Prevalence of vacA, cagA and babA2 genes in Cuban Helicobacter pylori isolates

Torres, Leonel; Melián, Karelia; Moreno, Arlenis; Alonso, Jordis; Sabatier, Carlos Alfonso; Hernández, Mónica López; Bermúdez, Ludisleydis; Rodrı́guez, Boris L.

doi:10.3748/wjg.15.204

Cited by 78 publications

(92 citation statements)

References 40 publications

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“…Our data, however, supports the relationship between the genes cagA, cagE, vacA and babA2 that was described in previous reports [34][35][36][37] .…”

Section: Discussioncontrasting

confidence: 42%

cagE as a biomarker of the pathogenicity of Helicobacter pylori

Ramis

Vianna

Silva

et al. 2013

Rev. Soc. Bras. Med. Trop.

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Introduction:Helicobacter pylori infection is associated with gastro-duodenal diseases. Genes related to pathogenicity have been described for H. pylori and some of them appear to be associated with more severe clinical outcomes of the infection. The present study investigates the role of cagE as a pathogenicity biomarker of H. pylori compare it to cagA, vacA, iceA and babA2 genes and correlate with endoscopic diagnoses. Methods: Were collected biopsy samples of 144 dyspeptic patients at the Hospital of the Federal University of Rio Grande, Rio Grande do Sul, Brazil. After collection, the samples were sent for histological examination, DNA extraction and detection of all putative pathogenicity genes by PCR. Results: Of the 144 patients undergoing endoscopy, 57 (39.6%) presented H. pylori by histological examination and PCR by detection of the ureA gene. Based on the endoscopic diagnoses, 45.6% (26/57) of the patients had erosive gastritis, while 54.4% (31/57) had enanthematous gastritis. The genes cagA, cagE, vacAs1/m1, vacAs1/m2 and iceA1 were related to erosive gastritis, while the genes vacAs2/m2, iceA2 and babA2 were associated to enanthematous gastritis. We found a statistically significant association between the presence of cagE and the endoscopic diagnosis. However, we detect no statistically significant association between the endoscopic diagnosis and the presence of cagA, vacA, iceA and babA2, although a biological association has been suggested. Conclusions: Thus, cagE could be a risk biomarker for gastric lesions and may contribute to a better evaluation of the H. pylori pathogenic potential and to the prognosis of infection evolution in the gastric mucosa.

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“…Our data, however, supports the relationship between the genes cagA, cagE, vacA and babA2 that was described in previous reports [34][35][36][37] .…”

Section: Discussioncontrasting

confidence: 42%

cagE as a biomarker of the pathogenicity of Helicobacter pylori

Ramis

Vianna

Silva

et al. 2013

Rev. Soc. Bras. Med. Trop.

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“…The CagA seropositivity (60.6%) obtained with Helicoblot 2.1 test in this study was similar to the positivity observed in CagA genotyping of Western strains (24) and slightly lower than the cagA positive strains detected in Cuban H. pylori isolates (25). The Western blot assay with the rCagA developed here demonstrated adequate performance in comparison to HB 2.1 that contains the entire CagA antigen.…”

Section: Discussionsupporting

confidence: 81%

Clonaje y expresión de un fragmento recombinante del gen cagA de Helicobacter pylori y su evaluación preliminar en el serodiagnóstico

et al. 2013

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Introduction:Helicobacter pylori strains expressing cytotoxic CagA protein are more commonly associated with peptic ulceration, atrophic gastritis and gastric adenocarcinoma than those lacking CagA. Determination of anti-CagA antibodies, therefore, acquires a relevant clinical significance in the serological detection of H. pylori infection and disease risk prediction. However, the CagA-serology has been questioned due to the differences found in their performance evaluations in different populations. Objective: To obtain a recombinant CagA fragment useful for serodiagnosis of H. pylori infection Methods: A fragment of the cagA gene was cloned into a prokaryotic T7 RNA polymerase expression vector. A recombinant C-terminal His 6 -tagged CagA was expressed, subsequently solubilized with urea and purified by immobilized metal affinity chromatography. The performance of the recombinant protein was evaluated using 180 human serum samples with an in-house Western blot assay compared to the Helicoblot 2.1 reference test. Results: The expressed His 6 -tagged CagA showed an immunoreactive 80kDa band as was revealed by SDS-PAGE and Western blot analysis using two different specific anti-CagA polyclonal antibodies. The recombinant protein was successfully purified obtaining a 93% of purity. The performance analysis of the purified recombinant antigen showed good immunoreactivity and exhibited values of sensitivity, specificity and accuracy of 88.1%, 100% and 92.7%, respectively. Conclusion: The CagA fragment of the study may constitute a useful tool for serological diagnosis of CagA-positive H. pylori infection. Clonación y expresión de un fragmento recombinante del gen cagA de Helicobacter pylori y su evaluación preliminar en el serodiagnóstico Introducción. Las cepas de Helicobacter pylori que expresan la citotoxina CagA, se asocian más frecuentemente con úlcera péptica, gastritis atrófica y adenocarcinoma gástrico que las que carecen de esta citotoxina. Por lo anterior, el determinar la presencia de anticuerpos anti-CagA adquiere gran importancia clínica en la detección serológica de la infección por H. pylori y la predicción del riesgo de enfermedades. Sin embargo, los métodos serológicos que emplean CagA han sido cuestionados debido a las diferencias encontradas en las evaluaciones de su desempeño en diversas poblaciones. Objetivo. Obtener un fragmento recombinante de la proteína CagA para el serodiagnóstico de la infección por H. pylori. Materiales y métodos. Un fragmento del gen cagA fue clonado en un vector de expresión procariota que contenía el promotor de la T7 ARN polimerasa. El fragmento de la proteína CagA con seis histidinas en la región C-terminal, se expresó, se solubilizó con urea y se purificó por cromatografía de afinidad con iones metálicos inmovilizados. El desempeño de la proteína recombinante se evaluó empleando un método in house de Western Blot y 180 sueros humanos. Los resultados se compararon con la prueba de referencia Helicoblot 2.1. 547 Biomédica 2013;33:546-53 Recombinant CagA fragment fo...

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“…A total of 95 cagA positive H. pylori strains were isolated from antrum biopsies in a previous study involving 130 consecutive patients (77 male and 53 female) with a mean age of 49.1 (range 18 to 88) who underwent routine endoscopy due to dyspeptic complaints at CIMEQ Hospital, Havana, Cuba (22). Patients were classified in three groups (40 with duodenal ulcer, 19 with gastric ulcer, and 36 with non-ulcer dyspepsia) according to endoscopic and histological observations.…”

Section: Patients and H Pylori Isolatesmentioning

confidence: 99%

“…Genomic DNAs of the strains were extracted by CTAB method with phenol/chloroform and isopropanol precipitation as previously described (22). Purified DNAs were stored at -20ºC until use.…”

Section: Dna Extraction and Pcr Amplificationmentioning

confidence: 99%