Prevalence of Lyme disease spirochetes in Ixodes persulcatus and wild rodents in far eastern Russia

Sato, Yukita; Miyamoto, Kenji; Iwaki, Atsue; Masuzawa, Toshiyuki; Yanagihara, Yasutake; Éi, Korenberg; Горелова, Н. Б.; Volkov, V I; Иванов, Л. И.; Liberova, R N

doi:10.1128/aem.62.10.3887-3889.1996

Cited by 29 publications

(17 citation statements)

References 13 publications

(13 reference statements)

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“…The observed distribution of genospecies is in agreement with the results of earlier studies performed in Finland and Russia (14,28). B. burgdorferi sensu stricto has been detected only in the southwestern parts of Finland, and the prevalence of B. garinii seems to increase toward the eastern border.…”

Section: Discussionsupporting

confidence: 91%

Prevalence of Borrelia burgdorferi in Ixodes ricinus Ticks in Urban Recreational Areas of Helsinki

Junttila

Peltomaa

Soini³

et al. 1999

J Clin Microbiol

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Lyme borreliosis, an infection caused by the tick-borne spirocheteBorrelia burgdorferi, is a major health problem for populations in areas of endemicity in the Northern Hemisphere. In the present study we assessed the density of ticks and the prevalence ofB. burgdorferi sensu lato among ticks in popular urban recreational areas of Helsinki, Finland. Altogether 1,688 Ixodes ricinus ticks were collected from five areas located within 5 km of the downtown section of Helsinki, and 726 of them (303 nymphs, 189 females, and 234 males) were randomly chosen for laboratory analysis. The midguts of the ticks were divided into three pieces, one for dark-field microscopy, one for cultivation in BSK-II medium, and one for PCR analysis. Ticks were found in all the study areas; their densities varied from 1 to 36 per 100 m along which a cloth was dragged. The rate of tick infection with B. burgdorferi sensu lato varied from 19 to 55%, with the average being 32%. Borellia afzelii was the most predominant genospecies in all the areas, and no B. burgdorferi sensu stricto isolates were detected. Only two ticks were concurrently infected with both B. afzelii and Borrelia garinii. Dark-field microscopy gave more positive results for B. burgdorferi than did cultivation or PCR analysis. However, the agreement between all three methods was fairly good. We conclude that Lyme borreliosis can be contracted even in urban environments not populated with large mammals like deer or elk. The disease should be taken into account in the differential diagnosis of certain symptoms of patients from these areas, and the use of measures to improve the awareness of the general population and health care officials of the risk of contracting the disease is warranted.

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Section: Discussionsupporting

confidence: 91%

Prevalence of Borrelia burgdorferi in Ixodes ricinus Ticks in Urban Recreational Areas of Helsinki

Junttila

Peltomaa

Soini³

et al. 1999

J Clin Microbiol

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“…Six B. burgdorferi sensu lato species have been reported in Far Eastern Russia and Asian countries. B. garinii and B. afzelii are largely found throughout the range of I. persulcatus ticks in China (129,248,290), Japan (72,164), Korea (181), and Far Eastern Russia (147,200,218). B. japonica, B. tanukii, and B. turdi are limited to Japan (68,116,149).…”

Section: Epidemiological Implications Geographic Distribution Of Diffmentioning

confidence: 99%

Molecular Typing ofBorrelia burgdorferiSensu Lato: Taxonomic, Epidemiological, and Clinical Implications

et al. 1999

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SUMMARY Borrelia burgdorferi sensu lato, the spirochete that causes human Lyme borreliosis (LB), is a genetically and phenotypically divergent species. In the past several years, various molecular approaches have been developed and used to determine the phenotypic and genetic heterogeneity within the LB-related spirochetes and their potential association with distinct clinical syndromes. These methods include serotyping, multilocus enzyme electrophoresis, DNA-DNA reassociation analysis, rRNA gene restriction analysis (ribotyping), pulsed-field gel electrophoresis, plasmid fingerprinting, randomly amplified polymorphic DNA fingerprinting analysis, species-specific PCR and PCR-based restriction fragment length polymorphism (RFLP) analysis, and sequence analysis of 16S rRNA and other conserved genes. On the basis of DNA-DNA reassociation analysis, 10 different Borrelia species have been described within the B. burgdorferi sensu lato complex: B. burgdorferi sensu stricto, Borrelia garinii, Borrelia afzelii, Borrelia japonica, Borrelia andersonii, Borrelia valaisiana, Borrelia lusitaniae, Borrelia tanukii, Borrelia turdi, and Borrelia bissettii sp. nov. To date, only B. burgdorferi sensu stricto, B. garinii, and B. afzelii are well known to be responsible for causing human disease. Different Borrelia species have been associated with distinct clinical manifestations of LB. In addition, Borrelia species are differentially distributed worldwide and may be maintained through different transmission cycles in nature. In this paper, the molecular methods used for typing of B. burgdorferi sensu lato are reviewed. The current taxonomic status of B. burgdorferi sensu lato and its epidemiological and clinical implications, especiallly correlation between the variable clinical presentations and the infecting Borrelia species, are discussed in detail.

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“…Only two major genospecies, B. garinii and B. afzelii, have been reported in this tick species, and B. garinii was predominant [8]. In addition, B. japonica, whose pathogenic role is still unknown, was identi¢ed in the Russian Far East [9].…”

Section: Introductionmentioning

confidence: 98%

Detection and typing ofBorrelia burgdorferisensu lato genospecies inIxodes persulcatusticks in West Siberia, Russia

Беклемишев¹,

Dobrotvorsky

Piterina³

et al. 2003

FEMS Microbiology Letters

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The prevalence of Borrelia burgdorferi sensu lato (s.l.) genospecies in West Siberia as well as in many other regions of Russia remains insufficiently investigated. In the present study a total of 151 adult female ticks Ixodes persulcatus Schulze, collected at three localities in eastern regions of West Siberia, where Lyme disease is endemic, were examined for the presence of the spirochete B. burgdorferi s.l. by polymerase chain reaction targeting the 23S^5S rRNA intergenic spacer regions. Spirochetal DNA was detected in on average 15.2 þ 3.0% of the ticks examined. The infection rate of adult ticks with B. burgdorferi s.l. at various localities ranged from 8.6 þ 3.4% to 29.0 þ 7.6%, being greatest in the northernmost site studied and decreasing southwards. The restriction patterns obtained after MseI digestion of the 23S^5S rRNA intergenic spacer amplicons assigned 23 DNA samples to the following genomic groups: 19 to B. garinii (12 to group NT29 and seven to group 20047 T ), three to B. afzelii, and one to mixed B. afzelii and B. garinii NT29. We have not detected other genospecies, which were found in ticks in Europe, the Russian Far East and Japan. Thus, the ticks examined were associated only with two genospecies of Borrelia burgdorferi s.l. pathogenic to humans (B. garinii and B. afzelii), and B. garinii was the major genospecies infecting adult I. persulcatus in eastern regions of West Siberia. ß

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Prevalence of Lyme disease spirochetes in Ixodes persulcatus and wild rodents in far eastern Russia

Cited by 29 publications

References 13 publications

Prevalence of Borrelia burgdorferi in Ixodes ricinus Ticks in Urban Recreational Areas of Helsinki

Prevalence of Borrelia burgdorferi in Ixodes ricinus Ticks in Urban Recreational Areas of Helsinki

Molecular Typing ofBorrelia burgdorferiSensu Lato: Taxonomic, Epidemiological, and Clinical Implications

Detection and typing ofBorrelia burgdorferisensu lato genospecies inIxodes persulcatusticks in West Siberia, Russia

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