Prevalence of <i>Escherichia Coli</i> O157 and Other Shiga-Toxin-Producing <i>E. Coli</i> in Lambs at Slaughter

McCluskey, Brian J.; Rice, Daniel H.; Hancock, Dale D.; Hovde, Carolyn J.; Besser, Thomas E.; Gray, Steacy; Johnson, Roger P.

doi:10.1177/104063879901100619

Cited by 22 publications

(25 citation statements)

References 21 publications

(34 reference statements)

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“…Observed prevalence of E. coli O157:H7 at abattoir level (2.8%) is in line with the findings from Ethiopia [7], South Africa [10], United Kingdom [11, 12] and Ireland [13] which reported 2.7, 2.8, 2.9, 3.2 and 3.0%, respectively. Compared with the present study, higher prevalence of E. coli O157:H7 have been also reported (8% from Ethiopia [5] and 6.4–9.6% from Iran [14–16].…”

Section: Discussionsupporting

confidence: 88%

Occurrence of Escherichia coli O157:H7 in cattle feces and contamination of carcass and various contact surfaces in abattoir and butcher shops of Hawassa, Ethiopia

et al. 2017

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BackgroundDespite of the sanitation measures in municipal abattoirs to reduce contamination, Escherichia coli continues to be a health hazard. The present study was conducted on 150 apparently healthy slaughtered cattle at municipal abattoir and in 50 different butcher shops in Hawassa town, Ethiopia. The objectives of the study were investigating the occurrence and antimicrobial resistance of E. coli O157:H7 isolated from fecal samples, carcasses swab, contacts surfaces (swabs of meat handlers hands, knife and clothes of meat transporters) as well as from butcher shops (meat samples, swabs from cutting board swab, butcher men hand and knife surface). E. coli O157:H7 was isolated and identified using bacteriological culture, biochemical tests and Biolog identification system. All E. coli O157:H7 isolates were then checked for their antimicrobial susceptibility pattern using eleven selected antimicrobial discs.ResultsOf the entire set of 630 samples, 2.4% (15/630) (95% CI = 1.3–3.9%) were positive for E. coli O157:H7. When disaggregated by the sources of the samples, E. coli O157:H7 were prevalent in 2.8% (11 of 390) of the abattoir samples, of which 4.7% of the fecal sample and 2.7% of the carcass swabs. And E. coli O157:H7 were positive in 1.7% (4 of 240) of butcher shop specimens of which 2% of meat sample and 3.3% of Cutting board swabs. No statistically significant difference in the prevalence of E. coli 0157: H7 between sex, origin, and breed of cattle. The isolated E. coli O157:H7 were found to be100% susceptible to cefotaxime, ceftriaxone, gentamycin, kanamycin and nalidixic acid.ConclusionThis study concludes the occurrence of E. coli O157:H7 and the presence of multiple antibiotic resistance profiles in cattle slaughtered at Hawassa municipal abattoir and retail meat sold at butcher shops. This indicates high risk to public health especially in Ethiopia where many people consume raw or under cooked meat. Regulatory control of antibiotics usage in livestock production and pharmaco-epidemiological surveillance in food animals and animal products is hereby recommended to ensure consumer safety.

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Section: Discussionsupporting

confidence: 88%

Occurrence of Escherichia coli O157:H7 in cattle feces and contamination of carcass and various contact surfaces in abattoir and butcher shops of Hawassa, Ethiopia

et al. 2017

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“…However, the Scottish farm-based study (25) covered a period of 2 years and showed a group-level prevalence of 23.7% (95% CI, 21.0 to 26.5%), which is significantly lower than that reported here. The reasons for this difference may be because the present study was performed in the summer months, which is defined as the period of high prevalence by Hancock et al (9), because the animals may have shed increasing loads of E. coli O157 from stress due to transport prior to slaughter (14), or because larger sample volumes were assayed in the present project (25 g compared to 1 g).…”

Section: Discussionmentioning

confidence: 80%

Concentration and Prevalence of Escherichia coli O157 in Cattle Feces at Slaughter

Omisakin¹,

MacRae

Ogden

et al. 2003

Appl Environ Microbiol

269

202

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The concentration and prevalence of Escherichia coli O157 in cattle feces at the time of slaughter was studied over a 9-week period from May to July 2002. Fecal samples (n ‫؍‬ 589) were collected from the rectums of slaughtered cattle, and the animal-level prevalence rate was estimated to be 7.5% (95% confidence interval [CI], 5.4 to 9.6%) while the group prevalence was 40.4% (95% CI, 27.7 to 53.2%). Of the 44 infected animals detected, 9% were high shedders that contained E. coli O157 at concentrations of >10 4 CFU g ؊1 . These 9% represented >96% of the total E. coli O157 produced by all animals tested. All isolates possessed the vt 2 gene, 39 had the eaeA gene, and a further five had the vt 1 gene also. The presence of high-shedding animals at the abattoir increases the potential risk of meat contamination during the slaughtering process and stresses the need for correctly implemented hazard analysis and critical control point procedures.Escherichia coli O157 was first identified as a food-borne pathogen in 1982 during an outbreak that was traced to contaminated hamburgers (20). The pathogen is associated with a range of symptoms, including watery or bloody diarrhea, vomiting, hemorrhagic colitis, and hemolytic uremic syndrome, which is characterized by acute renal failure affecting mainly children and the immunocompromised (7). While the majority of foods linked to human outbreaks of E. coli O157 are not assessed quantitatively, some studies have indicated a low infective dose (1, 26), highlighting the need for stringent control of contamination during food production.Cattle and other ruminants have been established as major natural reservoirs for E. coli O157 (18) and play a significant role in the epidemiology of human infections (7). It has been estimated that 1 to 4% of United Kingdom cattle are infected at slaughter (3,19), although more recently a prevalence rate of 8.6% has been reported from a farm study in Scotland (25). In the United States, breeding herd prevalences of 1% (21) and 9.3% (6) have been recorded, whereas in feedlot animals, rates have varied between 2.8% (4) and 35.8% (5). Prevalences in the summer months were usually greater than in the winter months. A number of environmental and food-borne sources have caused E. coli O157 incidents, with many attributed to the consumption of food of bovine origin (22) or with either direct or indirect contact with cattle and other farm animals (13).The concentration at which E. coli O157 is shed in feces varies from animal to animal as demonstrated in a North American study with calves (29), where a range from 10 2 to 10 5 CFU g Ϫ1 was observed. High-shedding sheep (excreting Ͼ10 4 CFU g Ϫ1 ) were responsible for the New Deer E. coli O157 outbreak in Scotland (16,23). High-shedding animals pose an elevated risk of contaminating the food chain if presented to slaughter. However, little published data are available on the concentration of E. coli O157 in cattle feces at the time of slaughter.The health risk from E. coli O157 and other pathogens is mi...

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“…The prevalence of E. coli O157:H7 (2.65%) isolated from carcass in this study was in close agreement with the reported prevalence of 2.9% and 3.2% in the United Kingdom [16,17], respectively. 2% in Canada [18] and five isolates (2.8%) out of 180 meat and meat products examined in South Africa [19]. The lower carcass contamination might be due to low fecal prevalence of E. coli O157:H7 in these animals and operational activities in the slaughter house, which results in relatively low risk of contamination and cross contamination [5].…”

Section: Discussionmentioning

confidence: 99%

Study on Carcass Contaminating Escherichia coli in Apparently Healthy Slaughtered Cattle in Haramaya University Slaughter House with Special Emphasis on Escherichia coli O157:H7, Ethiopia

Terefe¹

2013

J Veterinar Sci Technol 2013

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Prevalence of Escherichia Coli O157 and Other Shiga-Toxin-Producing E. Coli in Lambs at Slaughter

Cited by 22 publications

References 21 publications

Occurrence of Escherichia coli O157:H7 in cattle feces and contamination of carcass and various contact surfaces in abattoir and butcher shops of Hawassa, Ethiopia

Occurrence of Escherichia coli O157:H7 in cattle feces and contamination of carcass and various contact surfaces in abattoir and butcher shops of Hawassa, Ethiopia

Concentration and Prevalence of Escherichia coli O157 in Cattle Feces at Slaughter

Study on Carcass Contaminating Escherichia coli in Apparently Healthy Slaughtered Cattle in Haramaya University Slaughter House with Special Emphasis on Escherichia coli O157:H7, Ethiopia

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