Multilocus sequence typing of Candida albicans isolates from the oral cavities of patients undergoing haemodialysis

Gong, Yanfen; Jin, Bo; Qi, He; Zhang, Rong; Zhang, Xiuying; Yuan, Ping; Zhao, Tong-Xiang; Geng, Xing-Hua; Zhang, Min; Zheng, Jian-Ling

doi:10.1038/s41598-018-34565-7

Cited by 9 publications

(16 citation statements)

References 68 publications

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“…Of note, 4 of the 6 new DSTs were recovered from human patients, whereas the majority of animal isolates belonged to previously described MLST genotypes (19/24; 79%) (Table 1 ). In order to reveal the phylogenetic relationship between isolates, we performed cluster analysis of isolates included in the present study and two reference DSTs from each previously assigned clades determined by UPGMA analysis [ 10 ]. The 30 C. albicans isolates grouped into 8 clades.…”

Section: Resultsmentioning

confidence: 99%

“…Genotyping strains within a microbial species on the basis of DNA sequences at multiple loci has greatly advanced study of the epidemiology and evolutionary phylogenetic of many fungal pathogens including C. albicans [ 5 – 8 ]. At the population level, molecular typing has revealed 19 clades of C. albicans strains so far [ 9 , 10 ]. Some of these clades seem to exhibit geographical enrichment or phenotype specificities, however, no correlation between clade assignment and the ability of strains to cause different forms of infection or host specificity has been established yet [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Diversity and Genetic Relatedness of Candida albicans Isolates from Birds in Hungary

et al. 2021

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The molecular epidemiology of Candida albicans infections in animals has been rarely studied. In this study, multilocus sequence typing was used to characterise the genetic diversity and population structure of 24 avian origin C. albicans isolates collected from different birds with candidiasis and compared to human isolates. Fourteen diploid sequence types (DSTs) including six new DSTs were determined. Cluster analysis revealed that isolates grouped into 8 clades. Bird isolates mainly belonged to minor clades and Clade 15 with DST 172 was the most common (11 isolates; 45.8%). The remaining isolates were clustered into Clade 7 (5 isolates; 20.8%), Clade 10 (4 isolates; 16.6%), Clade 8 (2 isolates; 8.3%), Clade 4 (1 isolate; 4.2%) and Clade 16 (1 isolate; 4.2%). Unweighted pair group method with arithmetic averages (UPGMA) and eBURST analyses showed that the genetic construction of avian origin C. albicans population is fairly diverse. Although species-specific lineages were not found, some degree of separation in the evolution of bird and human strains could be observed.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Diversity and Genetic Relatedness of Candida albicans Isolates from Birds in Hungary

et al. 2021

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“…Bootstrap values of ≥75% were defined as statistically significant. Finally, clade numbers were assigned according to previous publications (Odds et al, 2007; Tsai et al, 2015; Wu et al, 2015; Gong et al, 2018) or by using the eBURST method as described previously (Odds et al, 2007). DSTs from clade numbers that were not presented in our strains were also included as reference DSTs for UPGMA and NJ analysis (Figure 1).…”

Section: Methodsmentioning

confidence: 99%

“…Multilocus sequence typing (MLST) has been widely used to study the molecular characterization of C. albicans based on the sequences of seven housekeeping genes ( AAT1a , ACC1 , ADP1 , MPI1b , SYA1 , VPS13 , and ZWF1b ) (Bougnoux et al, 2003; Afsarian et al, 2015). This method has identified 19 clades worldwide (Shin et al, 2011; Gong et al, 2018). A 2007 global study of 1,391 C. albicans strains showed that clade 1 was the most common (33.6%) (Odds et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

A Predominance of Clade 17 Candida albicans Isolated From Hemocultures in a Tertiary Care Hospital in Thailand

et al. 2019

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Candida albicans is one of the most common human fungal pathogens. Candidemia has significant mortality globally. No epidemiological study of C . albicans based on multilocus sequence typing (MLST) has been conducted in Thailand. Therefore, MLST was used to study the molecular epidemiology of C . albicans blood strains in a large Thai teaching hospital. In vitro virulence phenotypes and antifungal susceptibility testing by broth microdilution were also conducted. Forty-six C . albicans blood strains from 37 patients were collected from the Department of Microbiology, Siriraj Hospital, in 2016 and 2017. Most patients (71.8%) were more than 60 years old, and the case fatality rate was 54.8%. The male-to-female ratio was 5:3. Thirty-four diploid sequence types (DSTs), including six new DSTs, were identified, with DST2514 (8.7%) and DST2876 (8.7%) as the most common DSTs. Strains were clustered into nine clades. Unlike other studies of C. albicans blood strains in Asia, clade 17 was the most common (13 strains, 28.3%). Sequential allelic changes were evident in sequential strains from one patient. All strains produced phospholipase and hemolysin, while none produced proteinase. The ability to form biofilm was found in 82.6% of the strains. Clade 17 strains showed significantly stronger hemolytic activity than non–clade 17 strains (69.2% versus 27.3%; p = 0.022). However, no significant association existed between clades and patient mortalities. All were susceptible or wild type to anidulafungin (MIC range = 0.015–0.12 and GM = 0.030), micafungin (MIC range = ≤ 0.008–0.015 and GM = 0.008), caspofungin (MIC range = 0.008–0.12 and GM = 0.036), and amphotericin B (MIC range = 0.25–0.5 and GM = 0.381). Only one strain was resistant to voriconazole (MIC range = ≤ 0.008 to ≥ 8 and GM = 0.010) and fluconazole (MIC range = 0.12–16 and GM = 0.398). In conclusion, a high prevalence of clade 17 C. albicans blood strains was found in Thailand, in contrast to other Asian countries. This unique finding might be explained by the strong hemolytic activity that is required for bloodstream infection of C. albicans.

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“…[10][11][12] This method has identified 19 clades plus singletons worldwide. 13,14 In this study, we used molecular techniques and cluster analysis to analyse the distribution of infected agents across China.…”

Section: Introductionmentioning

confidence: 99%

A prospective study on vulvovaginal candidiasis: multicentre molecular epidemiology of pathogenic yeasts in China

Song

Kan

Pang

et al. 2021

Acad Dermatol Venereol

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Background Vulvovaginal candidiasis (VVC) is frequent in women of reproductive age, but very limited data are available on the epidemiology in cases of VVC in China.Objectives The current study has been conducted to reveal the prevalence, species distribution of yeast causing VVC and molecular genetics of Candida albicans in China.Methods Vaginal swabs were collected from 543 VVC outpatients recruited in 12 hospitals in China between September 2017 and March 2018. They were preliminarily incubated on Sabouraud dextrose agar and then positive subjects of which were then transmitted to our institute for further identification. CHROMagar TM was used to isolate Candida species, and all isolates were finally identified by DNA sequencing. Multilocus sequence typing (MLST) was used to analyse phylogenetic relationships of the various C. albicans isolates. ResultsEleven different yeast species were identified in 543 isolates, among which C. albicans (84.7%) was the most frequent, followed by C. glabrata (8.7%). We obtained 117 unique diploid sequence types from 451 clinical C. albicans isolates and 92 isolates (20.4%) belonged to a New Clade. All the strains appearing in the New Clade were from northern China and they were isolated from non-recurrent VVC.Conclusions Our findings suggest that C. albicans are still the main cause of VVC in China and the majority of C. albicans isolates belongs to Clade 1 with DST 79 and DST 45 being two most common. Moreover, the New Clade revealed in our study seems to be specific to northern China.

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Multilocus sequence typing of Candida albicans isolates from the oral cavities of patients undergoing haemodialysis

Cited by 9 publications

References 68 publications

Molecular Diversity and Genetic Relatedness of Candida albicans Isolates from Birds in Hungary

Molecular Diversity and Genetic Relatedness of Candida albicans Isolates from Birds in Hungary

A Predominance of Clade 17 Candida albicans Isolated From Hemocultures in a Tertiary Care Hospital in Thailand

A prospective study on vulvovaginal candidiasis: multicentre molecular epidemiology of pathogenic yeasts in China

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