Prevalence of specific and phylogenetically closely related genotypes in the population of Candida albicans associated with genital candidiasis in China

Ge, Shengxiang; Xie, Jing; Xu, Jianping; Li, Juan; Li, Dongming; Zong, Liu; Zheng, Yangfan; Bai, Feng‐Yan

doi:10.1016/j.fgb.2011.10.006

Cited by 39 publications

(42 citation statements)

References 52 publications

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“…1, Table S1). This is a striking difference from a previous report that indicated C. albicans isolates associated with genital candidiasis in China were principally concentrated in clade 1 (Ge et al, 2012). It is speculated that the much more diversified nature of body sites from which C. albicans isolates obtained might account for this difference.…”

Section: Discussioncontrasting

confidence: 88%

Genetic and phenotypic characterization of Candida albicans strains isolated from infectious disease patients in Shanghai

et al. 2015

Journal of Medical Microbiology

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Candida albicans, as an opportunistic pathogen, can cause superficial and life-threatening candidiasis in immunocompromised individuals. The formation of surface-associated biofilms and the appearance of drug resistance pose a significant challenge for clinical intervention. In this study, a total of 104 hospital-acquired C. alibcans clinical isolates were collected from sterile sites and mucosal lesions of 92 infectious disease patients in the Shanghai Public Health Clinical Center and analysed. The resistance rates to fluconazole, itraconazole and voriconazole were 12.5 %, 15.4 % and 11.5 % respectively. Multilocus sequence typing (MLST) analysis identified 63 diploid sequence types (DSTs) with a decentralized phylogeny, of which 37 DSTs (58.7 %) had not been reported in the online MLST database. Loss of heterozygosity was observed in ACC1 and ADP1 sequences obtained from six sequential isolates from a patient receiving antifungal treatment, which exemplified the effect of microevolution on C. albicans genetic alterations. Biofilm formation capability, an important virulence trait of C. albicans, was variable among strains isolated from different anatomical sites (P50.0302) and affected by genotypes (P50.0185). The mRNA levels of the azole antifungal target ERG11 gene and efflux pump genes (CDR1, CDR2 and MDR1) were detected in 9-18.1 % of azole-resistant and susceptible-dose dependent (S-DD) isolates. Twelve mutations encoding distinct amino acid substitutions in ERG11 were found in azoleresistant and S-DD isolates. Among them, A114S, Y132H and Y257H substitution in the ERG11 gene may be primarily related to azole resistance. Taken together, we observed a high level of diversity within C. albicans isolates. Multiple inter-related underlying mechanisms, including genetic and environmental factors, may account for high surface adhesion or azole resistance in clinical C. albicans infections.Abbreviations: AAT1, aspartate aminotransferase; ACC1, Acetyl-coenzyme A carboxylase; ACT1, actin; ADP1, ATP-dependent permease; ALS, hyphaspecific surface protein; BSI, nosocomial bloodstream infection; CDR1, CDR2, ATP-dependent efflux pumps; CLSI, Clinical and Laboratory Standard Institute; DST, diploid sequence type; ERG11, cytochrome P450 14a-demethylase; HWP1, hypha-specific cell wall protein; MDR1, major facilitator pump; MLST, multilocus sequence typing; MPI, mannose phosphate isomerase; SAP, secreted aspartyl protease; S-DD, susceptible-dose dependent; SYA1, alanyl-RNA synthetase; VPS13, vacuolar protein sorting protein; XTT, 2,3-bis-(2-methoxy-4-nitro-5-sulphenyl)-2H-tetrazolium-5-carboxanilide; YPD, yeast extract, peptone, dextrose medium; ZWF1b, glucose-6-phosphate dehydrogenase.

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Section: Discussioncontrasting

confidence: 88%

Genetic and phenotypic characterization of Candida albicans strains isolated from infectious disease patients in Shanghai

et al. 2015

Journal of Medical Microbiology

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“…Their data suggest a great likelihood of C. albicans person-to-person transmission, and the effect of global human travel is likely to reduce geographically separated evolutionary trends [23]. According to the literature, the geographic specificity that has been proposed for the different clades should be reconsidered [20], [23], [30], [32], [35], [48]–[51]. In the web-based database, the strains in clades 2, 6, and 10 have not been reported in the Greater China area.…”

Section: Discussionmentioning

confidence: 99%

Particular Candida albicans Strains in the Digestive Tract of Dyspeptic Patients, Identified by Multilocus Sequence Typing

Gong

Zheng²,

Jin

et al. 2012

PLoS ONE

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Background Candida albicans is a human commensal that is also responsible for chronic gastritis and peptic ulcerous disease. Little is known about the genetic profiles of the C. albicans strains in the digestive tract of dyspeptic patients. The aim of this study was to evaluate the prevalence, diversity, and genetic profiles among C. albicans isolates recovered from natural colonization of the digestive tract in the dyspeptic patients.Methods and FindingsOral swab samples (n = 111) and gastric mucosa samples (n = 102) were obtained from a group of patients who presented dyspeptic symptoms or ulcer complaints. Oral swab samples (n = 162) were also obtained from healthy volunteers. C. albicans isolates were characterized and analyzed by multilocus sequence typing. The prevalence of Candida spp. in the oral samples was not significantly different between the dyspeptic group and the healthy group (36.0%, 40/111 vs. 29.6%, 48/162; P > 0.05). However, there were significant differences between the groups in the distribution of species isolated and the genotypes of the C. albicans isolates. C. albicans was isolated from 97.8% of the Candida-positive subjects in the dyspeptic group, but from only 56.3% in the healthy group (P < 0.001). DST1593 was the dominant C. albicans genotype from the digestive tract of the dyspeptic group (60%, 27/45), but not the healthy group (14.8%, 4/27) (P < 0.001).ConclusionsOur data suggest a possible link between particular C. albicans strain genotypes and the host microenvironment. Positivity for particular C. albicans genotypes could signify susceptibility to dyspepsia.

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“…The welldifferentiated, cryptic species, C. albicans and C. dubliniensis showed differences in virulence (Gilfillan et al 1998). Within C. albicans in China, it has been reported that different genotypes associate with different phenotypes of disease presentation and antifungal resistance (Bai 2014), and that his association can involve clades of pathogenic strains that are geographically distinct (Ge et al 2012). European C. albicans has also shown association between genotype and antifungal resistance, as well as cellsurface repeats, but not pathogenicity (Odds 2010).…”

Section: Population Geneticsmentioning

confidence: 99%

Evolutionary Perspectives on Human Fungal Pathogens

Taylor

2014

Cold Spring Harb Perspect Med

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Prevalence of specific and phylogenetically closely related genotypes in the population of Candida albicans associated with genital candidiasis in China

Cited by 39 publications

References 52 publications

Genetic and phenotypic characterization of Candida albicans strains isolated from infectious disease patients in Shanghai

Genetic and phenotypic characterization of Candida albicans strains isolated from infectious disease patients in Shanghai

Particular Candida albicans Strains in the Digestive Tract of Dyspeptic Patients, Identified by Multilocus Sequence Typing

Evolutionary Perspectives on Human Fungal Pathogens

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