Prevalence of some virulence genes and antibiotic susceptibility pattern of Pseudomonas aeruginosa isolated from different clinical specimens

Chand, Yamuna; Khanal, Santosh; Panta, Om Prakash; Shrestha, Dipendra; Khadka, Dhruba Kumar; Poudel, Pramod

doi:10.21203/rs.3.rs-24044/v1

Cited by 4 publications

(7 citation statements)

References 10 publications

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“…showed resistance to ciprofloxacin that is comparable to studies of Anil and Shahid (2013), and Ramana and Chaudhury (2012) that showed 27.59 % and 39% resistant to ciprofloxacin, respectively. The result is also comparable to recent studies of Agbo et al (2020), Chand et al (2020) and Kumar et al (2020) which showed 21.68 %, 31.03 %, and 35.55 % resistant to ciprofloxacin, respectively, suggesting it could be the regular regimen for Pseudomonas spp infection.…”

Section: Discussionsupporting

confidence: 91%

“…These findings are also similar to the findings of Chand et al (2020) indicating higher sensitivity of gentamycin with a resistant rate of only 20.68 %. The result is not consistent with the findings of Agbo et al (2020) and Motbainor et al (2020) since they reported that for Pseudomonas the resistance rate against gentamycin was high i.e.…”

Section: Discussionsupporting

confidence: 90%

“…The present study also showed a high rate of resistance of isolates to cefixime (96.4 %) and ceftriaxone (57.1 %). These high values of resistance are comparable to the reports of Chand et al (2020) and Motbainor et al (2020) that showed 100 % resistance to both cefixime and ceftriaxone. These findings are also similar to the studies of Anil and Shahid (2013) that revealed a higher resistance rate (68.96 %) to ceftriaxone.…”

Section: Discussionsupporting

confidence: 88%

“…Low rate of resistance to piperacillin/tazobactam (11.1 % and 19.4 %) was reported by Kumar et al (2020) and Chand et al (2020), respectively. Similarly, low rate of resistance to meropenem was reported by Agbo et al (2020).…”

Section: Discussionmentioning

confidence: 82%

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Antimicrobial Susceptibility Patterns of Pseudomonas Species Isolated from Various Clinical Samples at a Tertiary Care Hospital

Gyawali

Khadka

Shrestha

et al. 2020

J. Inst. Sci. Tech.

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Considerable increase in the prevalence and multidrug-resistant (MDR) Pseudomonas has been observed with towering morbidity and mortality. As a consequence of the haphazard use of antimicrobials, the spread of antimicrobial resistance is now a global issue. This study aimed to access the distribution rate and antibiotic susceptibility patterns of Pseudomonas species isolated from various clinical specimens in Kathmandu Model Hospital, Nepal. During the study period, 1252 samples were collected, cultured and the organism was isolated and identified. The antimicrobial susceptibility testing was done using the modified Kirby-Bauer disc diffusion method as per CLSI guidelines. Out of 1252 samples, 28 clinical isolates of Pseudomonas species were isolated. The highest number of Pseudomonas spp. was isolated from swab samples that included pus, ear, and wound (46.4 %). Pseudomonas spp. demonstrated marked resistance against cefixime (96.4 %) and showed higher sensitivity to piperacillin/tazobactam (92.9 %). The result showed pus, wound exudates, ear discharges samples exhibit Pseudomonas as common etiology of infection. Pseudomonas spp. demonstrated highest sensitivity against piperacillin/tazobactam, amikacin, meropenem, gentamycin. The steady resistance of Pseudomonas spp. to most of the antibiotics, necessitates these drugs to be confined to extreme infections and hospital intensive care units to circumvent the speedy emergence of resistant strains.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 82%

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Antimicrobial Susceptibility Patterns of Pseudomonas Species Isolated from Various Clinical Samples at a Tertiary Care Hospital

Gyawali

Khadka

Shrestha

et al. 2020

J. Inst. Sci. Tech.

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“…Yamuna Chand, Sujan Khadka, and Sanjeep Sapkota share the first authorship. A preprint of the current study has previously been published [50].…”

Section: Abbreviationsmentioning

confidence: 99%

Clinical Specimens are the Pool of Multidrug- resistant Pseudomonas aeruginosa Harbouring oprL and toxA Virulence Genes: Findings from a Tertiary Hospital of Nepal

Chand

Khadka

Sharma

et al. 2021

Emergency Medicine International

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The multidrug- or extensively drug-resistant (MDR/XDR) Pseudomonas aeruginosa carrying some virulence genes has become a global public health threat. However, in Nepal, there is no existing report showing the prevalence of oprL and toxA virulence genes among the clinical isolates of P. aeruginosa. Therefore, this study was conducted for the first time in the country to detect the virulence genes (oprL and toxA) and antibiotic susceptibility pattern of P. aeruginosa. A total of 7,898 clinical specimens were investigated following the standard microbiological procedures. The antibiotic susceptibility testing was examined by the modified disc diffusion method, and virulence genes oprL and toxA of P. aeruginosa were assessed using multiplex PCR. Among the analyzed specimens, 87 isolates were identified to be P. aeruginosa of which 38 (43.68%) isolates were reported as MDR. A higher ratio of P. aeruginosa was detected from urine samples 40 (45.98%), outpatients’ specimens 63 (72.4%), and in patients of the age group of 60–79 years 36 (41.37%). P. aeruginosa was more prevalent in males 56 (64.36%) than in female patients 31 (35.63%). Polymyxin (83.90%) was the most effective antibiotic. P. aeruginosa (100%) isolates harboured the oprL gene, while 95.4% of isolates were positive for the toxA gene. Identification of virulence genes such as oprL and toxA carrying isolates along with the multidrug resistance warrants the need for strategic interventions to prevent the emergence and spread of antimicrobial resistance (AMR). The findings could assist in increasing awareness about antibiotic resistance and suggest the judicious prescription of antibiotics to treat the patients in clinical settings of Nepal.

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Emerging Patterns in Antimicrobial Susceptibility Profiles of Pseudomonas SPP- Hospital Based Study

Ikram¹,

Inam²,

Shamim³

et al. 2023

PJMHS

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Background: There is an emerging trend of pseudomonas infections in immune-compromised patients, specifically in hospital settings. The flagship member of this family is Pseudomonas aeruginosa, which is a major infectious agent. This study assessed the distribution and susceptibility patterns of Pseudomonas species isolated from various specimens as a part of surveillance program, in order to devise antibiograms. Aim: To determine frequency & antimicrobial sensitivities of Pseudomonas species in a tertiary care hospital from Lahore. Study design: Retrospective, descriptive, cross sectional study Place & duration of study: Conducted in a tertiary care hospital in Lahore, from January 2021 to January 2022. Methods: Thirty two isolates of Pseudomonas species from different clinical specimens were isolated in microbiology section of a tertiary care hospital during a period of 13 months. MacConkey and blood agar were utilized for culturing of organisms. Gram staining, oxidase, and catalase testwere utilized for phenotypic characterization. Antibiotic susceptibility testing against anti-pseudomonal drugs was performed according to the Clinical and Laboratory Standards Institute guidelines (CLSI) 2021. Results: The inference drawn at the end of study was that, out of thirty two isolates of Pseudomonas species received and pus was the most common specimen (46.8%), followed by 18.75% from urine specimens. 62.5% of the pseudomonas species were obtained from male patients. The most affected age group was 40-60 years, followed by 1-20 years. The most sensitive options turned out to be Imipenem (65.6%) and Pipericillintazobactam (62.5%), followed by 59.3% sensitivity in Amikacin. The least sensitive options in the study isolates were Aztreonam (15.6%) and Ticarcillin clavulanic acid (25%). In 18% of pseudomonas species isolated from urine cultures, fosfomycin was 83.3%, whereas nitrofurantoin turned out to be 50% sensitive. Conclusions: The steadily rising resistance in Pseudomonas species againstavailable antibiotics optionsnecessitates their use inlife-threatening and hospital acquiredinfections. Keywords: Peudomonas, Flouroquinolones, Carbapenem

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Prevalence of some virulence genes and antibiotic susceptibility pattern of Pseudomonas aeruginosa isolated from different clinical specimens

Cited by 4 publications

References 10 publications

Antimicrobial Susceptibility Patterns of Pseudomonas Species Isolated from Various Clinical Samples at a Tertiary Care Hospital

Antimicrobial Susceptibility Patterns of Pseudomonas Species Isolated from Various Clinical Samples at a Tertiary Care Hospital

Clinical Specimens are the Pool of Multidrug- resistant Pseudomonas aeruginosa Harbouring oprL and toxA Virulence Genes: Findings from a Tertiary Hospital of Nepal

Emerging Patterns in Antimicrobial Susceptibility Profiles of Pseudomonas SPP- Hospital Based Study

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