Prevalence of Multidrug-resistant Pseudomonas aeruginosa Strains in Ardabil

Bazghandi, Seyed Ali; Safarirad, Somayeh; Arzanlou, Mohsen; Peeridogaheh, Hadi; Alimohammadi, Hossein; Khademi, Farzad

doi:10.52547/jarums.20.2.280

Cited by 7 publications

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“…Bacterial isolates were collected from different specimens of patients who were referred to Ardabil hospitals from June 2019 to February 2021. The collected isolates were confirmed by standard laboratory tests, including pigment production, Gram-staining, catalase, and oxidase, as well as species-specific PCR (10). Confirmed bacteria were tested to identify imipenem resistance and MBL-positive isolates using phenotypic and genotypic methods.…”

Section: P Aeruginosa Isolatesmentioning

confidence: 99%

Prevalence and Characteristics of Metallo-beta-Lactamase-positive and High-risk Clone ST235 Pseudomonas aeruginosa at Ardabil Hospitals

Safarirad

Arzanlou

Mohammadshahi

et al. 2021

Jundishapur J Microbiol

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Background: Carbapenems are the most commonly administered drugs for the treatment of multidrug-resistant Pseudomonas aeruginosa (MDR-P. aeruginosa) infections. However, carbapenem-resistant P. aeruginosa is spreading rapidly and has led to a new threat to human health worldwide. Objectives: The current study aimed to determine the prevalence of imipenem-resistant P. aeruginosa, detect metallo-β-lactamase (MBL)-producer isolates, and evaluate their clonal relationships in strains isolated from patients referring to the hospitals of Ardabil city, Iran. Methods: The resistance rate to imipenem was evaluated using the disk diffusion method. Double-disk synergy test and PCR technique were used for phenotypic and genotypic screening of MBL-positive P. aeruginosa, respectively. Ultimately, ERIC-PCR and MLST methods were used for assessing clonal relatedness among the isolates. Results: The prevalence of imipenem-resistant P. aeruginosa strains was estimated at 57.1% (48 out of 84 isolates). In addition, 45 (93.7%) out of 48 imipenem-resistant P. aeruginosa isolates were phenotypically screened as MBL-positive, among which 16 (35.5%) and three (6.6%) isolates harbored blaIMP and blaVIM-1 genes, respectively. However, blaNDM, blaSIM-2, blaSPM, and blaGIM-1 genes were not detected in this study. MBL-producing P. aeruginosa strains were divided into 42 ERIC-PCR types. Based on the results of MLST, P. aeruginosa ST235 was the only identified sequence type. Conclusions: Our results revealed a high and alarming prevalence of imipenem-resistant and blaIMP-positive P. aeruginosa ST235 at Ardabil hospitals. Continuous monitoring is essential to control the further spread of this highly virulent and drug-resistant clone.

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Section: P Aeruginosa Isolatesmentioning

confidence: 99%

Prevalence and Characteristics of Metallo-beta-Lactamase-positive and High-risk Clone ST235 Pseudomonas aeruginosa at Ardabil Hospitals

Safarirad

Arzanlou

Mohammadshahi

et al. 2021

Jundishapur J Microbiol

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“…Our study's findings were similar to those of Farva and Bhutta (2021), who reported a prevalence of 30%, and Ijaz et al (2019), who reported a prevalence of 38%, but they were lower than those of Nasser et al (2018), who reported a prevalence of 46%. Several classes of antibiotics have been described as being utilized as antipseudomonas medications in various research (Nasser et al, 2018;Bazghandi et al, 2020;Abdulhaq et al, 2020). We used the following antibiotics in this study: imipenem (10 g), meropenem (10 g), cefepime (30 g), ciprofloxacin (5 g), amikacin (30 g), ceftriaxone (30 g), ceftazidime (30 g), and gentamycin (10 g).…”

Section: Synergistic Effect Of Agnps With Drugsmentioning

confidence: 99%

Synergistic Effect of Biogenic Silver Nanoparticles and Antibiotics Against Multidrug-Resistant Pseudomonas aeruginosa

Jalal

Hariri

Abdel-razik

et al. 2023

Egyptian Academic Journal of Biological Sciences. C, Physiology

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Infections caused by Pseudomonas aeruginosa may be either acquired in the community or contracted in a healthcare setting. Multidrug-resistant (MDR) P. aeruginosa is a growing problem; a new treatment approach is required to tackle this. Combination therapy of antibiotics and nanoparticle is thus applied to overcome this problem. Therefore, this study was planned to evaluate the synergistic effect of AgNPs along with different antibiotics against MDR P. aeruginosa. A total of 120 surgical or burn wound samples were collected from a tertiary care hospital. The plates containing the samples cultivated on cetrimide agar were then heated to 37°C. Isolates were identified based on colony shape, Gram staining, and several biochemical tests. A Kirby-Bauer disc diffusion technique antibiogram was conducted following CLSI 2022 recommendations. A minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also determined. The Agar well diffusion technique and the microdilution method were used to test the antibacterial activity of the AgNPs, respectively. The synergistic effect of antibiotics and AgNPs was estimated by the Checkerboard method. Out of 120 samples, 46 (38.8%) were confirmed positive for P. aeruginosa, and out of that, 33 were confirmed as MDR P. aeruginosa. Seven representative isolates proceeded for further procedures. Antibacterial activity of AgNPs revealed a maximum zone of inhibition of 12 mm at 4 mg/ml and a minimum of 2.5 mm at 1 mg/ml by agar well diffusion method. MIC and MBC of AgNPs showed that all the isolates were inhibited at 250 mg/ml. The FIC index of checkerboard results showed that colistin and gentamicin exhibited complete synergism with AgNPs, while ciprofloxacin showed partial synergism with AgNPs.

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Prevalence of resistance genes to biocides in antibiotic-resistant Pseudomonas aeruginosa clinical isolates

et al. 2021

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Background Biocides are frequently used as preservative, disinfectant and sterilizer against many microorganisms in hospitals, industry and home. However, the reduced susceptibility rate of Pseudomonas aeruginosa (P. aeruginosa) strains to biocides is increasing. The aim of this study was to evaluate the antimicrobial activity of four frequently used biocides against P. aeruginosa and to determine the prevalence of genes involved in biocide resistance. Methods A total of 76 clinical isolates of P. aeruginosa strains were used in the present study. The minimum inhibitory concentrations (MICs) of four biocides, i.e. chlorhexidine digluconate, benzalkonium chloride, triclosan and formaldehyde, against P. aeruginosa strains were determined using agar dilution method. In addition, the prevalence of biocide resistance genes was determined using the polymerase chain reaction (PCR) method. ResultsIn the present study, the highest MIC 90 and MIC 95 (epidemiological cut-off) values were observed for benzalkonium chloride (1024 μg/mL), followed by formaldehyde (512 μg/mL), triclosan (512 μg/mL) and chlorhexidine digluconate (64 μg/ mL). Furthermore, the prevalence of qacEΔ1, qacE, qacG, fabV, cepA and fabI genes were 73.7% (n = 56), 26.3% (n = 20), 11.8% (n = 9), 84.2% (n = 64), 81.5% (n = 62) and 0% (n = 0), respectively. A significant association was observed between the presence of biocide resistance genes and MICs (p < 0.05). Furthermore, there was no significant association between the presence of biocide resistance genes and antibiotic resistance (p > 0.05), except for levofloxacin and norfloxacin antibiotics and qacE and qacG genes (p < 0.05). Conclusion Our results revealed that chlorhexidine digluconate is the most effective biocide against P. aeruginosa isolates in Ardabil hospitals. However, we recommend continuous monitoring of the antimicrobial activity of biocides and the prevalence of biocide-associated resistance genes for a better prevention of microorganism dissemination and infection control in hospitals.

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Prevalence of Multidrug-resistant Pseudomonas aeruginosa Strains in Ardabil

Cited by 7 publications

References 0 publications

Prevalence and Characteristics of Metallo-beta-Lactamase-positive and High-risk Clone ST235 Pseudomonas aeruginosa at Ardabil Hospitals

Prevalence and Characteristics of Metallo-beta-Lactamase-positive and High-risk Clone ST235 Pseudomonas aeruginosa at Ardabil Hospitals

Synergistic Effect of Biogenic Silver Nanoparticles and Antibiotics Against Multidrug-Resistant Pseudomonas aeruginosa

Prevalence of resistance genes to biocides in antibiotic-resistant Pseudomonas aeruginosa clinical isolates

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