Prevalence ofStreptococcus dysgalactiaesubsp.equisimilisandS. equisubsp.zooepidemicusin a sample of healthy dogs, cats and horses

Acke, Els; Midwinter, Anne C.; Lawrence, K.E.; Gordon, Stuart; Moore, Sarah A.; Rasiah, I; Steward, Karen F.; French, Nigel P.; Waller, Andrew S.

doi:10.1080/00480169.2015.1016133

Cited by 29 publications

(19 citation statements)

References 41 publications

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“…The risk of zoonotic infection is the most significant in people having regular contact with animals, such as veterinarians, cattlemen, and farmers. SDSE is also prevalent on a skin of healthy dogs, cats, and horses [1], which poses a risk also to people having contact with domestic (companion) animals.…”

Section: Discussionmentioning

confidence: 99%

“…Until recently, it has been considered as an exclusive animal pathogen. Nowadays, it is responsible for both animal infections in wild animals, pets, and livestock [1–4] and human infections often clinically similar to the ones caused by group A streptococcus ( Streptococcus pyogenes ) [5–7]. According to the outline of evolutionary changes involved in crossing an animal-to-human interspecies barrier, presented by Wolfe et al in Nature [8], SDSE is probably located on the second stage of this process—it is being isolated from humans, what has already been reported [9, 10]; however, there is no evidence for human-to-human transfer.…”

Section: Introductionmentioning

confidence: 99%

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Potential Factors Enabling Human Body Colonization by Animal Streptococcus dysgalactiae subsp. equisimilis Strains

Ciszewski

Szewczyk

2017

Curr Microbiol

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Streptococcus dysgalactiae subsp. equisimilis (SDSE) is a pyogenic, Lancefield C or G streptococcal pathogen. Until recently, it has been considered as an exclusive animal pathogen. Nowadays, it is responsible for both animal infections in wild animals, pets, and livestock and human infections often clinically similar to the ones caused by group A streptococcus (Streptococcus pyogenes). The risk of zoonotic infection is the most significant in people having regular contact with animals, such as veterinarians, cattlemen, and farmers. SDSE is also prevalent on skin of healthy dogs, cats, and horses, which pose a risk also to people having contact with companion animals. The main aim of this study was to evaluate if there are features differentiating animal and human SDSE isolates, especially in virulence factors involved in the first stages of pathogenesis (adhesion and colonization). Equal groups of human and animal SDSE clinical strains were obtained from superficial infections (skin, wounds, abscesses). The presence of five virulence genes (prtF1, prtF2, lmb, cbp, emm type) was evaluated, as well as ability to form bacterial biofilm and produce BLIS (bacteriocin-like inhibitory substances) which are active against human skin microbiota. The study showed that the presence of genes coding for fibronectin-binding protein and M protein, as well as BLIS activity inhibiting the growth of Corynebacterium spp. strains might constitute the virulence factors which are necessary to colonize human organism, whereas they are not crucial in animal infections. Those virulence factors might be horizontally transferred from human streptococci to animal SDSE strains, enabling their ability to colonize human organism.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potential Factors Enabling Human Body Colonization by Animal Streptococcus dysgalactiae subsp. equisimilis Strains

Ciszewski

Szewczyk

2017

Curr Microbiol

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“…These S. dysgalactiae subsp. equisimilis strains are zoonotic and are carried in both humans and animals, including house pets and horses (10,11).…”

Section: Introductionmentioning

confidence: 99%

Species Identification of β-Hemolytic Streptococci from Diseased Companion Animals and Their Antimicrobial Resistance Data in Japan (2017)

et al. 2019

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This study aimed to identify the species and assess the antimicrobial resistance (AMR) of β-hemolytic streptococci isolated from companion animals in Japan. Strains were isolated from clinical specimens of 131 companion animals that exhibited symptoms in April-May 2017. We identified strains by 16S rRNA sequencing and assessed their antimicrobial susceptibility using the broth microdilution method. AMR genes erm(A)-erm(B)-mef(A) and tet(M)-tet(O)-tet(K)-tet(L)-tet(S) in all isolates were amplified by PCR. 16S rRNA sequencing identified β-hemolytic streptococcal species as Streptococcus canis (n = 117, 89.3%), S. agalactiae (n = 7), S. dysgalactiae subsp. equisimilis (n = 5), S. dysgalactiae subsp. dysgalactiae (n = 1), and S. equi subsp. zooepidemicus (n = 1). Overall AMR rates were 39.7% for minocycline, 19.8% for erythromycin, and 17.6% for clindamycin, with a minimum inhibitory concentration (MIC 90) of > 4, > 2, and > 1 μg/mL, respectively. AMR genotyping showed the presence of single or mixed types: erm(B)-mef(A) and tet(M)-tet(O)-tet(L)-tet(S). There was a significant relationship between tetracycline-resistance genotypes and open pus/skin-derived specimens. These observations identify some unique features of β-hemolytic streptococcal isolates from companion animals in Japan, such as the dominant isolation of S. canis and resistance to tetracycline, macrolide, and lincosamide antibiotics, in terms of species identification and AMR properties.

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“…Streptococcus canis was the most frequently beta‐haemolytic Streptococcus isolated in our study. These bacteria were previously isolated by other researches from the vagina of bitches in heat (Allen & Dagnall, ; Watts et al., ); and Streptococcus dysgalactiae was previously isolated from the canine pharynx (Acke et al., ; Schrieber, Towers, Muscatello, & Speare, ). However, according to our knowledge, it has not been isolated from the canine reproductive tract.…”

Section: Discussionmentioning

confidence: 93%

Vaginal isolation of beta‐haemolytic Streptococcus from bitches with and without neonatal deaths in the litters

Guerrero¹,

Stornelli²,

Jurado³

et al. 2018

Reprod Domestic Animals

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The aim of the study was to identify beta-haemolytic streptococci in the vagina of bitches who had delivered healthy litters and bitches who had delivered litters in which neonatal deaths occurred. Fifty-one bitches divided into two groups were used. Group 1 (G1) included 28 bitches that had delivered healthy litters and group 2 (G2) included 23 bitches that had delivered puppies who died in the neonatal period. Two vaginal samples were taken, one in proestrus and the other at the end of gestation (EG). Beta-haemolytic Streptococcus (BS) was isolated from 16 bitches (57%) in G1 and from 21 bitches (91%) in G2. The bacteriological cultures, serological tests (Streptex ) and PCR assay allowed identification of Streptococcus canis and Streptococcus dysgalactiae in G1 and G2. Ultramicroscopic studies allowed the observation of M Protein and capsules in strains of S. dysgalactiae and S. canis in G1 and G2. The S. canis strains isolated from G2 showed thicker capsules than S. canis strains isolated from G1 (234 ± 24.2 vs 151.23 ± 28.93 nm; p < .001.). No differences were observed in capsule thickness between strains of S. dysgalactiae isolated from G1 and G2 (210 ± 13.54 vs 211.66 ± 19.67 nm; p > .70). All strains of beta-haemolytic Streptococcus isolated were penicillin sensitive. Penicillin was administered from EG to 5 days post-partum in 10 G2 females with isolation of BS (G2A). Saline solution was administered in eleven G2 females with isolation of BS (G2B). Ninety per cent of the puppies survived in G2A and 25% survived in G2B. Our results suggest BS is involved in canine neonatal deaths.

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Prevalence ofStreptococcus dysgalactiaesubsp.equisimilisandS. equisubsp.zooepidemicusin a sample of healthy dogs, cats and horses

Cited by 29 publications

References 41 publications

Potential Factors Enabling Human Body Colonization by Animal Streptococcus dysgalactiae subsp. equisimilis Strains

Potential Factors Enabling Human Body Colonization by Animal Streptococcus dysgalactiae subsp. equisimilis Strains

Species Identification of β-Hemolytic Streptococci from Diseased Companion Animals and Their Antimicrobial Resistance Data in Japan (2017)

Vaginal isolation of beta‐haemolytic Streptococcus from bitches with and without neonatal deaths in the litters

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