Molecular characterization of Listeria monocytogenes isolated from fresh seafood samples in Iran

Momtaz, Hassan; Yadollahi, Shole

doi:10.1186/1746-1596-8-149

Cited by 43 publications

(31 citation statements)

References 38 publications

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“…1). The findings in the present study are in agreement with the work carried out by Momtaz and Yadollahi (2013) who reported the presence of these genes in 18 isolates recovered from marine food samples.…”

Section: Resultssupporting

confidence: 83%

“…The production of haemolysin is also an important characteristic which is directly related to the pathogenicity of Listeria, since non haemolytic Listeria species can be considered as non-pathogenic (Courtieu, 1991). Momtaz and Yadollahi (2013) reported characteristic enhanced haemolytic zone with S. aureus on SBA in L. monocytogenes isolates obtained from seafood. The potentials of CAMP test as in-vitro pathogenicity assay has been exploited by many workers including Jallewar et al (2007) and Yadav et al (2010).…”

Section: Resultsmentioning

confidence: 99%

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Molecular Characterization of Listeria monocytogenes in white meat samples from Punjab, India

Kaur¹,

Singh²,

Sran³

et al. 2017

IJAR

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A study was undertaken to assess the prevalence of Listeria monocytogenes in white meats in the Punjab, India. A total of 335 samples including 115 samples of chicken, 75 samples of pork and 145 samples of fish were collected from retail outlets. Isolation of the pathogen was done by selective enrichment in University of Vermont Medium I and II and plating onto PALCAM agar. The recovered Listeria isolates were subjected to in-vitro pathogenicity assays and multiplex PCR targeting virulence-associated genes (prfA, plcA, actA, hlyA and iap) of L. monocytogenes. A total of thirty one (31) L. monocytogenes strains were isolated from meat samples with the prevalence of L. monocytogenes as 9.2%. Maximum prevalence was seen in fish (19.3%) followed by chicken (1.7%) and pork (1.3%). All isolates recovered from the study exhibited pathogenicity in in-vitro pathogenicity assays as well as possessed all the virulence related genes. Thus, the presence of pathogenic strains of L. monocytogenes in white meats with a prevalence of 9.2% appeared to be a cause for concern with profound public health implications.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Listeria monocytogenes in white meat samples from Punjab, India

Kaur¹,

Singh²,

Sran³

et al. 2017

IJAR

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“…Camp, Piplc Assay with Protein Profiling and Antibiotic Resistance Recovered from Nagpur Region since non-haemolytic Listeria species can be considered as non-pathogenic (FAO/WHO/OIE, 2008) and the pathogenicity of the pathogen is highly correlated to the haemolytic factor (Yadav et al, 2010;Momtaz and Yadollahi, 2013 In CAMP test, haemolysin acts synergistically with the β-haemolysis of S. aureus on sheep erythrocytes and gives synergistic zone of hemolysis towards S. aureus which is due to either phosphatidylinositol specific or phosphatidylcholine specific C from L. monocytogenes and sphingomyelinase from Staphylococcus aureus (Farber and Peterkin, 1991;McKellar, 1994). Evaluation of the pathogenicity of Listeria species by phosphatidylinositol specific phospholipase C (PI-PLC) based assay has been reported to be reliable indicator to discriminate pathogenic and non-pathogenic Listeria species (Notermans et al, 1991).…”

Section: Invitro Characterization Of Listeria Monocytogenes Isolates mentioning

confidence: 99%

In-Vitro Characterization Of Listeria Monocytogenes Isolates By Haemolysis, Camp, Piplc Assay With Protein Profiling And Antibiotic Resistance Recovered From Nagpur Region

Dudhe¹

2014

Adv. Anim. Vet. Sci.

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Listeria monocytogenes is one of the most virulent foodborne zoonotic pathogen with 20 to 30% of clinical infections resulting in death. The L. monocytogenes is ubiquitous in the nature and has been isolated from a variety of foods and environmental sources infecting human and animals revealing the pathogenicity of organism. Reports are very few regarding the pathogenesis of organism in Nagpur region, Maharashtra, India. So the present study was proposed to evaluate the pathogenicity of 12 L.monocytogenes isolates recovered from foods of animal origin (Chevon, pork, beef and carabeef) collected in and around Nagpur city. In vitro expression of pathogenisity was carried out by using viz., Haemolysis on Sheep blood agar (SBA), Christie Atkins Munch Petersen (CAMP) Test, Phosphatidylinositol-specific phospholipase-C (PI-PLC) assay along with protein profiling and subsequently studied for their pattern of antibiotic resistance. All 12 isolates of L. monocytogenes were found haemolytic in nature and showed PIPLC activity with variation in degree of production which designated them as pathogenic one. The protein profiles of all isolates exhibited minimum 25 to maximum 29 bands where protein bands of molecular weight from 56.237 kDa to 60.33 kDa and 29.226 kDa to 33.48 kDa shared by all isolates can be correlated with the haemolytic LLO (Listerolysin-O) and PI-PLC activity respectively. In antibiotic assay, most isolates showed multidrug resistance, besides no resistance was recorded towards Gentamycin, Norfloxacin, Ampicilin and Cefotaxime. The study revealed a high virulence nature of isolates of L. monocytogenes among food animals in and around Nagpur region. Haemolysin on SBA, PI-PLC activity along with exhibition of polypeptide in the range for these activities can be suggestive of protein profiling as technique for determination of pathogenicity.All copyrights reserved to Nexus® academic publishers

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“…are Gram positive, and facultative anaerobic organisms. They are also non-spore forming, and rod-shaped bacteria (Momtaz and Yadollahi, 2013;Odetokun and Adetunji, 2017). The genus Listeria has been divided so far into 17 species and 4 subtypes on the basis of 16S rRNA sequences (Anon., 2017).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Characterization of Listeria Species from Raw Milk and Dairy Products from Çanakkale Province

Şanlibaba

Tezel

2018

Turkish JAF Sci.Tech.

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The objective of this study was to determine the prevalence of Listeria species, specifically Listeria monocytogenes, in raw milk, pasteurized milk, white cheese, and homemade cheese. A total of 200 food samples were collected and analyzed to examine the presence of Listeria spp. The EN ISO 11290-1 method was used for isolation of Listeria. API Listeria test kit was used for biochemically characterization. Listeria spp. were isolated in 25 of the 200 samples (12.5%). The largest number of Listeria spp. was detected in homemade cheese (24%), followed by raw milk (18%), and white cheese (8%). Listeria spp. were not isolated from the pasteurized milk. The most common species isolated were Listeria innocua (5.5%); the remaining Listeria isolates were Listeria ivanovi (3.5%), Listeria welshimeri (3%), and Listeria monocytogenes (0.5%). Listeria monocytogenes was detected in only raw milk.

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Molecular characterization of Listeria monocytogenes isolated from fresh seafood samples in Iran

Cited by 43 publications

References 38 publications

Molecular Characterization of Listeria monocytogenes in white meat samples from Punjab, India

Molecular Characterization of Listeria monocytogenes in white meat samples from Punjab, India

In-Vitro Characterization Of Listeria Monocytogenes Isolates By Haemolysis, Camp, Piplc Assay With Protein Profiling And Antibiotic Resistance Recovered From Nagpur Region

Prevalence and Characterization of Listeria Species from Raw Milk and Dairy Products from Çanakkale Province

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