Prevalence of virulence-associated genes and antimicrobial resistance of Campylobacter jejuni from ducks in Gyeongnam Province, Korea

Abstract

Total 99 strains of Campylobacter spp. were isolated from 117 cases of duck's fecal samples. Among 99 strains of Campylobacter spp. isolates, 93 strains (93.9%) were C. jejuni and 6 strains (6.1%) were C. coli. Prevalence of virulence and GBS associated genes of 72 C. jejuni isolates was determined by m-PCR. Among the 10 kinds of virulence associated genes, cadF, dnaJ, flaA and ceuE genes were detected in all of C. jejuni isolates from ducks, racR, pldA, iamA, ciaB, virB11 and docC genes were 87.5%, 84.7%, 77.8%, 48.6%, 13.9% and 11.1%, respectively. Antimicrobial susceptibility test was performed on 72 C. jejuni isolates. The rate of resistance were 62.5% for oxytetracycline, 55.6% for kanamycin, 54.2% for enrofloxacin, 50% for ciprofloxacin, 37.5% for tetracycline and nalidixic acid, 18.1% for ampicillin, 15.3% for streptomycin, and 6.9% for ofloxacin. All isolates were susceptible to erythromycin. The adherence (intracellular and extracellular bacteria) abilities of the 20 isolates to INT-407 cells were between 4.21±1.27×10 4 CFU/well and 1.053±0.451×10 6 CFU/well from the isolates of cj-55 and cj-52, respectively, and that can be expressed as 0.1033% to 5.2655% to the infecting inoculum. The invasion (intracellular bacteria) abilities of the 20 isolates to INT-407 were between 1.00±1.73×10 3 CFU/well and 8.47±5.16×10 4 CFU/well from the isolates of cj-13 and cj-47, respectively, and that can be expressed as 0.0050% to 0.4235% to the infecting inoculums. The average CFU/well of 20 campylobacters isolated from ducks for adherence to and invasion were 2.646±2.886×10 5 and 3.03±2.7×10 4 respectively, and that was 1.3230±1.2139% and 0.1516±0.1343% of the starting viable inoculum. There was considerable correlation (R 2 =0.627) between the adherence and invasion ability of C. jejuni isolates for INT-407 cell.

Systematic Review and Meta-Analysis of Campylobacter Species Contamination in Poultry, Meat, and Processing Environments in South Korea

“…Meat products such as beef and pork are potential sources of Campylobacter contamination [63]. Taremi et al (2006) found the highest prevalence of Campylobacter in [32,33,36,37,40,41,45,51,58].…”

“…Figure 4. Forest plot of prevalence of Campylobacter considering environmental factors and processing environments in South Korea: (A) feces, (B) equipment, (C) wash water[32,33,36,37,40,41,45,51,58].…”