bacterium Fusobacterium nucleatum Strain ATCC 25586

et al. 2002

View full text Add to dashboard Cite

show abstract

“…Kuriyama et al (221) revealed that beta-lactamase was detected in 36% of the dark-pigmented Prevotella and 32% of the nonpigmented Prevotella species isolated from pus samples of oral abscesses. Other enzyme-producing oral anaerobic species include strains of the Gram-negative bacteria F. nucleatum, E. corrodens, and T. forsythia and the Gram-positive bacteria P. acnes, Actinomyces species, and Peptostreptococcus species (98,(217)(218)(219)(221)(222)(223). Bacteria that produce beta-lactamases not only may protect themselves from penicillins but also can protect other penicillinsusceptible bacteria present in a mixed community by releasing free beta-lactamase into the environment (224).…”

Section: Treatment Of the Acute Apical Abscessmentioning

confidence: 99%

“…A systematic review revealed that the overall results of laboratory studies indicate that no single antibiotic is effective in vitro against all species found in dental abscesses (209). Prevotella species have been regarded as prominent sources of resistance to beta-lactam agents in the oral cavity due to production of beta-lactamase (98,(217)(218)(219)(220). Kuriyama et al (221) revealed that beta-lactamase was detected in 36% of the dark-pigmented Prevotella and 32% of the nonpigmented Prevotella species isolated from pus samples of oral abscesses.…”

Section: Treatment Of the Acute Apical Abscessmentioning

confidence: 99%

Microbiology and Treatment of Acute Apical Abscesses

2013

View full text Add to dashboard Cite

SUMMARY Acute apical abscess is the most common form of dental abscess and is caused by infection of the root canal of the tooth. It is usually localized intraorally, but in some cases the apical abscess may spread and result in severe complications or even mortality. The reasons why dental root canal infections can become symptomatic and evolve to severe spreading and sometimes life-threatening abscesses remain elusive. Studies using culture and advanced molecular microbiology methods for microbial identification in apical abscesses have demonstrated a multispecies community conspicuously dominated by anaerobic bacteria. Species/phylotypes commonly found in these infections belong to the genera Fusobacterium , Parvimonas , Prevotella , Porphyromonas , Dialister , Streptococcus , and Treponema . Advances in DNA sequencing technologies and computational biology have substantially enhanced the knowledge of the microbiota associated with acute apical abscesses and shed some light on the etiopathogeny of this disease. Species richness and abundance and the resulting network of interactions among community members may affect the collective pathogenicity and contribute to the development of acute infections. Disease modifiers, including transient or permanent host-related factors, may also influence the development and severity of acute abscesses. This review focuses on the current evidence about the etiology and treatment of acute apical abscesses and how the process is influenced by host-related factors and proposes future directions in research, diagnosis, and therapeutic approaches to deal with this disease.

show abstract

“…Aproximadamente entre el 30 y el 85% de las cepas son productoras de betalactamasas, lo que conlleva resistencia a penicilina y a amoxicilina, pero no a amoxicilina/ácido clavulánico ni a otros betalactámi-cos estables a la acción de estas betalactamasas (39,47,49). La prevalencia de resistencia a tetraciclinas y macrólidos se sitúa en torno al 30-50% y 80-95% respectivamente (47,50,51), siendo azitromicina en general el macrólido más activo frente a bacilos gramnegativos anaerobios.…”

Section: Prevotella Spp Y Porphyromonas Sppunclassified

Bases farmacomicrobiológicas del tratamiento antibiótico de las enfermedades periodontales y periimplatarias

Linares

Martín-Herrero

2003

Avances en Periodoncia

View full text Add to dashboard Cite

RESUMENLiñares J. Martín-Herrero JE. Bases farmacomicrobiológicas del tratamiento antibiótico de las enfermedades periodontales y periimplantarias.Av Periodo n Implanto !. 2003; 15.3: 139-147. La enfermedad periodontal debe considerarse un proceso infeccioso bacteriano crónico. En su etiología, no hay una única especie bacteriana implicada, sino que podríamos considerarla como una infección polimicrobiana en la que estarían implicados diversos microorganismos. Las bacterias que se han asociado más directamente con la enfermedad periodontal son Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia,Bacteroides forsythus y Treponema denticola. Los parámetros farmacodinámicos de los antibióticos son muy útiles a la hora de seleccionar pautas posológicas. El aumento de resistencias producido en muchos periodontopatógenos en los últimos años ha relegado a algunos antibiótico s a un segundo plano. Entre la gran variedad de antibiótico s utilizados, se han obtenido buenas respuestas terapéuticas con amoxicilina/ácido clavulánico, metronidazol, clindamicina, doxiciclina y las combinaciones de metronidazol más amoxicilina y metronidazol más amoxicili-nal ácido clavulánico. PALABRAS CLAVEEnfermedad periodontal, antibióticos, resistencia, farmacodinamia. INTRODUCCIÓNLa cavidad oral forma un complejo ecosistema compuesto por más de 500 especies bacterianas, en el que podemos encontrar simultáneamente bacterias residentes, algunas de las cuales son exclusivas de esta localización, y bacterias ocasionales (1). Globalmente, los géneros Streptococcus, Peptostrep-tococcus, Veillonella, Lactobacillus, Corynebacte-rium yActinomyces representan más del 80% de toda la flora cultivable (2). Los bacilos gramnegativos facultativos son raros en adultos sanos, viéndose casi exclusivamente en pacientes con enfermedades severas, hospitalizados y ancianos (3).La proporción de una determinada especie bacteriana varía en función de la región anatómica que consideremos. Así por ejemplo en la superficie dental las especies predominantes son Porphyromonas spp, Prevotella spp y espiroquetas anaerobias por el surco gingival (2). Diversos factores se han implicado en este tropismo bacteriano, como son la adherencia selectiva de algunas bacterias por determinados tipos de células, tensión de oxígeno, potencial de oxidación-reducción, pH, etc. Aparte de estas consideraciones anatómicas, hay otra serie de factores que pueden influir en el ecosistema bucal, tales como la edad, dieta, higiene oral, presencia de

show abstract

Prevalence of β‐lactamase‐producing strains among 149 anaerobic gram‐negative rods isolated from periodontal pockets

Cited by 54 publications

References 51 publications

Genome Sequence and Analysis of the Oral Bacterium Fusobacterium nucleatum Strain ATCC 25586

Genome Sequence and Analysis of the Oral Bacterium Fusobacterium nucleatum Strain ATCC 25586

Microbiology and Treatment of Acute Apical Abscesses

Bases farmacomicrobiológicas del tratamiento antibiótico de las enfermedades periodontales y periimplatarias

Contact Info

Product

Resources

About