Species Identification of β-Hemolytic Streptococci from Diseased Companion Animals and Their Antimicrobial Resistance Data in Japan (2017)

Abstract

This study aimed to identify the species and assess the antimicrobial resistance (AMR) of β-hemolytic streptococci isolated from companion animals in Japan. Strains were isolated from clinical specimens of 131 companion animals that exhibited symptoms in April-May 2017. We identified strains by 16S rRNA sequencing and assessed their antimicrobial susceptibility using the broth microdilution method. AMR genes erm(A)-erm(B)-mef(A) and tet(M)-tet(O)-tet(K)-tet(L)-tet(S) in all isolates were amplified by PCR. 16S rRNA sequencing identified β-hemolytic streptococcal species as Streptococcus canis (n = 117, 89.3%), S. agalactiae (n = 7), S. dysgalactiae subsp. equisimilis (n = 5), S. dysgalactiae subsp. dysgalactiae (n = 1), and S. equi subsp. zooepidemicus (n = 1). Overall AMR rates were 39.7% for minocycline, 19.8% for erythromycin, and 17.6% for clindamycin, with a minimum inhibitory concentration (MIC 90) of > 4, > 2, and > 1 μg/mL, respectively. AMR genotyping showed the presence of single or mixed types: erm(B)-mef(A) and tet(M)-tet(O)-tet(L)-tet(S). There was a significant relationship between tetracycline-resistance genotypes and open pus/skin-derived specimens. These observations identify some unique features of β-hemolytic streptococcal isolates from companion animals in Japan, such as the dominant isolation of S. canis and resistance to tetracycline, macrolide, and lincosamide antibiotics, in terms of species identification and AMR properties.

Analysis of the Type II-A CRISPR-Cas System in <i>Streptococcus canis</i> Isolated from Diseased Companion Animals and One Human Patient in Japan

“…Nineteen pet origin S. canis isolates, which were identified by means of 16S rRNA sequencing data and amplification of a S. canis-specific gene (cfg, encoding cohemolysin: CAMP-factor) were selected from our stored strains that were provided by the Sanritsu Zelkova Veterinary Laboratory (strain collection period: April 2017) (11). The current study was approved by the Ethics Committee of the Veterinary Laboratory (approval number SZ20170316) to ensure privacy of the affected pets.…”

Novel Quinolone Nonsusceptible <i>Streptococcus canis</i> Strains with Point Mutations in Quinolone Resistance-Determining Regions and Their Related Factors

“…During previous studies (18,19), we collected 68 and 117 S. canis isolates in 2015 and 2017, respectively, from diseased companion animals along, with the animal information. We identified the S. canis strains at the species level based on 16S rRNA sequencing data (18,19). We also validated the accuracy of the identification through…”

Draft Genome Sequence of Blood-Origin Streptococcus canis Strain FU149, Isolated from a Dog with Necrotizing Soft Tissue Infection

“… Streptococcus canis , which was first proposed in 1986 ( 1 ), is characterized as having beta-hemolysis activity and as Lancefield carbohydrate antigen group G. This bacterium can cause mild to severe infections in humans and animals ( 2 – 5 ). Here, we report the draft genome sequence of an S. canis strain isolated from the blood of a miniature sausage dog (male, 13 years old, born in Hiroshima City, Japan) with a necrotizing soft tissue infection.…”

“…The blood-origin strain FU149 was isolated using the blood culture system VersaTrek (Kohjin-Bio, Japan) ( 6 ). Isolates were inoculated onto sheep blood agar plates and incubated in 5% CO 2 at 35°C for 24 h ( 3 ). Colonies with a gray-white smooth appearance (indicating beta-hemolysis) were further grown overnight in Todd-Hewitt broth supplemented with yeast extract.…”