Prevalence of plasmid-mediated AmpC β-lactamases among Enterobacteriaceae in Algiers hospitals

Iabadene, Hassen; Messai, Yamina; Ammari, Houria; Alouache, Souhila; Verdet, Charlotte; Bakour, Rabah; Arlet, Guillaume

doi:10.1016/j.ijantimicag.2009.05.011

Cited by 39 publications

(29 citation statements)

References 10 publications

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“…Nucleotide sequencing of a representative group of these isolates (n = 7) revealed the bla CMY-2 gene. This observation is consistent with many other studies worldwide in which CMY-2 is the most commonly acquired plasmid and widely geographically distributed AmpC enzyme [5,[15][16][17][18][19][20][21][22].…”

Section: Discussionsupporting

confidence: 92%

Detection and epidemiology of plasmid-mediated AmpC β-lactamase producing Escherichia coli in two Irish tertiary care hospitals

Cassidy²,

Salmon

et al. 2015

Journal of Global Antimicrobial Resistance

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Section: Discussionsupporting

confidence: 92%

Detection and epidemiology of plasmid-mediated AmpC β-lactamase producing Escherichia coli in two Irish tertiary care hospitals

Cassidy²,

Salmon

et al. 2015

Journal of Global Antimicrobial Resistance

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“…9 In Algeria, only few reports on plasmid-encoded AmpC (CMY-2 and DHA-1) in Enterobacteriaceae strains recovered from hospital settings were published. [10][11][12] The aim of this study was to investigate the prevalence and molecular epidemiology of cefoxitin resistance among Enterobacteriaceae isolates recovered from hospitalized and non-hospitalized patients in Bejaia locality (Algeria). The association of pAmpC with extended-spectrum ␤-lactamase (ESBL) and plasmid-mediated quinolone resistance determinant was also studied.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and epidemiology of cefoxitin resistance among Enterobacteriaceae lacking inducible chromosomal ampC genes from hospitalized and non-hospitalized patients in Algeria: description of new sequence type in Klebsiella pneumoniae isolates

Gharout-Sait

Touati

Guillard

et al. 2015

The Brazilian Journal of Infectious Diseases

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In this study, 922 consecutive non-duplicate clinical isolates of Enterobacteriaceae obtained from hospitalized and non-hospitalized patients at Bejaia, Algeria were analyzed for AmpC-type β-lactamases production. The ampC genes and their genetic environment were characterized using polymerase chain reaction (PCR) and sequencing. Plasmid incompatibility groups were determined by using PCR-based replicon typing. Phylogenetic grouping and multilocus sequence typing were determined for molecular typing of the plasmid-mediated AmpC (pAmpC) isolates. Of the isolates, 15 (1.6%) were identified as AmpC producers including 14 CMY-4-producing isolates and one DHA-1-producing Klebsiella pneumoniae. All AmpC-producing isolates co-expressed the broad-spectrum TEM-1 β-lactamase and three of them co-produced CTX-M and/or SHV-12 ESBL. Phylogenetic grouping and virulence genotyping of the E. coli isolates revealed that most of them belonged to groups D and B1. Multilocus sequence typing analysis of K. pneumoniae isolates identified four different sequence types (STs) with two new sequences: ST1617 and ST1618. Plasmid replicon typing indicates that blaCMY-4 gene was located on broad host range A/C plasmid, while LVPK replicon was associated with blaDHA-1. All isolates carrying blaCMY-4 displayed the transposon-like structures ISEcp1/ΔISEcp1-blaCMY-blc-sugE. Our study showed that CMY-4 was the main pAmpC in the Enterobacteriaceae isolates in Algeria.

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“…There are few studies concerning the cephalosporinases AmpC diffusion in this species in Algeria. Only one study was conducted in three hospitals of Algiers, where there is the detection of the production of cephalosporinases CMY-2 in K. pneumoniae and DHA-1 was detected in Enterobacter cloacae strains (Iabadene et al, 2009). So, this is the first detection of DHA-1 in K. pneumoniae in Algeria.…”

Section: Discussionmentioning

confidence: 99%

First characterization of CTX-M-15 and DHA-1 -lactamases among clinical isolates of Klebsiella pneumoniae in Laghouat Hospital, Algeria

Lagha¹,

Abdelouahid²,

Hassaïne³

et al. 2014

Afr. J. Microbiol. Res.

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Extended-spectrum β-lactamases (ESBL) are a problem of great concern owing to the potential transmission of resistance to others bacterial species. Our study aims to investigate the ESBL and plasmid-mediated β-lactamases produced by Klebsiella pneumoniae strains isolated from 2010 to 2012, among patients hospitalized in "Ahemida Ben Adjila" hospital, Laghouat, Algeria, and to seek a possible clonal dissemination. Antimicrobial susceptibility testing was performed by the agar diffusion method, the characterization of resistance genes to β-lactam antibiotics was performed by PCR amplification and gene sequencing, and molecular typing was performed by ERIC-PCR. In total, of 112 clinical strains of K. pneumoniae isolated, nine isolates produced an ESBL. Antibiotics susceptibility testing showed a complete resistance to the majority of third-generation cephalosporins and a very frequent resistance to aminoglycosides and fluoroquinolones resistance. PCR analysis and sequencing showed that all isolates produced ESBL CTX-M-15; three strains of them also produced the cephalosporinase DHA-1. Molecular typing showed that most strains were not related; only three strains that produced CTX-M-15 and DHA-1 had identical profiles suggesting a clonality link. This study revealed the dissemination of ESBL CTX-M-15 and plasmid-mediated AmpC cephalosporinase DHA-1 in K. pneumoniae. This is a first report of these enzymes at the Laghouat hospital.

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Prevalence of plasmid-mediated AmpC β-lactamases among Enterobacteriaceae in Algiers hospitals

Cited by 39 publications

References 10 publications

Detection and epidemiology of plasmid-mediated AmpC β-lactamase producing Escherichia coli in two Irish tertiary care hospitals

Detection and epidemiology of plasmid-mediated AmpC β-lactamase producing Escherichia coli in two Irish tertiary care hospitals

Molecular characterization and epidemiology of cefoxitin resistance among Enterobacteriaceae lacking inducible chromosomal ampC genes from hospitalized and non-hospitalized patients in Algeria: description of new sequence type in Klebsiella pneumoniae isolates

First characterization of CTX-M-15 and DHA-1 -lactamases among clinical isolates of Klebsiella pneumoniae in Laghouat Hospital, Algeria

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