Prevalence of the Panton-Valentine Leukocidin Gene in Clinical Isolates of <i>Staphylococcus aureus</i> Isolated From Hospitals the Ilam Province of Iran

Rostamzad, Arman; Rostamneia, Nabi

doi:10.17795/ajcmi-32691

Cited by 4 publications

(4 citation statements)

References 20 publications

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“…These data are compatible with other previous findings which showed that 56% of 24 years old female patients carried pvl positive Methicillin-resistant S. aureus MRSA strains [13]. These findings agreed with those of earlier studies [14,15] which illustrated that luk-pv gene appeared with in 40.62% of clinical samples from younger age group (less than 15 years old). In addition , a significant presence of PVL age group was detected in subjects with of less than 15 years in comparison to older patients.…”

Section: Detection Of Plv Gene Using Pcrsupporting

confidence: 93%

Epidemiological Study on the Prevalence of Staphylococcus aureus PVL Gene Among Healthy Community in Al- Karkh and Al -Rusaffa Districts Baghdad, Iraq

Jawad

Mohammed

al-Hashimi

2022

eijs

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Five hundred nasal swabs were taken from normal medical staff and public in the city of Baghdad. Several identification parameters were used to recognize the bacterial isolates. S. aureus isolations form nasal swabs were identified using morphology and VITECK 2 system. Polymerase chain reaction (PCR) was employed to determine PVL (Panton–Valentine leukocidin ) gene in S. aureus. The data showed no significant evidence on the relationship between PVL gene presence and gender and age of the studied groups. There was no relation between the prevalence of PVL gene in the age groups of 21-30 years (p=0.328) and 31-40 years (p=0.682). The results showed that 38.4% and 37.5% S. aureus isolates were isolated from nurses working at Baghdad Teaching Hospital and AL-Yarmouk Teaching Hospital, respectively, while, doctors, pharmacists, and radiology staff were negative (0.00%). There was no significant difference between hospital staff and community in AL-Karkh (p=0.838) and in AL-Rusafa (p=0.118).

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Section: Detection Of Plv Gene Using Pcrsupporting

confidence: 93%

Epidemiological Study on the Prevalence of Staphylococcus aureus PVL Gene Among Healthy Community in Al- Karkh and Al -Rusaffa Districts Baghdad, Iraq

Jawad

Mohammed

al-Hashimi

2022

eijs

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“…As for vancomycin, most of them were sensitive to this antibiotic by 90%, whereas the percentage of sensitivity to inhibitors of basic metabolic substances was trimethoprim sulfamethoxazole and trimethoprim 95% incompatible with Rostamzad and Rostamneia. [ 23 ] The reason for the sensitivity of some bacterial isolates to the antibiotics under study is that they were able to reach their targets efficiently and thus affect the target site according to the mechanism of action of each antibiotic.…”

Section: Resultsmentioning

confidence: 99%

Prevalence of panton–Valentine leukocidin and toxic shock syndrome toxin-1 genes in methicillin-resistant Staphylococcus aureus isolated from nose of restaurant workers in Kirkuk city

Mahdi¹

2023

J Adv Pharm Technol Res

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“…Johnsson et al [ 39 ] detected pvl gene in one isolate (1%) from 65 patients with S. aureus bacteraemia, in two (2.19%) isolates from 91 patients with cutaneous infections, and in four (7.27%) isolates from 55 patients with respiratory tract infections. Rostamzad et al [ 40 ] reported that out of thirty-two MRSA isolates, thirteen isolates (40.62%) were positive for presence of the luk-pv gene from hospitals isolates. Higher prevalence of PVL among children (<14 years of age) was observed as compared to adults and old age group patients.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Panton-Valentine Leukocidin Gene among Community AcquiredStaphylococcus aureus: A Real-Time PCR Study

Karmakar

Jana

Dutta

et al. 2018

Journal of Pathogens

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Panton-Valentine leukocidin (luk-pv) is a cytotoxin that causes leukocyte destruction and tissue necrosis. The aim of this study was to determine the prevalence of the pv1, mecA, and nuc genes in Staphylococcus aureus isolates obtained from anterior nares and superficial infection sites of skin in a slum population of West Bengal, India. Expression level of pv1 gene was also analysed. Twenty-two S. aureus strains were isolated, and phenotype and genotype specific examinations for S. aureus isolates were carried out. Molecular identification was done by PCR using species-specific 16S rRNA primer pairs and finally 22 isolates were found to be positive as S. aureus. The antibiotic responsiveness of all these isolates and the minimum inhibitory concentration (MIC) of MRSA isolates were determined using the broth dilution method with vancomycin. Antibiogram analysis of isolated S. aureus strains with respect to different antimicrobial agents revealed antibiotic resistance ranging from 27 to 91%. The results of MIC for vancomycin showed 95% of strains to be VSSA and 5% to be VISA. 68% isolates were resistant to methicillin. All the isolates were subjected to detection of pv1, mecA, and nuc genes, and 9%, 68%, and 27% were found to harbour pvl, mecA, and nuc genes, respectively. All the MRSA strains produced high to moderate levels of biofilm. pvl gene expression was carried out in vitro by Real-Time PCR. The low ∆Ct value (0.493) was indicative of high expression of pvl in one S. aureus strain. Thus, detection of pvl gene in community acquired S. aureus indicates the emergence of pathogenic S. aureus in community setup in the studied region. The existing exploration is extremely imperative and informative for the high level multi-drug resistant S. aureus infections inclusive of MRSA.

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Prevalence of the Panton-Valentine Leukocidin Gene in Clinical Isolates of Staphylococcus aureus Isolated From Hospitals the Ilam Province of Iran

Cited by 4 publications

References 20 publications

Epidemiological Study on the Prevalence of Staphylococcus aureus PVL Gene Among Healthy Community in Al- Karkh and Al -Rusaffa Districts Baghdad, Iraq

Epidemiological Study on the Prevalence of Staphylococcus aureus PVL Gene Among Healthy Community in Al- Karkh and Al -Rusaffa Districts Baghdad, Iraq

Prevalence of panton–Valentine leukocidin and toxic shock syndrome toxin-1 genes in methicillin-resistant Staphylococcus aureus isolated from nose of restaurant workers in Kirkuk city

Prevalence of Panton-Valentine Leukocidin Gene among Community AcquiredStaphylococcus aureus: A Real-Time PCR Study

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