Fluoroquinolone Resistance in Clinical Isolates of Klebsiella Pneumoniae

Geetha, Pacha Venkataramana; Aishwarya, Kayanam Vijaya Lalitha; Shanthi, M; Sekar, Uma

doi:10.1055/s-0040-1716478

Cited by 21 publications

(21 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Only one strain (18k) was sensitive to ciprofloxacin and moxifloxacin, which corresponded to the lack of oqxA/qnrB genes in the bacterial genome. This high resistance to this class of antibiotics has been reported by other researchers in both Mexico and worldwide (Rodríguez-Zulueta et al, 2013;Osei Sekyere and Amoako, 2017;Salah et al, 2019;Geetha et al, 2020). Sulfonamide resistance genes such as sul1 or sul2 were highly prevalent (68%) in the K. pneumoniae strains analyzed in this study, which is in agree with other reports (Khamesipour and Tajbakhsh, 2016;Taitt et al, 2017).…”

Section: Discussionsupporting

confidence: 92%

Whole Genome Sequencing of Pediatric Klebsiella pneumoniae Strains Reveals Important Insights Into Their Virulence-Associated Traits

et al. 2021

View full text Add to dashboard Cite

Klebsiella pneumoniae is recognized as a common cause of nosocomial infections and outbreaks causing pneumonia, septicemia, and urinary tract infections. This opportunistic bacterium shows an increasing acquisition of antibiotic-resistance genes, which complicates treatment of infections. Hence, fast reliable strain typing methods are paramount for the study of this opportunistic pathogen’s multi-drug resistance genetic profiles. In this study, thirty-eight strains of K. pneumoniae isolated from the blood of pediatric patients were characterized by whole-genome sequencing and genomic clustering methods. Genes encoding β-lactamase were found in all the bacterial isolates, among which the blaSHV variant was the most prevalent (53%). Moreover, genes encoding virulence factors such as fimbriae, capsule, outer membrane proteins, T4SS and siderophores were investigated. Additionally, a multi-locus sequence typing (MLST) analysis revealed 24 distinct sequence types identified within the isolates, among which the most frequently represented were ST76 (16%) and ST70 (11%). Based on LPS structure, serotypes O1 and O3 were the most prevalent, accounting for approximately 63% of all infections. The virulence capsular types K10, K136, and K2 were present in 16, 13, and 8% of the isolates, respectively. Phylogenomic analysis based on virtual genome fingerprints correlated with the MLST data. The phylogenomic reconstruction also denoted association between strains with a higher abundance of virulence genes and virulent serotypes compared to strains that do not possess these traits. This study highlights the value of whole-genomic sequencing in the surveillance of virulence attributes among clinical K. pneumoniae strains.

show abstract

Section: Discussionsupporting

confidence: 92%

Whole Genome Sequencing of Pediatric Klebsiella pneumoniae Strains Reveals Important Insights Into Their Virulence-Associated Traits

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Klebsiella pneumonia is a crucial type of bacteria in the genus Klebsiella and accounts for more than 95% of Klebsiella infections ( Raetz et al, 1991 ; Geetha et al, 2020 ; Ota et al, 2020 ). Kp infection is acknowledged to be severe and challenging to treat, causing a high mortality rate.…”

Section: Discussionmentioning

confidence: 99%

Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-κB Pathways Against Klebsiella pneumonia Infection

et al. 2021

View full text Add to dashboard Cite

Background:Klebsiella pneumonia has emerged as a critical pathogen causing severe clinical problems, such as pneumonia and sepsis. Meanwhile, intensified drug resistance induced by antibiotic therapy necessitates discovering novel and active molecules from Traditional Chinese Medicine (TCM) for treatment.Methods and results: In this study, the isolated Bergenin monohydrate showed an anti-inflammatory effect in Klebsiella-infected mice. We initially investigated the anti-inflammatory effects and cytoprotection against oxidative stress in vitro and in vivo. Interestingly, a specific dose of Bm can effectively ameliorate lung injury and suppress the expression of inflammatory cytokines such as TNF-α, IL-6, IL-1β and PEG2. Moreover, Bm was also shown to reduced the levels of MPO, MDA and increased SOD and GSH activities. Moreover, we assessed the intracellular signaling molecules including p38, ERK, JNK, IκB, NF-κB-p65 by western blotting and verified through MAPK and NF-κB pathways inhibition experiments. These results reveal that Bm executed its effects via the classical MAPK signaling pathway and NF-κB pathway.Conclusion: Given its underlying anti-inflammatory effect, Bm may be used as a promising therapeutic against Klebsiella-induced infection, thus providing a benefit for the future clinical therapy of pneumonia and medicine design.

show abstract

“…After more than two decades of the discovery of plasmid-mediated resistance to quinolones (PMQR), the genes of resistance to quinolones that can be mobilized have been described mainly in Gram-negative bacteria of clinical importance, such as E. coli , P . vulgaris , Shigella , Salmonella , K. pneumoniae , Enterobacter , A. baumannii , and P. aeruginosa [ 210 , 211 , 212 , 213 , 214 , 215 , 216 , 217 , 218 ]. To date, three resistance mechanisms have been identified in transferable elements [ 77 , 219 ].…”

Section: Mechanisms Of Resistance To Quinolonesmentioning

confidence: 99%

“…The first mechanism is mediated by Qnr proteins that protect gyrase or topoisomerase IV from inhibition by quinolones. These proteins are encoded by the qnr genes of several families with multiple alleles, including the qnrA , qnrB , qnrC , qnrD , qnrE , qnrS , and qnrVC genes [ 77 , 210 , 211 , 212 , 213 , 214 , 215 , 216 , 217 , 218 , 220 ]. Qnr proteins are members of the PRP family (from pentapeptide repeat protein) that contain domains composed of repeating pentapeptides sequences of approximately 200 amino acids and it has been proposed that they act as DNA mimetics competing with DNA for its binding to type IV gyrase or topoisomerase [ 221 ].…”

Section: Mechanisms Of Resistance To Quinolonesmentioning

confidence: 99%

“…The second mechanism confers resistance by enzymatic modification of a variant of an aminoglycoside transferase that acetylates the unsubstituted nitrogen of the piperazine ring that has ciprofloxacin and norfloxacin in its structure, thus reducing the activity of these antimicrobials, as well as, that of some aminoglycosides and is encoded by the aac(6′)-Ib-cr gene [ 77 , 226 ]. It is a narrow spectrum mechanism inactivating only two FQs, however, it is widely distributed in Enterobacteriaceae [ 210 , 211 , 212 , 213 , 214 , 215 , 216 , 217 , 218 , 219 ]. Recently, in P. aeruginosa an ATP-phosphotransferase encoded by the crpP gene that inactivates ciprofloxacin has been described, finding homologous genes in clinical strains of extended-spectrum β-lactamase (ESBL)-producing enterobacteria, suggesting that this new gene may be highly distributed in Enterobacteriaceae [ 227 ].…”

Section: Mechanisms Of Resistance To Quinolonesmentioning

confidence: 99%

See 1 more Smart Citation

Biological Effects of Quinolones: A Family of Broad-Spectrum Antimicrobial Agents

et al. 2021

View full text Add to dashboard Cite

Broad antibacterial spectrum, high oral bioavailability and excellent tissue penetration combined with safety and few, yet rare, unwanted effects, have made the quinolones class of antimicrobials one of the most used in inpatients and outpatients. Initially discovered during the search for improved chloroquine-derivative molecules with increased anti-malarial activity, today the quinolones, intended as antimicrobials, comprehend four generations that progressively have been extending antimicrobial spectrum and clinical use. The quinolone class of antimicrobials exerts its antimicrobial actions through inhibiting DNA gyrase and Topoisomerase IV that in turn inhibits synthesis of DNA and RNA. Good distribution through different tissues and organs to treat Gram-positive and Gram-negative bacteria have made quinolones a good choice to treat disease in both humans and animals. The extensive use of quinolones, in both human health and in the veterinary field, has induced a rise of resistance and menace with leaving the quinolones family ineffective to treat infections. This review revises the evolution of quinolones structures, biological activity, and the clinical importance of this evolving family. Next, updated information regarding the mechanism of antimicrobial activity is revised. The veterinary use of quinolones in animal productions is also considered for its environmental role in spreading resistance. Finally, considerations for the use of quinolones in human and veterinary medicine are discussed.

show abstract

Fluoroquinolone Resistance in Clinical Isolates of Klebsiella Pneumoniae

Cited by 21 publications

References 35 publications

Whole Genome Sequencing of Pediatric Klebsiella pneumoniae Strains Reveals Important Insights Into Their Virulence-Associated Traits

Whole Genome Sequencing of Pediatric Klebsiella pneumoniae Strains Reveals Important Insights Into Their Virulence-Associated Traits

Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-κB Pathways Against Klebsiella pneumonia Infection

Biological Effects of Quinolones: A Family of Broad-Spectrum Antimicrobial Agents

Contact Info

Product

Resources

About