cagE as a biomarker of the pathogenicity of Helicobacter pylori

Abstract

Introduction:Helicobacter pylori infection is associated with gastro-duodenal diseases. Genes related to pathogenicity have been described for H. pylori and some of them appear to be associated with more severe clinical outcomes of the infection. The present study investigates the role of cagE as a pathogenicity biomarker of H. pylori compare it to cagA, vacA, iceA and babA2 genes and correlate with endoscopic diagnoses. Methods: Were collected biopsy samples of 144 dyspeptic patients at the Hospital of the Federal University of Rio Grande, Rio Grande do Sul, Brazil. After collection, the samples were sent for histological examination, DNA extraction and detection of all putative pathogenicity genes by PCR. Results: Of the 144 patients undergoing endoscopy, 57 (39.6%) presented H. pylori by histological examination and PCR by detection of the ureA gene. Based on the endoscopic diagnoses, 45.6% (26/57) of the patients had erosive gastritis, while 54.4% (31/57) had enanthematous gastritis. The genes cagA, cagE, vacAs1/m1, vacAs1/m2 and iceA1 were related to erosive gastritis, while the genes vacAs2/m2, iceA2 and babA2 were associated to enanthematous gastritis. We found a statistically significant association between the presence of cagE and the endoscopic diagnosis. However, we detect no statistically significant association between the endoscopic diagnosis and the presence of cagA, vacA, iceA and babA2, although a biological association has been suggested. Conclusions: Thus, cagE could be a risk biomarker for gastric lesions and may contribute to a better evaluation of the H. pylori pathogenic potential and to the prognosis of infection evolution in the gastric mucosa.

Helicobacter pylori cagE is not associated with clinical outcomes in the Kurdistan region of Iraq

“…We have read with interest the article entitled "cagE as a biomarker of the pathogenicity of Helicobacter pylori" by Ramis et al 1 . In this study, with a sample size of 57, the authors suggested that cagE is an important prognostic indicator for developing lesions during Helicobacter pylori infection.…”

The cagE gene sequence as a diagnostic marker to identify JP2 and non‐JP2 highly leukotoxic Aggregatibacter actinomycetemcomitans serotype b strains

“…H. pylori CagE has a key role in Cag‐T4SS pilus biogenesis and is essential in H. pylori ‐induced gastritis and peptic ulceration . The cagE gene was recently suggested to represent a risk biomarker for the pathogenicity of H. pylori …”

Association of cag E Gene and LL-37 Serum Level with Helicobacter pylori-induced Peptic Ulceration in Egyptian Patients

“…Factors related to the host, the diversity of bacterial pathogenicity and the environment seem to be related to the broad clinical spectrum related to infection by H. pylori [31]. Cag E gene is one of virulence factor in the H. pylori organism affecting its pathogenesis potential effect in the gastric mucosa [32]. On the other hand, H. pylori upregulates LL-37/hCAP18 production by the gastric epithelium, suggesting that cathelicidin or its derivative LL-37 contributes to combat chronic infection with this gastric pathogen [22,33].…”