Prevalence of metallo-β-lactamase-encoding genes among carbapenem-resistant Pseudomonas aeruginosa strains isolated from burn patients in Iran

Jabalameli, Fereshteh; Taki, Elahe; Emaneini, Mohammad; Beigverdi, Reza

doi:10.1590/0037-8682-0044-2018

Cited by 30 publications

(16 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similarly, a study from Japan which included 180 P. aeruginosa reported that bla IMP-1 was the predominant gene detected (116/180 isolates) followed by bla VIM-2 (63/180 isolates) [16]. While a meta-analysis from Iran which included P. aeruginosa from burn patients reported that the pooled prevalence of bla VIM was 21.4% and of bla IMP was 13.1%, the reported rates were higher than in this study [30]. A study from Iran also reported that bla VIM (17.5%) was the most common gene followed by bla IMP (15.6%) [15].…”

Section: Discussioncontrasting

confidence: 74%

“…A much higher rate was reported from Russia (75.3%) and Costa Rica (63.1%) [9]. A meta-analysis from Iran, which included 14 publications on P. aeruginosa isolated from burn patients, reported a pooled prevalence rate of 76.8% carbapenemresistant P. aeruginosa, which is much higher than that reported in this study [30]. However, a study from China, which included P. aeruginosa isolated from cystic fibrosis patients, reported that 56.25% of their isolates were found to be MBL producers, which is comparable to that reported in this study [21].…”

Section: Discussioncontrasting

confidence: 71%

See 1 more Smart Citation

Prevalence of metallo-β-lactamase genes among Pseudomonas aeruginosa isolated from various clinical samples in China

Wang

2020

Journal of Laboratory Medicine

View full text Add to dashboard Cite

BackgroundPseudomonas aeruginosa is an opportunistic pathogen which is associated with nosocomial infections and causes various diseases including urinary tract infection, pneumonia, soft-tissue infection and sepsis. The emergence of P. aeruginosa-acquired metallo-β-lactamase (MBL) is most worrisome and poses a serious threat during treatment and infection control. The objective of this study was to identify antibiotic susceptibility, phenotypic detection of MBL production and to determine the prevalence of MBL genes in carbapenem-resistant P. aeruginosa isolated from different clinical samples.MethodsA total of 329 non-duplicate P. aeruginosa isolated from various clinical samples from two hospitals in China between September 2017 and March 2019 were included in this study. Phenotypic detection of MBL was performed by the combined detection method using imipenem and imipenem-ethylenediaminetetraacetic acid (EDTA) discs. MBL-encoding genes including blaVIM-1, blaVIM-2, blaIMP-1, blaIMP-2, blaSPM-1, blaSIM, blaNDM-1 and blaGIM were detected by polymerase chain reaction (PCR).ResultsOf the 329 P. aeruginosa, majority of the isolates were resistant to imipenem (77.5%) followed by meropenem (64.7%). Of the 270 P. aeruginosa isolates tested, 149 (55.2%) isolates were found to be positive for MBL detection. Of the different samples, 57.8% (n = 26) of P. aeruginosa isolated from blood were found to be positive for MBL production. Of the various MBL genes, blaIMP-1 (28.2%) was the most predominant gene detected followed by blaVIM-2 (18.8%), blaVIM-1 (16.1%), blaNDM-1 (9.4%), blaIMP-2 (6.7%), blaSIM (6.0%), blaSPM-1 (4.0%) and blaGIM (1.3%) genes.ConclusionsThe high resistance of P. aeruginosa toward imipenem and meropenem and the high prevalence of blaIMP-1 and blaVIM-2 set the alarm on the increasing, perhaps the increased, carbapenem resistance. In addition to routine antibiotic susceptibility testings, our results emphasize the importance of both the phenotypic and genotypic MBL detection methods in routine practice for early detection of carbapenem resistance and to prevent further dissemination of this resistant pathogen.

show abstract

Section: Discussioncontrasting

confidence: 74%

Section: Discussioncontrasting

confidence: 71%

Prevalence of metallo-β-lactamase genes among Pseudomonas aeruginosa isolated from various clinical samples in China

Wang

2020

Journal of Laboratory Medicine

View full text Add to dashboard Cite

show abstract

“…13 In the present study, there were five IMP-positive strains (15.5%) and four VIM-positive strains (12.5%), similar to the results reported previously. 14,15 However, 27 strains were SIM-positive (84.4%), which was the most prevalent MBL gene in our study. This result is inconsistent with previous studies, maybe due to the difference in antibiotic usage in different areas.…”

Section: Discussionmentioning

confidence: 48%

<p>High Expression of Metallo-β-Lactamase Contributed to the Resistance to Carbapenem in Clinical Isolates of <em>Pseudomonas aeruginosa</em> from Baotou, China</p>

Xu¹,

Niu²,

TongPing³

et al. 2020

IDR

View full text Add to dashboard Cite

Background: Bacterial resistance to antibiotics has become a major public health concern. This study aimed to determine the resistance mechanisms to carbapenem in clinical isolates of Pseudomonas aeruginosa. Methods: A total of 62 clinical isolates of carbapenem-resistant P. aeruginosa (CRPA) were collected from 2015 to 2017. Imipenem (IPM)-EDTA disk synergy test was used to screen strains that produced metallo-β-lactamase. In addition, the genes for outer membrane protein OprD2, metallo-β-lactamase and mexR gene were amplified and sequenced. Expression of mexA was detected by real-time PCR. Results: Disk synergy test showed that 51.6% (32/62) of the strains were positive for metallo-βlactamase. PCR showed that 84.4% of the strains were SIM-positive (27/32), 15.6% of the strains were IMP-positive (5/32), and 12.5% of the strains were VIM-positive (4/32). SPM-positive and GIM-positive strains were not detected. In addition, 5 of the 62 strains had small deletions and/or point mutations in OprD2. Three strains had a high expression of mexA, while eight strains were positive for the regulatory gene mexR with no mutations detected by DNA sequencing. Conclusion: Expression of metallo-β-lactamase is the main resistance mechanism of P. aeruginosa to carbapenem. Mutations in OprD2 and/or the overexpression of efflux pump MexAB-OprM may contribute to P. aeruginosa resistance to carbapenem.

show abstract

“…In recent years carbapenem resistance in A. baumannii isolates are increasing in the world. Various factors have been mentioned to contribute to this outbreak, for example, inadequate implementation of treatment instructions and protocols, excessive use of antimicrobial agents in health care systems or the community [139,140] . It is suggested that controlling policy including standard administration guidelines together with the suitable drug, dosage as well as the duration of treatment should meticulously be monitored in order to prevent the emergence of resistance among bacteria.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Imipenem-Resistant Acinetobacter baumannii isolates in Iran: A Meta-Analysis

Derakhshan

Heidary

Shams

2021

IEM

View full text Add to dashboard Cite

Background: Acinetobacter baumannii is a gram-negative pathogen that is highly resistant to antibiotics. This bacterium can cause severe systemic infections, especially in hospitalized patients. Recently, antimicrobial-resistant Acinetobacter baumannii has become a lifethreatening pathogen in Iran and around the world. Materials & Methods: In this study, several Iranian and English databases were systematically searched to find all original and review articles investigating the prevalence of imipenem resistance in their sample size, while mentioning the source of clinical isolates, as well as the prevalence of antimicrobial resistance genes. Findings: Among genes, bla OXA-23 with a prevalence of 31% to 100% was responsible for global outbreaks of imipenem-resistant Acinetobacter baumannii and was presented in most of the hospital isolates. Our meta-analysis also revealed that 74.2% of Acinetobacter baumannii were resistant to imipenem in 122 clinical studies. Conclusion:Our study highlighted a rapid increase in the rate of imipenem resistance in clinical isolates of Acinetobacter baumannii in Iran. The need for periodic antibiotic care system programs to monitor the administration and use of antibiotics

show abstract

Prevalence of metallo-β-lactamase-encoding genes among carbapenem-resistant Pseudomonas aeruginosa strains isolated from burn patients in Iran

Cited by 30 publications

References 26 publications

Prevalence of metallo-β-lactamase genes among Pseudomonas aeruginosa isolated from various clinical samples in China

Prevalence of metallo-β-lactamase genes among Pseudomonas aeruginosa isolated from various clinical samples in China

<p>High Expression of Metallo-β-Lactamase Contributed to the Resistance to Carbapenem in Clinical Isolates of <em>Pseudomonas aeruginosa</em> from Baotou, China</p>

Prevalence of Imipenem-Resistant Acinetobacter baumannii isolates in Iran: A Meta-Analysis

Contact Info

Product

Resources

About