Particular Candida albicans Strains in the Digestive Tract of Dyspeptic Patients, Identified by Multilocus Sequence Typing

Gong, Yanfen; Zheng, Jian-Ling; Jin, Bo; Zhuo, Dexiang; Zhu-qing, Huang; Qi, He; Zhang, Wei; Duan, Wei; Fu, Ji-Ting; Chui-jie, Wang; Mao, Zebin

doi:10.1371/journal.pone.0035311

Cited by 26 publications

(20 citation statements)

References 51 publications

(29 reference statements)

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“…Allelic concatenated nucleic acid sequences from 25 haemodialysis patient isolates, 47 control subject isolates, and 3256 reference strains retrieved from an MLST database were phylogenetically analysed by UPGMA. Clade numbers were assigned according to reference 41 . Three clades (10, 13, and 14) were not represented in isolates from either group.…”

Section: Resultsmentioning

confidence: 99%

“…Similarly, these clades contain the fewest strains in the global C. albicans MLST database. No isolates in Clade 13 were found in studies from Chinese regions such as Beijing 37 , Chengdu 38 , Shanghai 39 , 40 , Shenyang 41 , and Taiwan 42 – 44 . Several earlier studies found strong evidence for a geographic influence in the low representation of some clades in certain regions of the world.…”

Section: Discussionmentioning

confidence: 99%

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Multilocus sequence typing of Candida albicans isolates from the oral cavities of patients undergoing haemodialysis

Gong

Jin

Qi³

et al. 2018

Sci Rep

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This study evaluates the prevalence, diversity, and genetic profiles of Candida albicans isolates recovered from the oral cavities of haemodialysis patients. Oral swab samples were obtained from haemodialysis patients (n = 126) and healthy control subjects (n = 233) and Candida species were characterised. There was no significant difference between the haemodialysis and control groups in the prevalence of yeast carriers (23.6% vs. 31.0%, respectively) or C. albicans carriers (19.8% vs. 21.0%, respectively). C. albicans was the most populous species in both cohorts, followed by C. parapsilosis. C. parapsilosis and C. glabrata were more prevalent in the haemodialysis group than in the control group (C. parapsilosis 5.6% vs. 0.9% and C. glabrata 3.2% vs. 0.4%, respectively; P < 0.05). C. albicans isolates were analysed by multilocus sequence typing and the results were used to construct a phylogenetic tree. Most haemodialysis isolates were placed into Clade 4 (20.0%) and Clade 19 (16.0%) and most control isolates into Clade 8 (17%) and Clade 4 (14.9%). Differences in the strain abundance in each clade were not statistically significant between the two groups. Moreover, there was no significant association between the health status or diagnosis and either the sequence types or clades.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Multilocus sequence typing of Candida albicans isolates from the oral cavities of patients undergoing haemodialysis

Gong

Jin

Qi³

et al. 2018

Sci Rep

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“…albicans isolates in the digestive tract of dyspeptic patients revealed a possible link between particular C . albicans genotypes and the host microenvironment [ 12 ]. For example, the C .…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, a study of C . albicans sampled from the digestive tract of dyspeptic patients was conducted in Shenyang [ 12 ]. However, no epidemiological study of C .…”

Section: Introductionmentioning

confidence: 99%

Multilocus Sequence Typing of Pathogenic Candida albicans Isolates Collected from a Teaching Hospital in Shanghai, China: A Molecular Epidemiology Study

Luò

et al. 2015

PLoS ONE

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Molecular typing of Candida albicans is important for studying the population structure and epidemiology of this opportunistic yeast, such as population dynamics, nosocomial infections, multiple infections and microevolution. The genetic diversity of C. albicans has been rarely studied in China. In the present study, multilocus sequence typing (MLST) was used to characterize the genetic diversity and population structure of 62 C. albicans isolates collected from 40 patients from Huashan Hospital in Shanghai, China. A total of 50 diploid sequence types (DSTs) were identified in the 62 C. albicans isolates, with 41 newly identified DSTs. Based on cluster analysis, the 62 isolates were classified into nine existing clades and two new clades (namely clades New 1 and New 2). The majority of the isolates were clustered into three clades, clade 6 (37.5%), clade 1 (15.0%) and clade 17 (15.0%). Isolates of clade New 2 were specifically identified in East Asia. We identified three cases of potential nosocomial transmission based on association analysis between patients’ clinical data and the genotypes of corresponding isolates. Finally, by analyzing the genotypes of serial isolates we further demonstrated that the microevolution of C. albicans was due to loss of heterozygosity. Our study represents the first molecular typing of C. albicans in eastern China, and we confirmed that MLST is a useful tool for studying the epidemiology and evolution of C. albicans.

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“…26,27 Furthermore, Candida albicans can grow well in highly acidic environments, 28 and some genotypes may increase the severity of gastric mucosal lesions. 29 Recently, a study using bacterial and fungal DNA co-sequencing analysis obtained 19-81 fungal genus-level operational taxonomic units in gastric juice, but only identified two fungal genera including Candida and Phialemonium. 30 Nevertheless, it is still uncertain whether gastric fungi or bacteria other than Helicobacter pylori participate in the pathogenesis of gastric diseases (e.g.…”

Section: Nature/classification Of Fungi In the Human Gi Tractmentioning

confidence: 99%

Review article: fungal microbiota and digestive diseases

Wang

Yang

Stefka

et al. 2014

Aliment Pharmacol Ther

105

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Summary Background The role of the fungal microbiota in digestive diseases is poorly defined, but is becoming better understood due to advances in metagenomics. Aim To review the gastrointestinal fungal microbiota and its relationship with digestive diseases. Methods Search of the literature using PubMed and MEDLINE databases. Subject headings including ‘fungal‐bacterial interactions’, ‘mycotoxins’, ‘immunity to fungi’, ‘fungal infection’, ‘fungal microbiota’, ‘mycobiome’ and ‘digestive diseases’ were used. Results The fungal microbiota is an integral part of the gastrointestinal microecosystem with up to 106 microorganisms per gram of faeces. Next‐generation sequencing of the fungal 18S rRNA gene has allowed better characterisation of the gastrointestinal mycobiome. Numerous interactions between fungi and bacteria and the complex immune response to gastrointestinal commensal or pathogenic fungi all impact on the pathophysiology of inflammatory bowel disease and other gastrointestinal inflammatory entities such as peptic ulcers. Mycotoxins generated as fungal metabolites contribute to disturbances of gastrointestinal barrier and immune functions and are associated with chronic intestinal inflammatory conditions as well as hepatocellular and oesophagogastric cancer. Systemic and gastrointestinal disease can also lead to secondary fungal infections. Fungal genomic databases and methodologies need to be further developed and will allow a much better understanding of the diversity and function of the mycobiome in gastrointestinal inflammation, tumourigenesis, liver cirrhosis and transplantation, and its alteration as a consequence of antibiotic therapy and chemotherapy. Conclusions The fungal microbiota and its metabolites impact gastrointestinal function and contribute to the pathogenesis of digestive diseases. Further metagenomic analyses of the gastrointestinal mycobiome in health and disease is needed.

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Particular Candida albicans Strains in the Digestive Tract of Dyspeptic Patients, Identified by Multilocus Sequence Typing

Cited by 26 publications

References 51 publications

Multilocus sequence typing of Candida albicans isolates from the oral cavities of patients undergoing haemodialysis

Multilocus sequence typing of Candida albicans isolates from the oral cavities of patients undergoing haemodialysis

Multilocus Sequence Typing of Pathogenic Candida albicans Isolates Collected from a Teaching Hospital in Shanghai, China: A Molecular Epidemiology Study

Review article: fungal microbiota and digestive diseases

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