Prevalence of Plasmid-Mediated Quinolone Resistance Determinants and OqxAB Efflux Pumps among Extended-Spectrum<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mi mathvariant="bold-italic">β</mml:mi></mml:mrow></mml:math>-Lactamase Producing<i>Klebsiella pneumoniae</i>Isolated from Patients with Nosocomial Urinary Tract Infection in Tehran, Iran

Goudarzi, Mehdi; Azad, Mehdi; Seyedjavadi, Sima Sadat

doi:10.1155/2015/518167

Cited by 30 publications

(27 citation statements)

References 38 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A substantially higher proportion of qepA and aac-(6 ′) -Ib was found among PMQR genes. This trend is in agreement with recent clinical surveillance results showing significantly higher occurrence of aac-(6 ′) -Ib-cr 56575859. Although no comprehensive comparison of PMQR levels in aquatic environments could be found in the literature, two recent investigations on bacteria isolated from aquatic environments suggested the occurrence of aac-(6 ′) -Ib-cr is indeed higher than qnr and qepA genes6061.…”

Section: Discussionsupporting

confidence: 90%

Bacterial plasmid-mediated quinolone resistance genes in aquatic environments in China

Yan

Liú

Wang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Emerging antimicrobial resistance is a major threat to human’s health in the 21st century. Understanding and combating this issue requires a full and unbiased assessment of the current status on the prevalence of antimicrobial resistance genes and their correlation with each other and bacterial groups. In aquatic environments that are known reservoirs for antimicrobial resistance genes, we were able to reach this goal on plasmid-mediated quinolone resistance (PMQR) genes that lead to resistance to quinolones and possibly also to the co-emergence of resistance to β-lactams. Novel findings were made that qepA and aac-(6′)-Ib genes that were previously regarded as similarly abundant with qnr genes are now dominant among PMQR genes in aquatic environments. Further statistical analysis suggested that the correlation between PMQR and β-lactam resistance genes in the environment is still weak, that the correlations between antimicrobial resistance genes could be weakened by sufficient wastewater treatment, and that the prevalence of PMQR has been implicated in environmental, pathogenic, predatory, anaerobic, and more importantly, human symbiotic bacteria. This work provides a comprehensive analysis of PMQR genes in aquatic environments in Jinan, China, and provides information with which combat with the antimicrobial resistance problem may be fought.

show abstract

Section: Discussionsupporting

confidence: 90%

Bacterial plasmid-mediated quinolone resistance genes in aquatic environments in China

Yan

Liú

Wang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Meanwhile, a study conducted in Iran has reported that almost all ESBL-producing strains were resistant to these antibiotics (23). Based on the findings, in the present study, the resistance of the ESBL-producing strains against aminoglycosides (68.3%) was higher than those reported by Goudarzi et al (46.35%), Shams et al (45%), and Eftekhar (36.7%) (10,13,22). Moreover, more than 50% of the ESBLproducing K. pneumonia isolates were resistant against fluoroquinolones, and the highest and lowest resistance was, respectively, found against nalidixic acid (65.4%) and gatifloxacin (40.4%).…”

Section: Discussionsupporting

confidence: 54%

“…However, PMQR determinants were found in 80.76% of ESBLproducing isolates. Based on the studies conducted in Iran, the prevalence of PMQR genes in K. pneumonia isolates ranges from 39.5 to 89.1% (10,13,26,27,29,30), while in other countries it ranges from 11.1 to 100% (e.g., South Korea, the United States, and Syria) (31)(32)(33). Generally, susceptibility to quinolones and aminoglycosides is lower in species harboring the acetyltransferase aac(6')-Ib-cr gene.…”

Section: Discussionmentioning

confidence: 99%

Frequency of qnr and aac(6’)Ib-cr Genes Among ESBL-Producing Klebsiella pneumoniae Strains Isolated from Burn Patients in Kermanshah, Iran

Vaziri

Afsharian

Mansouri

et al. 2020

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Assessment of bacteria such as Klebsiella pneumonia has shown that Plasmid-mediated quinolone resistance (PMQR) affects antibiotics resistance (e.g., quinolones). Objectives: We studied the prevalence of qnr and aac(6’)Ib-cr genes in extended-spectrum beta-lactamase (ESBL)-producing K. pneumonia strains isolated from burn wounds of patients in the city of Kermanshah, Iran. Methods: This descriptive-analytical study was conducted on 126 K. pneumonia strains isolated collected from burn wounds. Biochemical tests were used to detect the strains. The frequency of the ESBL-producing isolates was determined by phenotypic tests of the combination disk (CD) method after determining the antibiotic susceptibility pattern of the isolates through the Kirby-Bauer disc diffusion test. The prevalence of the qnr and aac(6’)-Ib-cr genes was determined using their special primers as well as polymerase chain reaction (PCR). Results: Of the 126 K. pneumonia isolates, 52 (41.3%) were identified as ESBL-producing strains. ESBL-producing isolates showed higher resistance against antibiotics than non-ESBL-producing ones. PMQR relevance and resistance to ciprofloxacin were, respectively, determined at 80.76% and 59.6%. The most frequent gene was aac(6’)-Ib-cr (n = 70, 55.6%), followed by the qnrB (n = 44, 34.9%. Conclusions: This study showed a high prevalence of qnr genes in ESBL-producing K. pneumonia isolates and antibiotic resistance. Given the horizontal transmission of antibiotic resistance genes among bacteria by mobile genetic elements, timely identification of infections caused by ESBL-producing and antimicrobial-resistant K. pneumonia strains is of paramount importance.

show abstract

“…[31][32][33] In the present study, the prevalence of oqxAB gene (6.3%) was very low as compared with previous reports. 23,29,31 Thus, indicating that it may not be a major mediator of fluoroquinolone resistance. qepA gene was not detected in the present study and similar findings has been documented from Thailand and Iran.…”

Section: Discussionmentioning

confidence: 99%

Fluoroquinolone Resistance in Clinical Isolates of Klebsiella Pneumoniae

et al. 2020

View full text Add to dashboard Cite

Introduction Fluoroquinolones are widely used broad-spectrum antibiotics. Recently, increased rate of resistance to this antibiotic has been observed in Klebsiella pneumoniae. The aim of the present study was to determine the presence of quinolone resistance determining regions (QRDR) mutation genes and plasmid-mediated quinolone resistance (PMQR) determinants in clinical isolates of ciprofloxacin-resistant K. pneumoniae. Material and Methods The study included 110 nonduplicate ciprofloxacin-resistant K. pneumoniae clinical isolates. Antibiotic susceptibility testing by disk diffusion method and minimum inhibitory concentration (MIC) by agar dilution methods for ciprofloxacin was performed according to the recommendations of Clinical Laboratory Standards Institute. The presence of QRDR genes and PMQR genes was screened by polymerase chain reaction (PCR) amplification. Result All 110 isolates were resistance to ciprofloxacin, levofloxacin, and ofloxacin. As much as 88% of the isolates exhibited high-level of MIC to ciprofloxacin. Among the 110 isolates, 94(85%) harbored gyrA and 85 (77%) gyrB. The parC and parE genes were detected in 88 (80%) and 64 (58%) isolates. qnrB was detected in 13 (12%) isolates and qnrS in 5 (4.5%) isolates. Two (1.8%) isolates carried both qnrB and qnrS genes. The acc (6')-Ib-cr gene was found in 98 (89%) isolates and oqxAB was detected in 7 (6.3%) isolates. One (0.9%) isolate carried qnrB, acc(6')-Ib-cr and oqxAB genes. Conclusion The prevalence of acc (6')-Ib-cr gene is high among PMQR determinants, followed by qnrB, oqxAB and qnrS.

show abstract

Prevalence of Plasmid-Mediated Quinolone Resistance Determinants and OqxAB Efflux Pumps among Extended-Spectrumβ-Lactamase ProducingKlebsiella pneumoniaeIsolated from Patients with Nosocomial Urinary Tract Infection in Tehran, Iran

Cited by 30 publications

References 38 publications

Bacterial plasmid-mediated quinolone resistance genes in aquatic environments in China

Bacterial plasmid-mediated quinolone resistance genes in aquatic environments in China

Frequency of qnr and aac(6’)Ib-cr Genes Among ESBL-Producing Klebsiella pneumoniae Strains Isolated from Burn Patients in Kermanshah, Iran

Fluoroquinolone Resistance in Clinical Isolates of Klebsiella Pneumoniae

Contact Info

Product

Resources

About