Prevalence of Sensitization to<i>Anisakis simplex</i>Among Professionally Exposed Populations in Sicily

Mazzucco, Walter; Lacca, Guido; Cusimano, Rosanna; Provenzani, A; Costa, Antonella; Noto, A.M. Di; Massenti, Maria Fatima; Leto-Barone, Maria Stefania; Lorenzo, Gabriele Di; Vitale, Francesco

doi:10.1080/19338244.2011.578683

Cited by 25 publications

(33 citation statements)

References 21 publications

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“…was also related with occupational seafood allergy [40], induced by the contact of parasite allergens with skin or respiratory epithelium [45]. In this sense, Italian authors reported that Anisakis larvae represent a potential occupational risk in fishermen and workers assigned to fish processing and sale, with specific anti- Anisakis IgE detected in 20.2% of the studied population [46]. Similarly, recent research in Croatia described that chronic respiratory symptoms associated with occupational asthma were significantly dominant in fish processing workers compared to controls [47].…”

Section: Discussionmentioning

confidence: 94%

Anti-Anisakis IgE Seroprevalence in the Healthy Croatian Coastal Population and Associated Risk Factors

et al. 2014

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BackgroundThe main objective of the study was to determine the degree of sensitization to Anisakis spp. antigens in healthy coastal population of Dalmatia given the high thermally unprocessed fish intake rate present in this area, suggested as a significant risk factor for anisakiasis. We performed a monocenter, cross-sectional pilot study stratified by geographic area of residence, conducted at the County secondary healthcare provider Medicine-biochemical Laboratory in Split (Croatia), from November 2010 till December 2011, on 500 unpaid volunteer subjects undergoing routine blood analysis and belonging to the south coast of the Adriatic Sea.Methodology/Principal FindingsWe studied the IgE seroprevalence to Anisakis spp. Ani s l and Ani s 7 allergens by indirect ELISA in healthy subjects, which were selected at random in the region of Dalmatia (Southern Croatia), among islands, coastal urban and inland rural populations. In order to detect possible cross-reactivity to other human helminthes, serum samples were tested also for the presence of IgG antibodies to Ascaris lumbricoides and Toxocara canis. The overall and coastal Anisakis seroprevalences for the sampled population were 2% and 2.5%, respectively. The logistic univariate regression analysis confirmed that regarding anti-Anisakis IgE seroprevalence, raw fish intake, daily fish intake, homemade origin of fish dish and occupational contact (professional, artisanal or hobby contact with fishery or fish industry) were risk factors associated to Anisakis spp. sensitization, but neither of the variables was exclusive for a particular seropositive population. Also, a significant difference was observed between seropositive and seronegative subjects that had stated allergy or symptoms associated with allergy (atopic dermatitis, asthma or rhinitis) in their previous history.Conclusions/SignificanceBeing the first in Croatia, our study underlines the necessity of incorporating Anisakis spp. allergens in routine hypersensitivity testing of coastal population.

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Section: Discussionmentioning

confidence: 94%

Anti-Anisakis IgE Seroprevalence in the Healthy Croatian Coastal Population and Associated Risk Factors

et al. 2014

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“…The present study represents the first detection of species‐specific IgE‐mediated sensitivity to A. pegreffii , a zoonotic agent of gastric, intestinal and gastro‐allergic human anisakiasis in Italy . So far, several cases of generic Anisakis ‐IgE sensitivity in Italian patients have been reported; however, the specific antigens of A. pegreffii, recognized by IgE antibody responses were not previously characterized. This is the first time that A. pegreffii ES products have been detected and recognized as those antigens/allergens responsible for IgE‐ A. pegreffii sensitivity in the human sera.…”

Section: Discussionmentioning

confidence: 81%

“…Anisakis ‐specific IgE sensitization has been extensively reported during recent decades, mainly from Spain, Croatia, Morocco and Italy . However, most of the epidemiological studies used to assess anti‐ Anisakis ‐IgE sensitization and Anisakis allergy have been carried out based on IgE‐ Anisakis reactivity detected by ImmunoCAP (iCAP) .…”

Section: Introductionmentioning

confidence: 99%

“…However, most of the epidemiological studies used to assess anti‐ Anisakis ‐IgE sensitization and Anisakis allergy have been carried out based on IgE‐ Anisakis reactivity detected by ImmunoCAP (iCAP) . A high level of Anisakis ‐IgE hypersensitivity in Italian fishery products handlers, fishermen and consumers of fish has been also reported using the iCAP methodology . However, in these patients, the presence of larvae was not detected.…”

Section: Introductionmentioning

confidence: 99%

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IgE sensitization to Anisakis pegreffii in Italy: Comparison of two methods for the diagnosis of allergic anisakiasis

Mattiucci

Colantoni

Crisafi

et al. 2017

Parasite Immunology

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IgE sensitization to Anisakis pegreffii in Italian subjects suffering from gastro-allergic anisakiasis (GAA) (N=5), or showing chronic urticaria (CU+) after fish consumption (N=100), was investigated. A control group (N=5) was also included. IgE response was analysed by immunoblotting (WB) assay, using both excretory/secretory products (ESPs) and crude extract (CE) of A. pegreffii larvae. The results were compared with those achieved by the conventional immunological method for Anisakis allergy (ie, immunoCAP). Among the 110 subjects, 28 showed IgE positivity with both WB and iCAP methods; 13 proved IgE reactivity, in WB assay, to ESP antigens of A. pegreffii, here provisionally indicated as Ani s 1-like, Ani s 7-like, Ani s 13-like; only 15 sera have shown IgE-WB reaction to Ani s 7-like and Ani s 13-like. iCAP and WB exhibited a high concordance value (κ=1.00) when iCAP value was <0.35 (negative result) and >50.0 (positive result). In the sera samples recorded as positive to Anisakis allergy, Ani s 1-like was responsible for 46.4% of the sensitivity, while Ani s 7-like and Ani s 13-like for 100%. They could be considered as major antigens in the diagnosis of allergic anisakiasis caused by A. pegreffii.

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“…in the general population range from 0.4% to 10%,11,22,23,24 and can reach 20% depending on atopic status, occupational exposure, and frequency of raw seafood consumption 25. In a multicenter study in Italy, the sensitization rates differed markedly from 0.4% in inland residents to 12.7% in coastal residents, suggesting that difference in anchovy-eating habits between the 2 regions may have affected the sensitization rate 24.…”

Section: Discussionmentioning

confidence: 99%

Clinical Characteristics of Gastroallergic Anisakiasis and Diagnostic Implications of Immunologic Tests

Chung

Lee

2014

Allergy Asthma Immunol Res

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PurposeRecent studies have used the term "gastroallergic anisakiasis" to describe incidental gastrointestinal infection with Anisakis spp. larvae, proposed as a causative agent of food hypersensitivity. However, it is unknown whether this condition represents an independent disease entity distinguishable from acute gastric anisakiasis. To better understand the role of the allergic response in Anisakis infections we examined the clinical and immunological implications of Anisakis-specific IgE.MethodsA prospective study was performed in a geographic region where the consumption of raw seafood is common. Case subjects who had been clinically diagnosed with gastroallergic anisakiasis were selected, along with controls who frequently ate raw seafood but had never experienced gastroallergic anisakiasis-like symptoms. Clinical and immunological features were compared based on atopic status, sensitization rates to Anisakis, and serum titer of Anisakis-specific IgE.ResultsSeventeen case subjects and 135 controls were included in this study. The case subjects had experienced gastrointestinal symptoms after raw seafood ingestion, along with additional mucocutaneous, respiratory, or multisystemic symptoms. Case subjects were significantly sensitized to Anisakis excretory-secretory product and crude extract compared with controls (76.5% vs 19.3%, P<0.001, and 88.2% vs 30.3%, P<0.001, respectively). Anisakis-specific serum IgE titers were also significantly higher in case subjects than in controls. Both the results of skin prick tests and elevated Anisakis-specific IgE titers (>17.5 kU/L) were found to be reliable indicators for the diagnosis of gastroallergic anisakiasis.ConclusionsAmong patients presenting acute gastric anisakiasis-like symptoms, a diagnosis of gastroallergic anisakiasis may be strongly supported by a high Anisakis-specific IgE titer.

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Prevalence of Sensitization toAnisakis simplexAmong Professionally Exposed Populations in Sicily

Cited by 25 publications

References 21 publications

Anti-Anisakis IgE Seroprevalence in the Healthy Croatian Coastal Population and Associated Risk Factors

Anti-Anisakis IgE Seroprevalence in the Healthy Croatian Coastal Population and Associated Risk Factors

IgE sensitization to Anisakis pegreffii in Italy: Comparison of two methods for the diagnosis of allergic anisakiasis

Clinical Characteristics of Gastroallergic Anisakiasis and Diagnostic Implications of Immunologic Tests

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