Prevalence of pathogens causing acute diarrheal disease in the Asunción and Central Metropolitan area

Huber, Claudia; Orrego, Maria; Ortiz, Flavia; Álvarez, Mercedes; Weiler, Natalie

doi:10.18004/rspp.2019.diciembre.41-45

Cited by 4 publications

(4 citation statements)

References 5 publications

(6 reference statements)

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“…Our results confirm the possibility that mixed infections by two pathotypes of DEC can be detected in a sample, as mentioned in other studies (Huber et al, 2019;Odetoyin et al, 2016 ). This PCR was highly specific with the primers chosen for the detection of six categories of DEC.…”

Section: Discussionsupporting

confidence: 90%

“…Our study differ from other reports by Verma et al (2019), Thakur et al (2018) in India which suggested low prevalence of ETEC 6%, 5.7%. While in Huber et al (2019), ETEC was recognized ETEC as major pathotype among children less than 5 years, and in the study by Hasan et al (2020) in Dohuk /Iraq, the isolation rate of ETEC was the highest. Low-grade hygienic state of family and artificial feeding may account for this high rate of ETEC.…”

Section: Discussionmentioning

confidence: 87%

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Detection of Diarrheagenic E. Coli Among Children Under ‎‏5‏‎ Year’s Age in Tikrit City of Iraq by Using Single Multiplex PCR ‎technique

Shatub¹,

Jafar²,

Melconian³

2021

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A variety of diarrheagenic E coli (DEC) are responsible for causing diarrhea in children under five years age especially in developing courtiers. The aim of this study was to isolate and bacteriological characterizing of DEC from diarrheic children's stool and using molecular identification of DEC pathotypes for better discrimination and investigate their antibiotic resistance patterns. Total of 200 fresh stools specimens were collected from children with diarrhea in Salah Aldin Hospital in Tikrit city, Iraq. The samples were cultured on selective media such as (MacConkey and EMB). Colonies were identified through biochemical reaction and api 20E system and then antibiotic susceptibility profiles were determined. E. coli were isolated and characterized from 200 diarrheagenic stool positive samples, collected from hospitalized children less than 5 years old. A total of 75(40.5%) samples were yielded positive for growth of E. coli Out of these, 46 genotypically-identified DEC were then subjected to multiplex PCR assay targeting certain virulence factors ( SHIG, bfpA, eae, LT, ST, EA, vt1, vt2, daaE, uidA ) for discrimination of pathotyes. 21/46(45.7%) EPEC with 18 atypical and 3 typical, 11/46(24%) ETEC, 3/46 /(6.5%) EAEC, 2/46 (4.3%) for EIEC was detected, 2/46(4.3%) EHEC was also detected, while no DAEC was detected, Also mix pathotypes were detected, more than one pathotype was observed in a number of samples 7/46(15.2%), 5 (aEPEC + ETEC), 2 (aEPEC + EAEC). Moreover, all pathotypes expressed high resistant to Cefotaxime, ampicillin, Piperacillin, Azitromycin, Amikacin. while little resistance to Imipenem, Meropenem, Ciprofloxacin, Norfloxacin was observed. The study concludes EPEC is the dominant pathotype between DEC pathotypes in our society that causes diarrhea in children, and emphasizes the importance of using mPCR assay for best discrimination.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 87%

Detection of Diarrheagenic E. Coli Among Children Under ‎‏5‏‎ Year’s Age in Tikrit City of Iraq by Using Single Multiplex PCR ‎technique

Shatub¹,

Jafar²,

Melconian³

2021

PLANT ARCHIVES

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“…In Asunción and the metropolitan area, diarrheagenic Escherichia coli, Rotavirus, Campylobacter spp., Shigella spp., and Salmonella spp. have been described as the most prevalent etiological agents in children under 5 years of age (31).…”

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confidence: 99%

Epidemiological characteristics of mortality from infectious intestinal diseases (diarrhea) Paraguay (2015-2019)

Merles Aranda,

Tullo Gómez,

Duarte Zoilán

et al. 2024

Med Clín Soc

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Introduction: Infectious intestinal diseases (diarrhea) are a major cause of morbidity and mortality worldwide. In 2015, it constituted the ninth leading cause of death for all ages. Objective: To describe the epidemiological characteristics of mortality due to infectious intestinal diseases (diarrhea) in Paraguay from 2015 to 2019. Methods: Descriptive, ecological, cross-sectional, retrospective, and non-probabilistic sampling of consecutive cases was performed. ICD-10 deaths included cholera (A01), typhoid and paratyphoid fever (A01), shigellosis (A03), other bacterial intestinal infections (A04), other bacterial food poisoning (A05), amebiasis (A06), other intestinal diseases due to protozoa (A07), intestinal infections due to viruses (A08), and diarrhea and gastroenteritis of presumed infectious origin (A09). Open access data were obtained from the WEB page of the General Directorate of Strategic Information in Health of the MSP and BS of all the Departments of the country. Crude and adjusted rates (per 100,000 inhabitants) were calculated. Excel and EPI INFO 7.0 were used. Results: 495 deaths were registered; the highest prevalence was in 2019 (adjusted rate of 1.83) and the lowest in 2017 (1.36). The months with the highest mortality rates were January and July (9.9%). 51.72% were women (256), 30.91% were over 80 years old and 28.28% (140) were under 5 years old, average age 50.8, single 56.77% (281) and without any type of education 41.82% (207). A total of 80.61% (399) of the respondents resided in urban areas. The highest rates were registered in Boquerón (33.3) and Amambay (15.2). Diarrhea and gastroenteritis of infectious origin accounted for 95.56% of the cases (473). Discussion: There was a high percentage of mortality from infectious diarrhea. Extremes of life were the most vulnerable populations.

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“…Sur para el 2015 es de 23,6 kg/persona/año, demostrando un incremento progresivo del 66% con relación a años anteriores, siendo Brasil el país con mayor consumo per ISSN: 2600-5859 Otro de los agentes patológicos predominantes en el estudio es la bacteria E. coli causante de enfermedades como: síndrome diarreico, colitis hemorrágica, síndrome urémico hemolítico y púrpura trombocitopénica trombótica, infecciones del tracto urinario y otros padecimientos infecciosos, por consumo de productos lácteos, vegetales, frutas, productos cárnicos y alimentos preparados en restaurantes y los síntomas y signos son diarrea con o sin sangre, fiebre, vómito, dolor y calambres abdominales. Este dato coincide con lo reportado porHuber et al (2019), quienes manifiestan que el síndrome diarreico es una de las enfermedades más prevalentes que origina la E. coli. De igual manera,Alarcon et al (2019) revelan que esta bacteria puede generar infecciones urinarias.…”

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Enfermedades transmitidas por bacterias patógenas presentes en los alimentos en América del Sur, artículo de revisión

Delgado Chiriboga,

Monge Jachero,

Verdugo García

2023

ConcienciaDigital

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Resumen Introducción. Las Enfermedades transmitidas por bacterias patógenas constituyen un tema de salud pública a nivel mundial, estas afectan especialmente a los países de ingresos bajos y medios, por ejemplo, los países de América del Sur. Objetivo. Describir las principales enfermedades transmitidas por bacterias patógenas presentes en los alimentos en América del Sur. Metodología. Este artículo es de revisión bibliográfica; para la selección de los artículos científicos se utilizó las bases de datos electrónicas desde el año 2016 debido a la falta de artículos científicos recientes. Además, se utilizó el Método PRISMA para la clasificación de la información donde se incluyeron 43 estudios. Resultados. Las enfermedades trasmitidas por bacterias de mayor predominancia fue la salmonelosis (17/38; 44,7%). Los agentes etiológicos frecuentes fueron la Salmonella spp. en un 46,5% y E. coli en un 25,6%. En Ecuador, Colombia, Perú y Chile, la enfermedad transmitida por bacterias que tuvo mayor predominio fue la salmonelosis en un 41,7%, 24,5%, 30% y 50%, respectivamente; en Paraguay y Brasil, el síndrome diarreico y en Argentina la colitis hemorrágica, síndrome urémico hemolítico y púrpura trombocitopénica trombótica. Conclusión. La enfermedad transmitida por bacterias de mayor predominancia en América del Sur es la salmonelosis, los agentes etiológicos más frecuentes asociados a brotes fueron Salmonella spp. y E. coli. La salmonelosis predomina en Ecuador, Colombia, Perú y Chile, mientras que en Paraguay y Brasil fue el síndrome diarreico y en Argentina la colitis hemorrágica, síndrome urémico hemolítico y púrpura trombocitopénica trombótica. Área de estudio general: medicina. Área de estudio específica: tropicales y desatendidas. Tipo de estudio: revisión bibliográfica.

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Prevalence of pathogens causing acute diarrheal disease in the Asunción and Central Metropolitan area

Cited by 4 publications

References 5 publications

Detection of Diarrheagenic E. Coli Among Children Under ‎‏5‏‎ Year’s Age in Tikrit City of Iraq by Using Single Multiplex PCR ‎technique

Detection of Diarrheagenic E. Coli Among Children Under ‎‏5‏‎ Year’s Age in Tikrit City of Iraq by Using Single Multiplex PCR ‎technique

Epidemiological characteristics of mortality from infectious intestinal diseases (diarrhea) Paraguay (2015-2019)

Enfermedades transmitidas por bacterias patógenas presentes en los alimentos en América del Sur, artículo de revisión

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