Prevalence of the phenicol resistance gene fexA in Campylobacter isolated from the poultry supply chain

Tang, Biao; Zheng, Xue Bin; Lin, Jiahui; Wu, Jing; Lin, Rumeng; Jiang, Han; Ji, Xiaofeng; Yang, Hua; Shen, Zhangqi; Xia, Fei

doi:10.1016/j.ijfoodmicro.2022.109912

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…Notably, E. faecium is on the global list of priority pathogens called ESKAPE (Tacconelli et al, 2018). Antimicrobial resistance (AMR) is recognized as a global public health crisis that urgently needs to be addressed (Li et al, 2017;Guan et al, 2022;Lin et al, 2022;Ma et al, 2022;Tang et al, 2022c). Enterococcus has inherent resistance to cephalosporins, anti-staphylococcal penicillin, amtrannan, aminoglycosides, lincoamides, and streptin (Golob et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Transmission of linezolid-resistant Enterococcus isolates carrying optrA and poxtA genes in slaughterhouses

Long

Wang

et al. 2023

Front. Sustain. Food Syst.

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IntroductionThe presence of linezolid-resistant enterococci found in animal-derived food has attracted attention for possible transmission to human-derived enterococci through the food chain. Linezolid-resistant enterococci in farms have been widely reported, but enterococci carrying antimicrobial resistance (AMR) genes poxtA, optrA, or cfr(D) in slaughterhouse environments have not been well addressed.MethodsEnterococcus was isolated from the samples collected from two slaughterhouses in Hangzhou, and the Enterococcus carrying linezolidin-resistant genes was identified by PCR. The minimum inhibitory concentration (MIC) of the Enterococcus carrying linezolidin-resistant genes was determined by microbroth dilution method. Finally, the whole genome of strains carrying two or more linezoline resistance genes was sequenced using the Oxford Nanopore TechnologyResultsHere, 291 enterococci strains were isolated from 309 samples (94.17%). A total of 4 poxtA-positive enterococci and 42 optrA-positive enterococci were identified based on PCR. The antimicrobial susceptibility test showed that the highest rate of florfenicol resistance was 97.82% and the rate of multidrug resistance (MDR) was 95.65%. Two strains carried multiple linezolid resistance genes, among which Enterococcus casseliflavus CQFYY22-063 cocarrying optrA, poxtA, and cfr(D) was isolated from the duck cecum, and Enterococcus faecium CQFYH22-006 cocarrying optrA and poxtA was isolated from slaughterhouse sewage for the first time. Furthermore, Oxford Nanopore Technology revealed that the optrA gene of strain CQFYY22-063 was located on the Inc18-type plasmid pFYY063-optrA-70K, and the poxtA and cfr(D) genes were located on the Inc18-type plasmid pFYY063- poxtA-12K. Meanwhile, the poxtA gene of strain CQFYH22-006 was located on the Rep3-type plasmid pFYH006-poxtA-25K, and the optrA gene was located on the chromosome.DiscussionTogether, linezolid resistance in slaughterhouses deserves extensive attention, indicating the need to strengthen the monitoring of different links in the food production chain within the One Health concept.

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Section: Introductionmentioning

confidence: 99%

Transmission of linezolid-resistant Enterococcus isolates carrying optrA and poxtA genes in slaughterhouses

Long

Wang

et al. 2023

Front. Sustain. Food Syst.

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“…Most of the air samples were positive with respect to the presence of resistance genes for five antibiotics: phenicol, apramycin, erythromycin, vancomycin, and sulfamethoxazole. The spread of phenicol antibiotic resistance genes was previously reported between agriculturally and human-impacted environments and ecosystems [ 44 , 45 ]. Apramycin resistance in bacteria collected from animal farms was reported in previous studies [ 46 , 47 ].…”

Section: Resultsmentioning

confidence: 99%

Exposure Levels of Airborne Fungi, Bacteria, and Antibiotic Resistance Genes in Cotton Farms during Cotton Harvesting and Evaluations of N95 Respirators against These Bioaerosols

et al. 2023

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The USA is the third-leading cotton-producing country worldwide and cotton farming is common in the state of Georgia. Cotton harvest can be a significant contributor to airborne microbial exposures to farmers and nearby rural communities. The use of respirators or masks is one of the viable options for reducing organic dust and bioaerosol exposures among farmers. Unfortunately, the OSHA Respiratory Protection Standard (29 CFR Part 1910.134) does not apply to agricultural workplaces and the filtration efficiency of N95 respirators was never field-tested against airborne microorganisms and antibiotic resistance genes (ARGs) during cotton harvesting. This study addressed these two information gaps. Airborne culturable microorganisms were sampled using an SAS Super 100 Air Sampler in three cotton farms during cotton harvesting, and colonies were counted and converted to airborne concentrations. Genomic DNA was extracted from air samples using a PowerSoil® DNA Isolation Kit. A series of comparative critical threshold (2−ΔΔCT) real-time PCR was used to quantify targeted bacterial (16S rRNA) genes and major ARGs. Two N95 facepiece respirator models (cup-shaped and pleated) were evaluated for their protection against culturable bacteria and fungi, total microbial load in terms of surface ATP levels, and ARGs using a field experimental setup. Overall, culturable microbial exposure levels ranged between 103 and 104 CFU/m3 during cotton harvesting, which was lower when compared with bioaerosol loads reported earlier during other types of grain harvesting. The findings suggested that cotton harvesting works can release antibiotic resistance genes in farm air and the highest abundance was observed for phenicol. Field experimental data suggested that tested N95 respirators did not provide desirable >95% protections against culturable microorganisms, the total microbial load, and ARGs during cotton harvesting.

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The fexA gene in Campylobacter: whether the spread has occurred among various hosts in eastern China

Huang,

Chen,

Zang

et al. 2024

Journal of Global Antimicrobial Resistance

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Prevalence of the phenicol resistance gene fexA in Campylobacter isolated from the poultry supply chain

Cited by 4 publications

References 39 publications

Transmission of linezolid-resistant Enterococcus isolates carrying optrA and poxtA genes in slaughterhouses

Transmission of linezolid-resistant Enterococcus isolates carrying optrA and poxtA genes in slaughterhouses

Exposure Levels of Airborne Fungi, Bacteria, and Antibiotic Resistance Genes in Cotton Farms during Cotton Harvesting and Evaluations of N95 Respirators against These Bioaerosols

The fexA gene in Campylobacter: whether the spread has occurred among various hosts in eastern China

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