Prevalence of Listeria spp. in fish obtained from Urmia fish markets

Abstract

The present study was conducted to determine the prevalence of Listeria spp. in fish obtained from Urmia fish markets. A number of 194 fish comprising Oncorhynchus mykiss (n=42), Sander lucioperca (n=38), Cyprinus carpio (n=30), Hypophthalmiachthys molitrix (n=48), Abramis brama (n=12), Astacus leptodactylus (n=12) and Silurus glanis (n=12) were obtained from different fish markets of Urmia from June 2009 to February 2010. Listeria isolation was performed in two stages including enrichment in cold and selective plating. After colony formation, in order to confirming the genus of the Listeria, a fragment of Prs gene using Listeria genus specific primers was amplified from isolated bacteria using polymerase chain reaction (PCR). Results revealed that Listeria was isolated from 24 fish (12.37%). The highest prevalence of Listeria was observed in both A. brama and A. leptodactylus with 25%, while the lowest prevalence of Listeria was seen in S. lucioperca (9.7%). From the total of 24 Listeria isolates, five isolates (21%) were confirmed to be L. monocytogenes; seven isolates were L. ivonoi (29%) while L. Seeligeri was not isolated from any examined fish. The study showed that L. monocytogenes and other Listeria species are common contaminant of fish obtained from Urmia fish markets, and this may pose serious public health implications.

Prevalence and molecular characterization of Listeria spp. and Listeria monocytogenes isolated from fish, shrimp, and cooked ready-to-eat (RTE) aquatic products in Iran

“…They also reported one (2.5%) positive shrimp sample for Listeria contamination. Based on results of Modaresi, Mardani, Tukmechi, and Ownagh (2011) in the city of Urmia (northwestern Iran) the incidence of all species of Listeria spp. and L. monocytogenes were 12.37% and 2.57% in raw fish, including farmed samples of Oncorhynchus mykiss, farmed Cyprinus carpio, farmed Hypophthalmichthys molitrix, Sander lucioperca, Abramis brama, Astacus leptodactylus, and Silurus glanis, respectively.…”

Prevalence, antimicrobial susceptibility and virulotyping of Listeria species and Listeria monocytogenes isolated from open-air fish markets

“…Previous reports by H Momtaz and S Yadollahi [ 43 ] and VS Parihar, S Barbuddhe, M-L Danielsson-Tham and W Tham [ 42 ], indicated contamination of 7.7 and 9 % of fish samples with the pathogen . L. monocytogenes has been isolated from fish and fish products in earlier studies in different provinces of Iran, including Khuzestan (4 %) [ 41 ], West Azarbaijan (2.6 %) [ 45 ], and Gilan (2.2 %) [ 46 ]. Furthermore, other reports has shown contamination of fish market environment samples with the pathogen in central part of Iran (16.5 %) and Northern Greece (5 %) [ 40 , 47 ].…”

Molecular characterization of Listeria monocytogenes isolated from fresh seafood samples in Iran

“…Also, Akhondzadeh Basti et al [ 37 ] reported that 2.6% of smoked fish samples were positive for L. monocytogenes . Study in Urumia, Iran showed that 12.37% of collected fish samples were positive for Listeria [ 39 ]. They showed that 21% and 29% of isolates were L. monocytogenes and L. ivonoi [ 39 ].…”

“…Study in Urumia, Iran showed that 12.37% of collected fish samples were positive for Listeria [ 39 ]. They showed that 21% and 29% of isolates were L. monocytogenes and L. ivonoi [ 39 ]. The results of our study and several Iranian reports showed that the Listeria spp.…”